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Use of homologous recombination in yeast to create chimeric bovine viral diarrhea virus cDNA clones

The open reading frame of a Brazilian bovine viral diarrhea virus (BVDV) strain, IBSP4ncp, was recombined with the untranslated regions of the reference NADL strain by homologous recombination in Saccharomyces cerevisiae, resulting in chimeric full-length cDNA clones of BVDV (chi-NADL/IBSP4ncp#2 and...

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Detalles Bibliográficos
Autores principales: Arenhart, Sandra, Silva, José Valter Joaquim, Flores, Eduardo Furtado, Weiblen, Rudi, Gil, Laura Helena Vega Gonzales
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5052387/
https://www.ncbi.nlm.nih.gov/pubmed/27522929
http://dx.doi.org/10.1016/j.bjm.2016.07.022