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Silencing of SOX12 by shRNA suppresses migration, invasion and proliferation of breast cancer cells

Sex determining region Y-box protein 12 (SOX12) is essential for embryonic development and cell-fate determination. The role of SOX12 in tumorigenesis of breast cancer is not well-understood. Here, we found that SOX12 mRNA expression was up-regulated in human breast cancer tissues. To clarify the ro...

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Detalles Bibliográficos
Autores principales: Ding, Hanzhi, Quan, Hong, Yan, Weiguo, Han, Jing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5052717/
https://www.ncbi.nlm.nih.gov/pubmed/27582508
http://dx.doi.org/10.1042/BSR20160053
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author Ding, Hanzhi
Quan, Hong
Yan, Weiguo
Han, Jing
author_facet Ding, Hanzhi
Quan, Hong
Yan, Weiguo
Han, Jing
author_sort Ding, Hanzhi
collection PubMed
description Sex determining region Y-box protein 12 (SOX12) is essential for embryonic development and cell-fate determination. The role of SOX12 in tumorigenesis of breast cancer is not well-understood. Here, we found that SOX12 mRNA expression was up-regulated in human breast cancer tissues. To clarify the roles of SOX12 in breast cancer, we used lentiviral shRNAs to suppress its expression in two breast cancer cells with relatively higher expression of SOX12 (BT474 and MCF-7). Our findings strongly suggested that SOX12 was critical for cell migration and invasion of breast cancer cells. We found that silencing of SOX12 significantly decreased the mRNA and protein levels of MMP9 and Twist, while notably increased E-cadherin. Moreover, SOX12 knockdown significantly inhibited the proliferation of breast cancer cells in vitro and the growth of xenograft tumours in vivo. Flow cytometry analysis revealed that breast cancer cells with SOX12 knockdown showed cell cycle arrest and decreased mRNA and protein levels of proliferating cell nuclear antigen (PCNA), CDK2 and Cyclin D1. Taken together, SOX12 plays an important role in growth inhibition through cell-cycle arrest, as well as migration and invasion of breast cancer cells.
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spelling pubmed-50527172016-10-20 Silencing of SOX12 by shRNA suppresses migration, invasion and proliferation of breast cancer cells Ding, Hanzhi Quan, Hong Yan, Weiguo Han, Jing Biosci Rep Original Papers Sex determining region Y-box protein 12 (SOX12) is essential for embryonic development and cell-fate determination. The role of SOX12 in tumorigenesis of breast cancer is not well-understood. Here, we found that SOX12 mRNA expression was up-regulated in human breast cancer tissues. To clarify the roles of SOX12 in breast cancer, we used lentiviral shRNAs to suppress its expression in two breast cancer cells with relatively higher expression of SOX12 (BT474 and MCF-7). Our findings strongly suggested that SOX12 was critical for cell migration and invasion of breast cancer cells. We found that silencing of SOX12 significantly decreased the mRNA and protein levels of MMP9 and Twist, while notably increased E-cadherin. Moreover, SOX12 knockdown significantly inhibited the proliferation of breast cancer cells in vitro and the growth of xenograft tumours in vivo. Flow cytometry analysis revealed that breast cancer cells with SOX12 knockdown showed cell cycle arrest and decreased mRNA and protein levels of proliferating cell nuclear antigen (PCNA), CDK2 and Cyclin D1. Taken together, SOX12 plays an important role in growth inhibition through cell-cycle arrest, as well as migration and invasion of breast cancer cells. Portland Press Ltd. 2016-10-06 /pmc/articles/PMC5052717/ /pubmed/27582508 http://dx.doi.org/10.1042/BSR20160053 Text en © 2016 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution Licence 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Papers
Ding, Hanzhi
Quan, Hong
Yan, Weiguo
Han, Jing
Silencing of SOX12 by shRNA suppresses migration, invasion and proliferation of breast cancer cells
title Silencing of SOX12 by shRNA suppresses migration, invasion and proliferation of breast cancer cells
title_full Silencing of SOX12 by shRNA suppresses migration, invasion and proliferation of breast cancer cells
title_fullStr Silencing of SOX12 by shRNA suppresses migration, invasion and proliferation of breast cancer cells
title_full_unstemmed Silencing of SOX12 by shRNA suppresses migration, invasion and proliferation of breast cancer cells
title_short Silencing of SOX12 by shRNA suppresses migration, invasion and proliferation of breast cancer cells
title_sort silencing of sox12 by shrna suppresses migration, invasion and proliferation of breast cancer cells
topic Original Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5052717/
https://www.ncbi.nlm.nih.gov/pubmed/27582508
http://dx.doi.org/10.1042/BSR20160053
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