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Activation of epithelial proliferation induced by Eimeria acervulina infection in the duodenum may be associated with cholesterol metabolism

Cell proliferation in the intestine is commonly occurred during infection and inflammation to replace damaged enterocytes, and cholesterol as an essential constituent of cell membrane, is required for cell proliferation and growth. Here we found that coccidium-challenged (CC) chickens showed severe...

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Autores principales: Sun, Lili, Dong, Haibo, Zhang, Zhenchao, Liu, Jie, Hu, Yun, Ni, Yingdong, Grossmann, Roland, Zhao, Ruqian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5053676/
https://www.ncbi.nlm.nih.gov/pubmed/27050279
http://dx.doi.org/10.18632/oncotarget.8490
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author Sun, Lili
Dong, Haibo
Zhang, Zhenchao
Liu, Jie
Hu, Yun
Ni, Yingdong
Grossmann, Roland
Zhao, Ruqian
author_facet Sun, Lili
Dong, Haibo
Zhang, Zhenchao
Liu, Jie
Hu, Yun
Ni, Yingdong
Grossmann, Roland
Zhao, Ruqian
author_sort Sun, Lili
collection PubMed
description Cell proliferation in the intestine is commonly occurred during infection and inflammation to replace damaged enterocytes, and cholesterol as an essential constituent of cell membrane, is required for cell proliferation and growth. Here we found that coccidium-challenged (CC) chickens showed severe damages in intestinal structure, a significant increase of cell proliferation, and an activation of genes expression involved in the innate immune response. Compared to control (CON), CC chickens showed a marked decrease of cholesterol (Tch) level in the circulating system, but a significant increase in local duodenum epithelium. Increase of LDLR protein combined with a significant decrease of CYP27A1 protein expression in duodenum epithelium may contribute to intestinal cholesterol accumulation in CC chickens. Moreover, we found miRNAs targeting to CYP27A1 gene participating in post-transcriptional regulation. Hence, these results provide a new insight for the intervention of epithelial proliferation and cholesterol metabolism in the gastrointestinal tracts.
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spelling pubmed-50536762016-10-12 Activation of epithelial proliferation induced by Eimeria acervulina infection in the duodenum may be associated with cholesterol metabolism Sun, Lili Dong, Haibo Zhang, Zhenchao Liu, Jie Hu, Yun Ni, Yingdong Grossmann, Roland Zhao, Ruqian Oncotarget Research Paper Cell proliferation in the intestine is commonly occurred during infection and inflammation to replace damaged enterocytes, and cholesterol as an essential constituent of cell membrane, is required for cell proliferation and growth. Here we found that coccidium-challenged (CC) chickens showed severe damages in intestinal structure, a significant increase of cell proliferation, and an activation of genes expression involved in the innate immune response. Compared to control (CON), CC chickens showed a marked decrease of cholesterol (Tch) level in the circulating system, but a significant increase in local duodenum epithelium. Increase of LDLR protein combined with a significant decrease of CYP27A1 protein expression in duodenum epithelium may contribute to intestinal cholesterol accumulation in CC chickens. Moreover, we found miRNAs targeting to CYP27A1 gene participating in post-transcriptional regulation. Hence, these results provide a new insight for the intervention of epithelial proliferation and cholesterol metabolism in the gastrointestinal tracts. Impact Journals LLC 2016-03-30 /pmc/articles/PMC5053676/ /pubmed/27050279 http://dx.doi.org/10.18632/oncotarget.8490 Text en Copyright: © 2016 Sun et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Sun, Lili
Dong, Haibo
Zhang, Zhenchao
Liu, Jie
Hu, Yun
Ni, Yingdong
Grossmann, Roland
Zhao, Ruqian
Activation of epithelial proliferation induced by Eimeria acervulina infection in the duodenum may be associated with cholesterol metabolism
title Activation of epithelial proliferation induced by Eimeria acervulina infection in the duodenum may be associated with cholesterol metabolism
title_full Activation of epithelial proliferation induced by Eimeria acervulina infection in the duodenum may be associated with cholesterol metabolism
title_fullStr Activation of epithelial proliferation induced by Eimeria acervulina infection in the duodenum may be associated with cholesterol metabolism
title_full_unstemmed Activation of epithelial proliferation induced by Eimeria acervulina infection in the duodenum may be associated with cholesterol metabolism
title_short Activation of epithelial proliferation induced by Eimeria acervulina infection in the duodenum may be associated with cholesterol metabolism
title_sort activation of epithelial proliferation induced by eimeria acervulina infection in the duodenum may be associated with cholesterol metabolism
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5053676/
https://www.ncbi.nlm.nih.gov/pubmed/27050279
http://dx.doi.org/10.18632/oncotarget.8490
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