Distinct combinations of variant ionotropic glutamate receptors mediate thermosensation and hygrosensation in Drosophila

Ionotropic Receptors (IRs) are a large subfamily of variant ionotropic glutamate receptors present across Protostomia. While these receptors are most extensively studied for their roles in chemosensory detection, recent work has implicated two family members, IR21a and IR25a, in thermosensation in D...

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Autores principales: Knecht, Zachary A, Silbering, Ana F, Ni, Lina, Klein, Mason, Budelli, Gonzalo, Bell, Rati, Abuin, Liliane, Ferrer, Anggie J, Samuel, Aravinthan DT, Benton, Richard, Garrity, Paul A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5052030/
https://www.ncbi.nlm.nih.gov/pubmed/27656904
http://dx.doi.org/10.7554/eLife.17879
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author Knecht, Zachary A
Silbering, Ana F
Ni, Lina
Klein, Mason
Budelli, Gonzalo
Bell, Rati
Abuin, Liliane
Ferrer, Anggie J
Samuel, Aravinthan DT
Benton, Richard
Garrity, Paul A
author_facet Knecht, Zachary A
Silbering, Ana F
Ni, Lina
Klein, Mason
Budelli, Gonzalo
Bell, Rati
Abuin, Liliane
Ferrer, Anggie J
Samuel, Aravinthan DT
Benton, Richard
Garrity, Paul A
author_sort Knecht, Zachary A
collection PubMed
description Ionotropic Receptors (IRs) are a large subfamily of variant ionotropic glutamate receptors present across Protostomia. While these receptors are most extensively studied for their roles in chemosensory detection, recent work has implicated two family members, IR21a and IR25a, in thermosensation in Drosophila. Here we characterize one of the most evolutionarily deeply conserved receptors, IR93a, and show that it is co-expressed and functions with IR21a and IR25a to mediate physiological and behavioral responses to cool temperatures. IR93a is also co-expressed with IR25a and a distinct receptor, IR40a, in a discrete population of sensory neurons in the sacculus, a multi-chambered pocket within the antenna. We demonstrate that this combination of receptors is required for neuronal responses to dry air and behavioral discrimination of humidity differences. Our results identify IR93a as a common component of molecularly and cellularly distinct IR pathways important for thermosensation and hygrosensation in insects. DOI: http://dx.doi.org/10.7554/eLife.17879.001
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spelling pubmed-50520302016-10-06 Distinct combinations of variant ionotropic glutamate receptors mediate thermosensation and hygrosensation in Drosophila Knecht, Zachary A Silbering, Ana F Ni, Lina Klein, Mason Budelli, Gonzalo Bell, Rati Abuin, Liliane Ferrer, Anggie J Samuel, Aravinthan DT Benton, Richard Garrity, Paul A eLife Neuroscience Ionotropic Receptors (IRs) are a large subfamily of variant ionotropic glutamate receptors present across Protostomia. While these receptors are most extensively studied for their roles in chemosensory detection, recent work has implicated two family members, IR21a and IR25a, in thermosensation in Drosophila. Here we characterize one of the most evolutionarily deeply conserved receptors, IR93a, and show that it is co-expressed and functions with IR21a and IR25a to mediate physiological and behavioral responses to cool temperatures. IR93a is also co-expressed with IR25a and a distinct receptor, IR40a, in a discrete population of sensory neurons in the sacculus, a multi-chambered pocket within the antenna. We demonstrate that this combination of receptors is required for neuronal responses to dry air and behavioral discrimination of humidity differences. Our results identify IR93a as a common component of molecularly and cellularly distinct IR pathways important for thermosensation and hygrosensation in insects. DOI: http://dx.doi.org/10.7554/eLife.17879.001 eLife Sciences Publications, Ltd 2016-09-22 /pmc/articles/PMC5052030/ /pubmed/27656904 http://dx.doi.org/10.7554/eLife.17879 Text en © 2016, Knecht et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Neuroscience
Knecht, Zachary A
Silbering, Ana F
Ni, Lina
Klein, Mason
Budelli, Gonzalo
Bell, Rati
Abuin, Liliane
Ferrer, Anggie J
Samuel, Aravinthan DT
Benton, Richard
Garrity, Paul A
Distinct combinations of variant ionotropic glutamate receptors mediate thermosensation and hygrosensation in Drosophila
title Distinct combinations of variant ionotropic glutamate receptors mediate thermosensation and hygrosensation in Drosophila
title_full Distinct combinations of variant ionotropic glutamate receptors mediate thermosensation and hygrosensation in Drosophila
title_fullStr Distinct combinations of variant ionotropic glutamate receptors mediate thermosensation and hygrosensation in Drosophila
title_full_unstemmed Distinct combinations of variant ionotropic glutamate receptors mediate thermosensation and hygrosensation in Drosophila
title_short Distinct combinations of variant ionotropic glutamate receptors mediate thermosensation and hygrosensation in Drosophila
title_sort distinct combinations of variant ionotropic glutamate receptors mediate thermosensation and hygrosensation in drosophila
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5052030/
https://www.ncbi.nlm.nih.gov/pubmed/27656904
http://dx.doi.org/10.7554/eLife.17879
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