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The Relationship between Mating System and Genetic Diversity in Diploid Sexual Populations of Cyrtomium falcatum in Japan

The impact of variation in mating system on genetic diversity is a well-debated topic in evolutionary biology. The diploid sexual race of Cyrtomium falcatum (Japanese holly fern) shows mating system variation, i.e., it displays two different types of sexual expression (gametangia formation) in gamet...

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Autores principales: Imai, Ryosuke, Tsuda, Yoshiaki, Matsumoto, Sadamu, Ebihara, Atsushi, Watano, Yasuyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5051678/
https://www.ncbi.nlm.nih.gov/pubmed/27706257
http://dx.doi.org/10.1371/journal.pone.0163683
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author Imai, Ryosuke
Tsuda, Yoshiaki
Matsumoto, Sadamu
Ebihara, Atsushi
Watano, Yasuyuki
author_facet Imai, Ryosuke
Tsuda, Yoshiaki
Matsumoto, Sadamu
Ebihara, Atsushi
Watano, Yasuyuki
author_sort Imai, Ryosuke
collection PubMed
description The impact of variation in mating system on genetic diversity is a well-debated topic in evolutionary biology. The diploid sexual race of Cyrtomium falcatum (Japanese holly fern) shows mating system variation, i.e., it displays two different types of sexual expression (gametangia formation) in gametophytes: mixed (M) type and separate (S) type. We examined whether there is variation in the selfing rate among populations of this species, and evaluated the relationship between mating system, genetic diversity and effective population size using microsatellites. In this study, we developed eight new microsatellite markers and evaluated genetic diversity and structure of seven populations (four M-type and three S-type). Past effective population sizes (Ne) were inferred using Approximate Bayesian computation (ABC). The values of fixation index (F(IS)), allelic richness (A(R)) and gene diversity (h) differed significantly between the M-type (F(IS): 0.626, A(R): 1.999, h: 0.152) and the S-type (F(IS): 0.208, A(R): 2.718, h: 0.367) populations (when admixed individuals were removed from two populations). Although evidence of past bottleneck events was detected in all populations by ABC, the current N(e) of the M-type populations was about a third of that of the S-type populations. These results suggest that the M-type populations have experienced more frequent bottlenecks, which could be related to their higher colonization ability via gametophytic selfing. Although high population differentiation among populations was detected (F(ST) = 0.581, F’(ST) = 0.739), there was no clear genetic differentiation between the M- and S-types. Instead, significant isolation by distance was detected among all populations. These results suggest that mating system variation in this species is generated by the selection for single spore colonization during local extinction and recolonization events and there is no genetic structure due to mating system.
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spelling pubmed-50516782016-10-27 The Relationship between Mating System and Genetic Diversity in Diploid Sexual Populations of Cyrtomium falcatum in Japan Imai, Ryosuke Tsuda, Yoshiaki Matsumoto, Sadamu Ebihara, Atsushi Watano, Yasuyuki PLoS One Research Article The impact of variation in mating system on genetic diversity is a well-debated topic in evolutionary biology. The diploid sexual race of Cyrtomium falcatum (Japanese holly fern) shows mating system variation, i.e., it displays two different types of sexual expression (gametangia formation) in gametophytes: mixed (M) type and separate (S) type. We examined whether there is variation in the selfing rate among populations of this species, and evaluated the relationship between mating system, genetic diversity and effective population size using microsatellites. In this study, we developed eight new microsatellite markers and evaluated genetic diversity and structure of seven populations (four M-type and three S-type). Past effective population sizes (Ne) were inferred using Approximate Bayesian computation (ABC). The values of fixation index (F(IS)), allelic richness (A(R)) and gene diversity (h) differed significantly between the M-type (F(IS): 0.626, A(R): 1.999, h: 0.152) and the S-type (F(IS): 0.208, A(R): 2.718, h: 0.367) populations (when admixed individuals were removed from two populations). Although evidence of past bottleneck events was detected in all populations by ABC, the current N(e) of the M-type populations was about a third of that of the S-type populations. These results suggest that the M-type populations have experienced more frequent bottlenecks, which could be related to their higher colonization ability via gametophytic selfing. Although high population differentiation among populations was detected (F(ST) = 0.581, F’(ST) = 0.739), there was no clear genetic differentiation between the M- and S-types. Instead, significant isolation by distance was detected among all populations. These results suggest that mating system variation in this species is generated by the selection for single spore colonization during local extinction and recolonization events and there is no genetic structure due to mating system. Public Library of Science 2016-10-05 /pmc/articles/PMC5051678/ /pubmed/27706257 http://dx.doi.org/10.1371/journal.pone.0163683 Text en © 2016 Imai et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Imai, Ryosuke
Tsuda, Yoshiaki
Matsumoto, Sadamu
Ebihara, Atsushi
Watano, Yasuyuki
The Relationship between Mating System and Genetic Diversity in Diploid Sexual Populations of Cyrtomium falcatum in Japan
title The Relationship between Mating System and Genetic Diversity in Diploid Sexual Populations of Cyrtomium falcatum in Japan
title_full The Relationship between Mating System and Genetic Diversity in Diploid Sexual Populations of Cyrtomium falcatum in Japan
title_fullStr The Relationship between Mating System and Genetic Diversity in Diploid Sexual Populations of Cyrtomium falcatum in Japan
title_full_unstemmed The Relationship between Mating System and Genetic Diversity in Diploid Sexual Populations of Cyrtomium falcatum in Japan
title_short The Relationship between Mating System and Genetic Diversity in Diploid Sexual Populations of Cyrtomium falcatum in Japan
title_sort relationship between mating system and genetic diversity in diploid sexual populations of cyrtomium falcatum in japan
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5051678/
https://www.ncbi.nlm.nih.gov/pubmed/27706257
http://dx.doi.org/10.1371/journal.pone.0163683
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