An improved loop-mediated isothermal amplification assay for the detection of Mycoplasma bovis

We improved a loop-mediated isothermal amplification (LAMP) assay permitting sensitive and rapid Mycoplasma bovis detection. A total of 55 bacterial strains were examined in this study, including 33 M. bovis strains, 14 non-M. bovis mycoplasmas and eight non-mycoplasma bacterial strains. M. bovis wa...

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Autores principales: HIGA, Yumiko, UEMURA, Ryoko, YAMAZAKI, Wataru, GOTO, Shinya, GOTO, Yoshitaka, SUEYOSHI, Masuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2016
Materias:
Bacteriology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5053939/
https://www.ncbi.nlm.nih.gov/pubmed/27109067
http://dx.doi.org/10.1292/jvms.15-0459
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author HIGA, Yumiko
UEMURA, Ryoko
YAMAZAKI, Wataru
GOTO, Shinya
GOTO, Yoshitaka
SUEYOSHI, Masuo
author_facet HIGA, Yumiko
UEMURA, Ryoko
YAMAZAKI, Wataru
GOTO, Shinya
GOTO, Yoshitaka
SUEYOSHI, Masuo
author_sort HIGA, Yumiko
collection PubMed
description We improved a loop-mediated isothermal amplification (LAMP) assay permitting sensitive and rapid Mycoplasma bovis detection. A total of 55 bacterial strains were examined in this study, including 33 M. bovis strains, 14 non-M. bovis mycoplasmas and eight non-mycoplasma bacterial strains. M. bovis was successfully detected by the LAMP assay within 60 min without cross-reaction to any other bacteria. Furthermore, a total of 135 nasal swab samples were tested directly using our LAMP assays, the previously reported LAMP assay, conventional PCR assay without pre-culture and comparing standard culture methods. The improved LAMP assay showed sensitivity and specificity of 97.2% and 90.9%, respectively (with a kappa coefficient of 0.8231), and the sensitivity of our revised LAMP assay was increased compared to existing methods.
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spelling pubmed-50539392016-10-07 An improved loop-mediated isothermal amplification assay for the detection of Mycoplasma bovis HIGA, Yumiko UEMURA, Ryoko YAMAZAKI, Wataru GOTO, Shinya GOTO, Yoshitaka SUEYOSHI, Masuo J Vet Med Sci Bacteriology We improved a loop-mediated isothermal amplification (LAMP) assay permitting sensitive and rapid Mycoplasma bovis detection. A total of 55 bacterial strains were examined in this study, including 33 M. bovis strains, 14 non-M. bovis mycoplasmas and eight non-mycoplasma bacterial strains. M. bovis was successfully detected by the LAMP assay within 60 min without cross-reaction to any other bacteria. Furthermore, a total of 135 nasal swab samples were tested directly using our LAMP assays, the previously reported LAMP assay, conventional PCR assay without pre-culture and comparing standard culture methods. The improved LAMP assay showed sensitivity and specificity of 97.2% and 90.9%, respectively (with a kappa coefficient of 0.8231), and the sensitivity of our revised LAMP assay was increased compared to existing methods. The Japanese Society of Veterinary Science 2016-04-24 2016-08 /pmc/articles/PMC5053939/ /pubmed/27109067 http://dx.doi.org/10.1292/jvms.15-0459 Text en ©2016 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Bacteriology
HIGA, Yumiko
UEMURA, Ryoko
YAMAZAKI, Wataru
GOTO, Shinya
GOTO, Yoshitaka
SUEYOSHI, Masuo
An improved loop-mediated isothermal amplification assay for the detection of Mycoplasma bovis
title An improved loop-mediated isothermal amplification assay for the detection of Mycoplasma bovis
title_full An improved loop-mediated isothermal amplification assay for the detection of Mycoplasma bovis
title_fullStr An improved loop-mediated isothermal amplification assay for the detection of Mycoplasma bovis
title_full_unstemmed An improved loop-mediated isothermal amplification assay for the detection of Mycoplasma bovis
title_short An improved loop-mediated isothermal amplification assay for the detection of Mycoplasma bovis
title_sort improved loop-mediated isothermal amplification assay for the detection of mycoplasma bovis
topic Bacteriology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5053939/
https://www.ncbi.nlm.nih.gov/pubmed/27109067
http://dx.doi.org/10.1292/jvms.15-0459
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