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Cirrhosis related functionality characteristic of the fecal microbiota as revealed by a metaproteomic approach

BACKGROUND: Intestinal microbiota operated as a whole and was closely related with human health. Previous studies had suggested close relationship between liver cirrhosis (LC) and gut microbiota. METHODS: To determine the functional characteristic of the intestinal microbiota specific for liver cirr...

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Autores principales: Wei, Xiao, Jiang, Shan, Chen, Yuye, Zhao, Xiangna, Li, Huan, Lin, Weishi, Li, Boxing, Wang, Xuesong, Yuan, Jing, Sun, Yansong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5051048/
https://www.ncbi.nlm.nih.gov/pubmed/27716148
http://dx.doi.org/10.1186/s12876-016-0534-0
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author Wei, Xiao
Jiang, Shan
Chen, Yuye
Zhao, Xiangna
Li, Huan
Lin, Weishi
Li, Boxing
Wang, Xuesong
Yuan, Jing
Sun, Yansong
author_facet Wei, Xiao
Jiang, Shan
Chen, Yuye
Zhao, Xiangna
Li, Huan
Lin, Weishi
Li, Boxing
Wang, Xuesong
Yuan, Jing
Sun, Yansong
author_sort Wei, Xiao
collection PubMed
description BACKGROUND: Intestinal microbiota operated as a whole and was closely related with human health. Previous studies had suggested close relationship between liver cirrhosis (LC) and gut microbiota. METHODS: To determine the functional characteristic of the intestinal microbiota specific for liver cirrhosis, the fecal metaproteome of three LC patients with Child-Turcotte-Pugh (CTP) score of A, B, and C, and their spouse were first compared using high-throughput approach based on denaturing polyacrylamide gel electrophoresis and liquid chromatography–tandem mass spectrometry in our study. RESULTS: A total of 5,020 proteins (88 % from bacteria, 12 % form human) were identified and annotated based on the GO and KEGG classification. Our results indicated that the LC patients possessed a core metaproteome including 119 proteins, among which 14 proteins were enhanced expressed and 7 proteins were unique for LC patients compared with the normal, which were dominant at the function of carbohydrate metabolism. In addition, LC patients have unique biosynthesis of branched chain amino acid (BCAA), pantothenate, and CoA, enhanced as CTP scores increased. Those three substances were all important in a wide array of key and essential biological roles of life. CONCLUSIONS: We observed a highly comparable cirrhosis-specific metaproteome clustering of fecal microbiota and provided the first supportive evidence for the presence of a LC-related substantial functional core mainly involved in carbohydrate, BCAA, pantothenate, and CoA metabolism, suggesting the compensation of intestinal microbiota for the fragile and innutritious body of cirrhotic patients. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12876-016-0534-0) contains supplementary material, which is available to authorized users.
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spelling pubmed-50510482016-10-06 Cirrhosis related functionality characteristic of the fecal microbiota as revealed by a metaproteomic approach Wei, Xiao Jiang, Shan Chen, Yuye Zhao, Xiangna Li, Huan Lin, Weishi Li, Boxing Wang, Xuesong Yuan, Jing Sun, Yansong BMC Gastroenterol Research Article BACKGROUND: Intestinal microbiota operated as a whole and was closely related with human health. Previous studies had suggested close relationship between liver cirrhosis (LC) and gut microbiota. METHODS: To determine the functional characteristic of the intestinal microbiota specific for liver cirrhosis, the fecal metaproteome of three LC patients with Child-Turcotte-Pugh (CTP) score of A, B, and C, and their spouse were first compared using high-throughput approach based on denaturing polyacrylamide gel electrophoresis and liquid chromatography–tandem mass spectrometry in our study. RESULTS: A total of 5,020 proteins (88 % from bacteria, 12 % form human) were identified and annotated based on the GO and KEGG classification. Our results indicated that the LC patients possessed a core metaproteome including 119 proteins, among which 14 proteins were enhanced expressed and 7 proteins were unique for LC patients compared with the normal, which were dominant at the function of carbohydrate metabolism. In addition, LC patients have unique biosynthesis of branched chain amino acid (BCAA), pantothenate, and CoA, enhanced as CTP scores increased. Those three substances were all important in a wide array of key and essential biological roles of life. CONCLUSIONS: We observed a highly comparable cirrhosis-specific metaproteome clustering of fecal microbiota and provided the first supportive evidence for the presence of a LC-related substantial functional core mainly involved in carbohydrate, BCAA, pantothenate, and CoA metabolism, suggesting the compensation of intestinal microbiota for the fragile and innutritious body of cirrhotic patients. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12876-016-0534-0) contains supplementary material, which is available to authorized users. BioMed Central 2016-10-04 /pmc/articles/PMC5051048/ /pubmed/27716148 http://dx.doi.org/10.1186/s12876-016-0534-0 Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Wei, Xiao
Jiang, Shan
Chen, Yuye
Zhao, Xiangna
Li, Huan
Lin, Weishi
Li, Boxing
Wang, Xuesong
Yuan, Jing
Sun, Yansong
Cirrhosis related functionality characteristic of the fecal microbiota as revealed by a metaproteomic approach
title Cirrhosis related functionality characteristic of the fecal microbiota as revealed by a metaproteomic approach
title_full Cirrhosis related functionality characteristic of the fecal microbiota as revealed by a metaproteomic approach
title_fullStr Cirrhosis related functionality characteristic of the fecal microbiota as revealed by a metaproteomic approach
title_full_unstemmed Cirrhosis related functionality characteristic of the fecal microbiota as revealed by a metaproteomic approach
title_short Cirrhosis related functionality characteristic of the fecal microbiota as revealed by a metaproteomic approach
title_sort cirrhosis related functionality characteristic of the fecal microbiota as revealed by a metaproteomic approach
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5051048/
https://www.ncbi.nlm.nih.gov/pubmed/27716148
http://dx.doi.org/10.1186/s12876-016-0534-0
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