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Meet the neighbors: Mapping local protein interactomes by proximity‐dependent labeling with BioID

Proximity‐dependent biotin identification (BioID) is a recently developed method that allows the identification of proteins in the close vicinity of a protein of interest in living cells. BioID relies on fusion of the protein of interest with a mutant form of the biotin ligase enzyme BirA (BirA*) th...

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Detalles Bibliográficos
Autores principales: Varnaitė, Renata, MacNeill, Stuart A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5053326/
https://www.ncbi.nlm.nih.gov/pubmed/27329485
http://dx.doi.org/10.1002/pmic.201600123