The use of MALDI-TOF mass spectrometry for rapid identification of Mannheimia haemolytica

Mannheimia haemolytica is the most important bacterial pathogen isolated from cases of Bovine Respiratory Disease (BRD). Routine identification of these bacteria is usually performed using phenotypic methods. Our study showed that MALDI-TOF MS is a reliable alternative to these methods. All of the s...

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Autores principales: PUCHALSKI, Andrzej, URBAN-CHMIEL, Renata, DEC, Marta, STĘGIERSKA, Diana, WERNICKI, Andrzej
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2016
Materias:
Bacteriology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5053938/
https://www.ncbi.nlm.nih.gov/pubmed/27109070
http://dx.doi.org/10.1292/jvms.16-0087
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author PUCHALSKI, Andrzej
URBAN-CHMIEL, Renata
DEC, Marta
STĘGIERSKA, Diana
WERNICKI, Andrzej
author_facet PUCHALSKI, Andrzej
URBAN-CHMIEL, Renata
DEC, Marta
STĘGIERSKA, Diana
WERNICKI, Andrzej
author_sort PUCHALSKI, Andrzej
collection PubMed
description Mannheimia haemolytica is the most important bacterial pathogen isolated from cases of Bovine Respiratory Disease (BRD). Routine identification of these bacteria is usually performed using phenotypic methods. Our study showed that MALDI-TOF MS is a reliable alternative to these methods. All of the strains analyzed were identified as M. haemolytica. The identification results were compared to those obtained using conventional methods commonly used in microbiological diagnostics, based on detection and analysis of biochemical properties of microorganisms. The degree of agreement between the two methods for identifying M. haemolytica was 100%.
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spelling pubmed-50539382016-10-07 The use of MALDI-TOF mass spectrometry for rapid identification of Mannheimia haemolytica PUCHALSKI, Andrzej URBAN-CHMIEL, Renata DEC, Marta STĘGIERSKA, Diana WERNICKI, Andrzej J Vet Med Sci Bacteriology Mannheimia haemolytica is the most important bacterial pathogen isolated from cases of Bovine Respiratory Disease (BRD). Routine identification of these bacteria is usually performed using phenotypic methods. Our study showed that MALDI-TOF MS is a reliable alternative to these methods. All of the strains analyzed were identified as M. haemolytica. The identification results were compared to those obtained using conventional methods commonly used in microbiological diagnostics, based on detection and analysis of biochemical properties of microorganisms. The degree of agreement between the two methods for identifying M. haemolytica was 100%. The Japanese Society of Veterinary Science 2016-04-24 2016-08 /pmc/articles/PMC5053938/ /pubmed/27109070 http://dx.doi.org/10.1292/jvms.16-0087 Text en ©2016 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Bacteriology
PUCHALSKI, Andrzej
URBAN-CHMIEL, Renata
DEC, Marta
STĘGIERSKA, Diana
WERNICKI, Andrzej
The use of MALDI-TOF mass spectrometry for rapid identification of Mannheimia haemolytica
title The use of MALDI-TOF mass spectrometry for rapid identification of Mannheimia haemolytica
title_full The use of MALDI-TOF mass spectrometry for rapid identification of Mannheimia haemolytica
title_fullStr The use of MALDI-TOF mass spectrometry for rapid identification of Mannheimia haemolytica
title_full_unstemmed The use of MALDI-TOF mass spectrometry for rapid identification of Mannheimia haemolytica
title_short The use of MALDI-TOF mass spectrometry for rapid identification of Mannheimia haemolytica
title_sort use of maldi-tof mass spectrometry for rapid identification of mannheimia haemolytica
topic Bacteriology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5053938/
https://www.ncbi.nlm.nih.gov/pubmed/27109070
http://dx.doi.org/10.1292/jvms.16-0087
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