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Lambda Red recombinase-mediated integration of the high molecular weight DNA into the Escherichia coli chromosome

BACKGROUND: Escherichia coli K-12 is a frequently used host for a number of synthetic biology and biotechnology applications and chassis for the development of the minimal cell factories. Novel approaches for integrating high molecular weight DNA into the E. coli chromosome would therefore greatly f...

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Detalles Bibliográficos
Autores principales: Juhas, Mario, Ajioka, James W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5050610/
https://www.ncbi.nlm.nih.gov/pubmed/27716307
http://dx.doi.org/10.1186/s12934-016-0571-y