Molecular cloning, phylogenetic analysis and heat shock response of Babesia gibsoni heat shock protein 90

The Babesia gibsoni heat shock protein 90 (BgHSP90) gene was cloned and sequenced. The length of the gene was 2,610 bp with two introns. This gene was amplified from cDNA corresponding to full length coding sequence (CDS) with an open reading frame of 2,148 bp. A phylogenetic analysis of the CDS of...

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Autores principales: YAMASAKI, Masahiro, TSUBOI, Yoshihiro, TANIYAMA, Yusuke, UCHIDA, Naohiro, SATO, Reeko, NAKAMURA, Kensuke, OHTA, Hiroshi, TAKIGUCHI, Mitsuyoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2016
Materias:
Parasitology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5053942/
https://www.ncbi.nlm.nih.gov/pubmed/27149891
http://dx.doi.org/10.1292/jvms.16-0027
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author YAMASAKI, Masahiro
TSUBOI, Yoshihiro
TANIYAMA, Yusuke
UCHIDA, Naohiro
SATO, Reeko
NAKAMURA, Kensuke
OHTA, Hiroshi
TAKIGUCHI, Mitsuyoshi
author_facet YAMASAKI, Masahiro
TSUBOI, Yoshihiro
TANIYAMA, Yusuke
UCHIDA, Naohiro
SATO, Reeko
NAKAMURA, Kensuke
OHTA, Hiroshi
TAKIGUCHI, Mitsuyoshi
author_sort YAMASAKI, Masahiro
collection PubMed
description The Babesia gibsoni heat shock protein 90 (BgHSP90) gene was cloned and sequenced. The length of the gene was 2,610 bp with two introns. This gene was amplified from cDNA corresponding to full length coding sequence (CDS) with an open reading frame of 2,148 bp. A phylogenetic analysis of the CDS of HSP90 gene showed that B. gibsoni was most closely related to B. bovis and Babesia sp. BQ1/Lintan and lies within a phylogenetic cluster of protozoa. Moreover, mRNA transcription profile for BgHSP90 exposed to high temperature were examined by quantitative real-time reverse transcription-polymerase chain reaction. BgHSP90 levels were elevated when the parasites were incubated at 43°C for 1 hr.
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spelling pubmed-50539422016-10-07 Molecular cloning, phylogenetic analysis and heat shock response of Babesia gibsoni heat shock protein 90 YAMASAKI, Masahiro TSUBOI, Yoshihiro TANIYAMA, Yusuke UCHIDA, Naohiro SATO, Reeko NAKAMURA, Kensuke OHTA, Hiroshi TAKIGUCHI, Mitsuyoshi J Vet Med Sci Parasitology The Babesia gibsoni heat shock protein 90 (BgHSP90) gene was cloned and sequenced. The length of the gene was 2,610 bp with two introns. This gene was amplified from cDNA corresponding to full length coding sequence (CDS) with an open reading frame of 2,148 bp. A phylogenetic analysis of the CDS of HSP90 gene showed that B. gibsoni was most closely related to B. bovis and Babesia sp. BQ1/Lintan and lies within a phylogenetic cluster of protozoa. Moreover, mRNA transcription profile for BgHSP90 exposed to high temperature were examined by quantitative real-time reverse transcription-polymerase chain reaction. BgHSP90 levels were elevated when the parasites were incubated at 43°C for 1 hr. The Japanese Society of Veterinary Science 2016-04-28 2016-08 /pmc/articles/PMC5053942/ /pubmed/27149891 http://dx.doi.org/10.1292/jvms.16-0027 Text en ©2016 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Parasitology
YAMASAKI, Masahiro
TSUBOI, Yoshihiro
TANIYAMA, Yusuke
UCHIDA, Naohiro
SATO, Reeko
NAKAMURA, Kensuke
OHTA, Hiroshi
TAKIGUCHI, Mitsuyoshi
Molecular cloning, phylogenetic analysis and heat shock response of Babesia gibsoni heat shock protein 90
title Molecular cloning, phylogenetic analysis and heat shock response of Babesia gibsoni heat shock protein 90
title_full Molecular cloning, phylogenetic analysis and heat shock response of Babesia gibsoni heat shock protein 90
title_fullStr Molecular cloning, phylogenetic analysis and heat shock response of Babesia gibsoni heat shock protein 90
title_full_unstemmed Molecular cloning, phylogenetic analysis and heat shock response of Babesia gibsoni heat shock protein 90
title_short Molecular cloning, phylogenetic analysis and heat shock response of Babesia gibsoni heat shock protein 90
title_sort molecular cloning, phylogenetic analysis and heat shock response of babesia gibsoni heat shock protein 90
topic Parasitology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5053942/
https://www.ncbi.nlm.nih.gov/pubmed/27149891
http://dx.doi.org/10.1292/jvms.16-0027
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