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Mitophagy in TGEV infection counteracts oxidative stress and apoptosis

The intestinal epithelial cells contain a large number of mitochondria for persisting absorption and barrier function. Selective autophagy of mitochondria (mitophagy) plays an important role in the quality control of mitochondria and maintenance of cell homeostasis. Transmissible gastroenteritis vir...

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Autores principales: Zhu, Liqi, Mou, Chunxiao, Yang, Xing, Lin, Jian, Yang, Qian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5053637/
https://www.ncbi.nlm.nih.gov/pubmed/27027356
http://dx.doi.org/10.18632/oncotarget.8345
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author Zhu, Liqi
Mou, Chunxiao
Yang, Xing
Lin, Jian
Yang, Qian
author_facet Zhu, Liqi
Mou, Chunxiao
Yang, Xing
Lin, Jian
Yang, Qian
author_sort Zhu, Liqi
collection PubMed
description The intestinal epithelial cells contain a large number of mitochondria for persisting absorption and barrier function. Selective autophagy of mitochondria (mitophagy) plays an important role in the quality control of mitochondria and maintenance of cell homeostasis. Transmissible gastroenteritis virus (TGEV) is a porcine enteropathogenic coronavirus which induces malabsorption and lethal watery diarrhea in suckling piglets. The role of mitophagy in the pathological changes caused by TGEV infection is unclear. Here, we report that TGEV induces mitophagy to suppress oxidative stress and apoptosis induced by viral infection in porcine epithelial cells (IPEC-J2). We observe that TGEV infection induce mitochondrial injury, abnormal morphology, complete mitophagy, and without obvious apoptosis after TGEV infection. Meanwhile, TGEV also induces DJ-1 and some antioxidant genes upregulation to suppress oxidative stress induced by viral infection. Furthermore, silencing DJ-1 inhibit mitophagy and increase apoptosis after TGEV infection. In addition, we demonstrate for the first time that viral nucleocapsid protein (N) is located in mitochondria and mitophagosome during virus infection or be expressed alone. Those results provide a novel perspective for further improvement of prevention and treatment in TGEV infection. These results suggest that TGEV infection induce mitophagy to promote cell survival and possibly viral infection.
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spelling pubmed-50536372016-10-12 Mitophagy in TGEV infection counteracts oxidative stress and apoptosis Zhu, Liqi Mou, Chunxiao Yang, Xing Lin, Jian Yang, Qian Oncotarget Research Paper The intestinal epithelial cells contain a large number of mitochondria for persisting absorption and barrier function. Selective autophagy of mitochondria (mitophagy) plays an important role in the quality control of mitochondria and maintenance of cell homeostasis. Transmissible gastroenteritis virus (TGEV) is a porcine enteropathogenic coronavirus which induces malabsorption and lethal watery diarrhea in suckling piglets. The role of mitophagy in the pathological changes caused by TGEV infection is unclear. Here, we report that TGEV induces mitophagy to suppress oxidative stress and apoptosis induced by viral infection in porcine epithelial cells (IPEC-J2). We observe that TGEV infection induce mitochondrial injury, abnormal morphology, complete mitophagy, and without obvious apoptosis after TGEV infection. Meanwhile, TGEV also induces DJ-1 and some antioxidant genes upregulation to suppress oxidative stress induced by viral infection. Furthermore, silencing DJ-1 inhibit mitophagy and increase apoptosis after TGEV infection. In addition, we demonstrate for the first time that viral nucleocapsid protein (N) is located in mitochondria and mitophagosome during virus infection or be expressed alone. Those results provide a novel perspective for further improvement of prevention and treatment in TGEV infection. These results suggest that TGEV infection induce mitophagy to promote cell survival and possibly viral infection. Impact Journals LLC 2016-03-24 /pmc/articles/PMC5053637/ /pubmed/27027356 http://dx.doi.org/10.18632/oncotarget.8345 Text en Copyright: © 2016 Zhu et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Zhu, Liqi
Mou, Chunxiao
Yang, Xing
Lin, Jian
Yang, Qian
Mitophagy in TGEV infection counteracts oxidative stress and apoptosis
title Mitophagy in TGEV infection counteracts oxidative stress and apoptosis
title_full Mitophagy in TGEV infection counteracts oxidative stress and apoptosis
title_fullStr Mitophagy in TGEV infection counteracts oxidative stress and apoptosis
title_full_unstemmed Mitophagy in TGEV infection counteracts oxidative stress and apoptosis
title_short Mitophagy in TGEV infection counteracts oxidative stress and apoptosis
title_sort mitophagy in tgev infection counteracts oxidative stress and apoptosis
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5053637/
https://www.ncbi.nlm.nih.gov/pubmed/27027356
http://dx.doi.org/10.18632/oncotarget.8345
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