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Flow cytometric analysis of inflammatory and resident myeloid populations in mouse ocular inflammatory models

Myeloid cells make a pivotal contribution to tissue homeostasis during inflammation. Both tissue-specific resident populations and infiltrating myeloid cells can cause tissue injury through aberrant activation and/or dysregulated activity. Reliable identification and quantification of myeloid cells...

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Detalles Bibliográficos
Autores principales: Liyanage, Sidath E., Gardner, Peter J., Ribeiro, Joana, Cristante, Enrico, Sampson, Robert D., Luhmann, Ulrich F.O., Ali, Robin R., Bainbridge, James W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academic Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5053376/
https://www.ncbi.nlm.nih.gov/pubmed/27544307
http://dx.doi.org/10.1016/j.exer.2016.08.007
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author Liyanage, Sidath E.
Gardner, Peter J.
Ribeiro, Joana
Cristante, Enrico
Sampson, Robert D.
Luhmann, Ulrich F.O.
Ali, Robin R.
Bainbridge, James W.
author_facet Liyanage, Sidath E.
Gardner, Peter J.
Ribeiro, Joana
Cristante, Enrico
Sampson, Robert D.
Luhmann, Ulrich F.O.
Ali, Robin R.
Bainbridge, James W.
author_sort Liyanage, Sidath E.
collection PubMed
description Myeloid cells make a pivotal contribution to tissue homeostasis during inflammation. Both tissue-specific resident populations and infiltrating myeloid cells can cause tissue injury through aberrant activation and/or dysregulated activity. Reliable identification and quantification of myeloid cells within diseased tissues is important to understand pathological inflammatory processes. Flow cytometry is a valuable technique for leukocyte analysis, but a standardized flow cytometric method for myeloid cell populations in the eye is lacking. Here, we validate a reproducible flow cytometry gating approach to characterize myeloid cells in several commonly used models of ocular inflammation. We profile and quantify myeloid subsets across these models, and highlight the value of this strategy in identifying phenotypic differences using Ccr2-deficient mice. This method will aid standardization in the field and facilitate future investigations into the roles of myeloid cells during ocular inflammation.
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spelling pubmed-50533762016-10-12 Flow cytometric analysis of inflammatory and resident myeloid populations in mouse ocular inflammatory models Liyanage, Sidath E. Gardner, Peter J. Ribeiro, Joana Cristante, Enrico Sampson, Robert D. Luhmann, Ulrich F.O. Ali, Robin R. Bainbridge, James W. Exp Eye Res Methods in Eye Research Myeloid cells make a pivotal contribution to tissue homeostasis during inflammation. Both tissue-specific resident populations and infiltrating myeloid cells can cause tissue injury through aberrant activation and/or dysregulated activity. Reliable identification and quantification of myeloid cells within diseased tissues is important to understand pathological inflammatory processes. Flow cytometry is a valuable technique for leukocyte analysis, but a standardized flow cytometric method for myeloid cell populations in the eye is lacking. Here, we validate a reproducible flow cytometry gating approach to characterize myeloid cells in several commonly used models of ocular inflammation. We profile and quantify myeloid subsets across these models, and highlight the value of this strategy in identifying phenotypic differences using Ccr2-deficient mice. This method will aid standardization in the field and facilitate future investigations into the roles of myeloid cells during ocular inflammation. Academic Press 2016-10 /pmc/articles/PMC5053376/ /pubmed/27544307 http://dx.doi.org/10.1016/j.exer.2016.08.007 Text en © 2016 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Methods in Eye Research
Liyanage, Sidath E.
Gardner, Peter J.
Ribeiro, Joana
Cristante, Enrico
Sampson, Robert D.
Luhmann, Ulrich F.O.
Ali, Robin R.
Bainbridge, James W.
Flow cytometric analysis of inflammatory and resident myeloid populations in mouse ocular inflammatory models
title Flow cytometric analysis of inflammatory and resident myeloid populations in mouse ocular inflammatory models
title_full Flow cytometric analysis of inflammatory and resident myeloid populations in mouse ocular inflammatory models
title_fullStr Flow cytometric analysis of inflammatory and resident myeloid populations in mouse ocular inflammatory models
title_full_unstemmed Flow cytometric analysis of inflammatory and resident myeloid populations in mouse ocular inflammatory models
title_short Flow cytometric analysis of inflammatory and resident myeloid populations in mouse ocular inflammatory models
title_sort flow cytometric analysis of inflammatory and resident myeloid populations in mouse ocular inflammatory models
topic Methods in Eye Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5053376/
https://www.ncbi.nlm.nih.gov/pubmed/27544307
http://dx.doi.org/10.1016/j.exer.2016.08.007
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