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Spatial and compositional variation in the fungal communities of organic and conventionally grown apple fruit at the consumer point-of-purchase
The fungal diversity in harvested apples from organic or conventional management practices was analyzed in different fruit locations (stem end, calyx end, peel, and wounded flesh) shortly after fruit purchase (T1) and after 2 weeks of storage (T5). A total of 5,760,162 high-quality fungal sequences...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5051542/ https://www.ncbi.nlm.nih.gov/pubmed/27766161 http://dx.doi.org/10.1038/hortres.2016.47 |
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author | Abdelfattah, Ahmed Wisniewski, Michael Droby, Samir Schena, Leonardo |
author_facet | Abdelfattah, Ahmed Wisniewski, Michael Droby, Samir Schena, Leonardo |
author_sort | Abdelfattah, Ahmed |
collection | PubMed |
description | The fungal diversity in harvested apples from organic or conventional management practices was analyzed in different fruit locations (stem end, calyx end, peel, and wounded flesh) shortly after fruit purchase (T1) and after 2 weeks of storage (T5). A total of 5,760,162 high-quality fungal sequences were recovered and assigned to 8,504 Operational Taxonomic Units. Members of the phylum Ascomycota were dominant in all samples and accounted for 91.6% of the total number of detected sequences. This was followed by Basidiomycota (8%), Chytridiomycota (0.1%), and unidentified fungi (0.3%). Alpha and beta diversity analyses revealed the presence of significantly different fungal populations in the investigated fruit parts. Among detected fungi, the genus Penicillium prevailed in the peel and in the wounded flesh while Alternaria spp. prevailed in the calyx and stem end samples that included apple core tissues. Several taxonomic units that appear to be closely related to pathogenic fungi associated with secondary human infections were present in peel and wounds. Moreover, significantly different populations were revealed in organic and conventional apples and this result was consistent in all investigated fruit parts (calyx end, peel, stem end, and wounded flesh). Several unique taxa were exclusively detected in organic apples suggesting that management practices may have been a contributing factor in determining the taxa present. In contrast, little differences were revealed in the two assessment times (T1 and T5). Results of the present study represent an advancement of the current knowledge on the fungal microbiota in collected fruit tissues of apple. |
format | Online Article Text |
id | pubmed-5051542 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-50515422016-10-20 Spatial and compositional variation in the fungal communities of organic and conventionally grown apple fruit at the consumer point-of-purchase Abdelfattah, Ahmed Wisniewski, Michael Droby, Samir Schena, Leonardo Hortic Res Article The fungal diversity in harvested apples from organic or conventional management practices was analyzed in different fruit locations (stem end, calyx end, peel, and wounded flesh) shortly after fruit purchase (T1) and after 2 weeks of storage (T5). A total of 5,760,162 high-quality fungal sequences were recovered and assigned to 8,504 Operational Taxonomic Units. Members of the phylum Ascomycota were dominant in all samples and accounted for 91.6% of the total number of detected sequences. This was followed by Basidiomycota (8%), Chytridiomycota (0.1%), and unidentified fungi (0.3%). Alpha and beta diversity analyses revealed the presence of significantly different fungal populations in the investigated fruit parts. Among detected fungi, the genus Penicillium prevailed in the peel and in the wounded flesh while Alternaria spp. prevailed in the calyx and stem end samples that included apple core tissues. Several taxonomic units that appear to be closely related to pathogenic fungi associated with secondary human infections were present in peel and wounds. Moreover, significantly different populations were revealed in organic and conventional apples and this result was consistent in all investigated fruit parts (calyx end, peel, stem end, and wounded flesh). Several unique taxa were exclusively detected in organic apples suggesting that management practices may have been a contributing factor in determining the taxa present. In contrast, little differences were revealed in the two assessment times (T1 and T5). Results of the present study represent an advancement of the current knowledge on the fungal microbiota in collected fruit tissues of apple. Nature Publishing Group 2016-10-05 /pmc/articles/PMC5051542/ /pubmed/27766161 http://dx.doi.org/10.1038/hortres.2016.47 Text en Copyright © 2016 The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Abdelfattah, Ahmed Wisniewski, Michael Droby, Samir Schena, Leonardo Spatial and compositional variation in the fungal communities of organic and conventionally grown apple fruit at the consumer point-of-purchase |
title | Spatial and compositional variation in the fungal communities of organic and conventionally grown apple fruit at the consumer point-of-purchase |
title_full | Spatial and compositional variation in the fungal communities of organic and conventionally grown apple fruit at the consumer point-of-purchase |
title_fullStr | Spatial and compositional variation in the fungal communities of organic and conventionally grown apple fruit at the consumer point-of-purchase |
title_full_unstemmed | Spatial and compositional variation in the fungal communities of organic and conventionally grown apple fruit at the consumer point-of-purchase |
title_short | Spatial and compositional variation in the fungal communities of organic and conventionally grown apple fruit at the consumer point-of-purchase |
title_sort | spatial and compositional variation in the fungal communities of organic and conventionally grown apple fruit at the consumer point-of-purchase |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5051542/ https://www.ncbi.nlm.nih.gov/pubmed/27766161 http://dx.doi.org/10.1038/hortres.2016.47 |
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