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Genetic, histochemical and biochemical studies on goat TSE cases from Cyprus
Scrapie and bovine spongiform encephalopathy (BSE) are transmissible spongiform encephalopathies (TSE’s) affecting sheep and goats. Susceptibility of goats to scrapie is influenced by polymorphisms of the prion protein gene (PRNP) of the host. Five polymorphisms are associated with reduced susceptib...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5053211/ https://www.ncbi.nlm.nih.gov/pubmed/27716411 http://dx.doi.org/10.1186/s13567-016-0379-0 |
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author | Niedermeyer, Susanne Eiden, Martin Toumazos, Pavlos Papasavva-Stylianou, Penelope Ioannou, Ioannis Sklaviadis, Theodoros Panagiotidis, Cynthia Langeveld, Jan Bossers, Alex Kuczius, Thorsten Kaatz, Martin Groschup, Martin H. Fast, Christine |
author_facet | Niedermeyer, Susanne Eiden, Martin Toumazos, Pavlos Papasavva-Stylianou, Penelope Ioannou, Ioannis Sklaviadis, Theodoros Panagiotidis, Cynthia Langeveld, Jan Bossers, Alex Kuczius, Thorsten Kaatz, Martin Groschup, Martin H. Fast, Christine |
author_sort | Niedermeyer, Susanne |
collection | PubMed |
description | Scrapie and bovine spongiform encephalopathy (BSE) are transmissible spongiform encephalopathies (TSE’s) affecting sheep and goats. Susceptibility of goats to scrapie is influenced by polymorphisms of the prion protein gene (PRNP) of the host. Five polymorphisms are associated with reduced susceptibility to TSE’s. In the study presented here caprine samples from a scrapie eradication program on Cyprus were genotyped and further characterized using BioRad TeSeE rapid test, histological, immunohistochemical and biochemical methods. In total 42 goats from 20 flocks were necropsied from which 25 goats showed a positive result in the rapid test, a spongiform encephalopathy and an accumulation of pathological prion protein (PrP(Sc)) in the obex. PrP(Sc) deposits were demonstrated in the placenta, peripheral nervous and lymphoreticular system. Two animals showed PrP(Sc)-accumulations in peripheral tissues only. By discriminatory immunoblots a scrapie infection could be confirmed for all cases. Nevertheless, slight deviations in the glycosylation pattern might indicate the presence of different scrapie strains. Furthermore scrapie samples from goats in the current study demonstrated less long term resistance to proteinase K than ovine or caprine BSE control samples. Reduced scrapie susceptibility according to the PRNP genotype was demonstrated (Fishers Exact test, p < 0.05) for the goats with at least one polymorphism (p = 0.023) at the six codons examined and in particular for those with polymorphisms at codon 146 (p = 0.016). This work characterizes scrapie in goats having implications for breeding and surveillance strategies. |
format | Online Article Text |
id | pubmed-5053211 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-50532112016-10-18 Genetic, histochemical and biochemical studies on goat TSE cases from Cyprus Niedermeyer, Susanne Eiden, Martin Toumazos, Pavlos Papasavva-Stylianou, Penelope Ioannou, Ioannis Sklaviadis, Theodoros Panagiotidis, Cynthia Langeveld, Jan Bossers, Alex Kuczius, Thorsten Kaatz, Martin Groschup, Martin H. Fast, Christine Vet Res Research Article Scrapie and bovine spongiform encephalopathy (BSE) are transmissible spongiform encephalopathies (TSE’s) affecting sheep and goats. Susceptibility of goats to scrapie is influenced by polymorphisms of the prion protein gene (PRNP) of the host. Five polymorphisms are associated with reduced susceptibility to TSE’s. In the study presented here caprine samples from a scrapie eradication program on Cyprus were genotyped and further characterized using BioRad TeSeE rapid test, histological, immunohistochemical and biochemical methods. In total 42 goats from 20 flocks were necropsied from which 25 goats showed a positive result in the rapid test, a spongiform encephalopathy and an accumulation of pathological prion protein (PrP(Sc)) in the obex. PrP(Sc) deposits were demonstrated in the placenta, peripheral nervous and lymphoreticular system. Two animals showed PrP(Sc)-accumulations in peripheral tissues only. By discriminatory immunoblots a scrapie infection could be confirmed for all cases. Nevertheless, slight deviations in the glycosylation pattern might indicate the presence of different scrapie strains. Furthermore scrapie samples from goats in the current study demonstrated less long term resistance to proteinase K than ovine or caprine BSE control samples. Reduced scrapie susceptibility according to the PRNP genotype was demonstrated (Fishers Exact test, p < 0.05) for the goats with at least one polymorphism (p = 0.023) at the six codons examined and in particular for those with polymorphisms at codon 146 (p = 0.016). This work characterizes scrapie in goats having implications for breeding and surveillance strategies. BioMed Central 2016-10-06 2016 /pmc/articles/PMC5053211/ /pubmed/27716411 http://dx.doi.org/10.1186/s13567-016-0379-0 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Niedermeyer, Susanne Eiden, Martin Toumazos, Pavlos Papasavva-Stylianou, Penelope Ioannou, Ioannis Sklaviadis, Theodoros Panagiotidis, Cynthia Langeveld, Jan Bossers, Alex Kuczius, Thorsten Kaatz, Martin Groschup, Martin H. Fast, Christine Genetic, histochemical and biochemical studies on goat TSE cases from Cyprus |
title | Genetic, histochemical and biochemical studies on goat TSE cases from Cyprus |
title_full | Genetic, histochemical and biochemical studies on goat TSE cases from Cyprus |
title_fullStr | Genetic, histochemical and biochemical studies on goat TSE cases from Cyprus |
title_full_unstemmed | Genetic, histochemical and biochemical studies on goat TSE cases from Cyprus |
title_short | Genetic, histochemical and biochemical studies on goat TSE cases from Cyprus |
title_sort | genetic, histochemical and biochemical studies on goat tse cases from cyprus |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5053211/ https://www.ncbi.nlm.nih.gov/pubmed/27716411 http://dx.doi.org/10.1186/s13567-016-0379-0 |
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