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Molecular determinants for the strictly compartmentalized expression of kainate receptors in CA3 pyramidal cells
Distinct subtypes of ionotropic glutamate receptors can segregate to specific synaptic inputs in a given neuron. Using functional mapping by focal glutamate uncaging in CA3 pyramidal cells (PCs), we observe that kainate receptors (KARs) are strictly confined to the postsynaptic elements of mossy fib...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5052629/ https://www.ncbi.nlm.nih.gov/pubmed/27669960 http://dx.doi.org/10.1038/ncomms12738 |
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author | Fièvre, Sabine Carta, Mario Chamma, Ingrid Labrousse, Virginie Thoumine, Olivier Mulle, Christophe |
author_facet | Fièvre, Sabine Carta, Mario Chamma, Ingrid Labrousse, Virginie Thoumine, Olivier Mulle, Christophe |
author_sort | Fièvre, Sabine |
collection | PubMed |
description | Distinct subtypes of ionotropic glutamate receptors can segregate to specific synaptic inputs in a given neuron. Using functional mapping by focal glutamate uncaging in CA3 pyramidal cells (PCs), we observe that kainate receptors (KARs) are strictly confined to the postsynaptic elements of mossy fibre (mf) synapses and excluded from other glutamatergic inputs and from extrasynaptic compartments. By molecular replacement in organotypic slices from GluK2 knockout mice, we show that the faithful rescue of KAR segregation at mf-CA3 synapses critically depends on the amount of GluK2a cDNA transfected and on a sequence in the GluK2a C-terminal domain responsible for interaction with N-cadherin. Targeted deletion of N-cadherin in CA3 PCs greatly reduces KAR content in thorny excrescences and KAR-EPSCs at mf-CA3 synapses. Hence, multiple mechanisms combine to confine KARs at mf-CA3 synapses, including a stringent control of the amount of GluK2 subunit in CA3 PCs and the recruitment/stabilization of KARs by N-cadherins. |
format | Online Article Text |
id | pubmed-5052629 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-50526292016-10-21 Molecular determinants for the strictly compartmentalized expression of kainate receptors in CA3 pyramidal cells Fièvre, Sabine Carta, Mario Chamma, Ingrid Labrousse, Virginie Thoumine, Olivier Mulle, Christophe Nat Commun Article Distinct subtypes of ionotropic glutamate receptors can segregate to specific synaptic inputs in a given neuron. Using functional mapping by focal glutamate uncaging in CA3 pyramidal cells (PCs), we observe that kainate receptors (KARs) are strictly confined to the postsynaptic elements of mossy fibre (mf) synapses and excluded from other glutamatergic inputs and from extrasynaptic compartments. By molecular replacement in organotypic slices from GluK2 knockout mice, we show that the faithful rescue of KAR segregation at mf-CA3 synapses critically depends on the amount of GluK2a cDNA transfected and on a sequence in the GluK2a C-terminal domain responsible for interaction with N-cadherin. Targeted deletion of N-cadherin in CA3 PCs greatly reduces KAR content in thorny excrescences and KAR-EPSCs at mf-CA3 synapses. Hence, multiple mechanisms combine to confine KARs at mf-CA3 synapses, including a stringent control of the amount of GluK2 subunit in CA3 PCs and the recruitment/stabilization of KARs by N-cadherins. Nature Publishing Group 2016-09-27 /pmc/articles/PMC5052629/ /pubmed/27669960 http://dx.doi.org/10.1038/ncomms12738 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Fièvre, Sabine Carta, Mario Chamma, Ingrid Labrousse, Virginie Thoumine, Olivier Mulle, Christophe Molecular determinants for the strictly compartmentalized expression of kainate receptors in CA3 pyramidal cells |
title | Molecular determinants for the strictly compartmentalized expression of kainate receptors in CA3 pyramidal cells |
title_full | Molecular determinants for the strictly compartmentalized expression of kainate receptors in CA3 pyramidal cells |
title_fullStr | Molecular determinants for the strictly compartmentalized expression of kainate receptors in CA3 pyramidal cells |
title_full_unstemmed | Molecular determinants for the strictly compartmentalized expression of kainate receptors in CA3 pyramidal cells |
title_short | Molecular determinants for the strictly compartmentalized expression of kainate receptors in CA3 pyramidal cells |
title_sort | molecular determinants for the strictly compartmentalized expression of kainate receptors in ca3 pyramidal cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5052629/ https://www.ncbi.nlm.nih.gov/pubmed/27669960 http://dx.doi.org/10.1038/ncomms12738 |
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