Development of a blocking ELISA for detection of Mycoplasma hyopneumoniae infection based on a monoclonal antibody against protein P65

Mycoplasma hyopneumoniae causes porcine enzootic pneumonia, an economically important disease of swine. A more sensitive and reliable method for detection of serum antibodies is needed for epidemiological investigations and to evaluate the effect of immunization. We expressed the M. hyopneumoniae pr...

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Autores principales: LIU, Maojun, DU, Gaimei, ZHANG, Yue, WU, Yuzi, WANG, Haiyan, LI, Bin, BAI, Yun, FENG, Zhixin, XIONG, Qiyan, BAI, Fangfang, BROWNING, Glenn F, SHAO, Guoqing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2016
Materias:
Immunology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5053934/
https://www.ncbi.nlm.nih.gov/pubmed/27075114
http://dx.doi.org/10.1292/jvms.15-0438
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author LIU, Maojun
DU, Gaimei
ZHANG, Yue
WU, Yuzi
WANG, Haiyan
LI, Bin
BAI, Yun
FENG, Zhixin
XIONG, Qiyan
BAI, Fangfang
BROWNING, Glenn F
SHAO, Guoqing
author_facet LIU, Maojun
DU, Gaimei
ZHANG, Yue
WU, Yuzi
WANG, Haiyan
LI, Bin
BAI, Yun
FENG, Zhixin
XIONG, Qiyan
BAI, Fangfang
BROWNING, Glenn F
SHAO, Guoqing
author_sort LIU, Maojun
collection PubMed
description Mycoplasma hyopneumoniae causes porcine enzootic pneumonia, an economically important disease of swine. A more sensitive and reliable method for detection of serum antibodies is needed for epidemiological investigations and to evaluate the effect of immunization. We expressed the M. hyopneumoniae protein P65 in Escherichia coli and produced a monoclonal antibody (mAb) that bound specifically to recombinant P65. Using this mAb, a blocking enzyme linked immunosorbent assay (ELISA) was developed. The blocking ELISA had similar specificity to and sensitivity with the commercial ELISA produced by IDEXX. Thus, this blocking ELISA is a useful test for serological confirmation of M. hyopneumoniae infection.
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spelling pubmed-50539342016-10-07 Development of a blocking ELISA for detection of Mycoplasma hyopneumoniae infection based on a monoclonal antibody against protein P65 LIU, Maojun DU, Gaimei ZHANG, Yue WU, Yuzi WANG, Haiyan LI, Bin BAI, Yun FENG, Zhixin XIONG, Qiyan BAI, Fangfang BROWNING, Glenn F SHAO, Guoqing J Vet Med Sci Immunology Mycoplasma hyopneumoniae causes porcine enzootic pneumonia, an economically important disease of swine. A more sensitive and reliable method for detection of serum antibodies is needed for epidemiological investigations and to evaluate the effect of immunization. We expressed the M. hyopneumoniae protein P65 in Escherichia coli and produced a monoclonal antibody (mAb) that bound specifically to recombinant P65. Using this mAb, a blocking enzyme linked immunosorbent assay (ELISA) was developed. The blocking ELISA had similar specificity to and sensitivity with the commercial ELISA produced by IDEXX. Thus, this blocking ELISA is a useful test for serological confirmation of M. hyopneumoniae infection. The Japanese Society of Veterinary Science 2016-04-14 2016-08 /pmc/articles/PMC5053934/ /pubmed/27075114 http://dx.doi.org/10.1292/jvms.15-0438 Text en ©2016 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Immunology
LIU, Maojun
DU, Gaimei
ZHANG, Yue
WU, Yuzi
WANG, Haiyan
LI, Bin
BAI, Yun
FENG, Zhixin
XIONG, Qiyan
BAI, Fangfang
BROWNING, Glenn F
SHAO, Guoqing
Development of a blocking ELISA for detection of Mycoplasma hyopneumoniae infection based on a monoclonal antibody against protein P65
title Development of a blocking ELISA for detection of Mycoplasma hyopneumoniae infection based on a monoclonal antibody against protein P65
title_full Development of a blocking ELISA for detection of Mycoplasma hyopneumoniae infection based on a monoclonal antibody against protein P65
title_fullStr Development of a blocking ELISA for detection of Mycoplasma hyopneumoniae infection based on a monoclonal antibody against protein P65
title_full_unstemmed Development of a blocking ELISA for detection of Mycoplasma hyopneumoniae infection based on a monoclonal antibody against protein P65
title_short Development of a blocking ELISA for detection of Mycoplasma hyopneumoniae infection based on a monoclonal antibody against protein P65
title_sort development of a blocking elisa for detection of mycoplasma hyopneumoniae infection based on a monoclonal antibody against protein p65
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5053934/
https://www.ncbi.nlm.nih.gov/pubmed/27075114
http://dx.doi.org/10.1292/jvms.15-0438
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