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Changes of Nuclear Matrix Proteins Following the Differentiation of Human Osteosarcoma MG-63 Cells
Human osteosarcoma MG-63 cells were induced into differentiation by 5 mmol/L hexamethylene bisacetamide (HMBA). Their nuclear matrix proteins (NMPs) were selectively extracted and subjected to two-dimensional gel electrophoresis analysis. The results of protein patterns were analyzed by Melanie soft...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5053975/ https://www.ncbi.nlm.nih.gov/pubmed/16689697 http://dx.doi.org/10.1016/S1672-0229(06)60011-9 |
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author | Zhao, Chun-Hong Li, Qi-Fu Zhao, Yan Niu, Jing-Wen Li, Zhi-Xing Chen, Jin-An |
author_facet | Zhao, Chun-Hong Li, Qi-Fu Zhao, Yan Niu, Jing-Wen Li, Zhi-Xing Chen, Jin-An |
author_sort | Zhao, Chun-Hong |
collection | PubMed |
description | Human osteosarcoma MG-63 cells were induced into differentiation by 5 mmol/L hexamethylene bisacetamide (HMBA). Their nuclear matrix proteins (NMPs) were selectively extracted and subjected to two-dimensional gel electrophoresis analysis. The results of protein patterns were analyzed by Melanie software. The spots of differentially expressed NMPs were excised and subjected to in situ digestion with trypsin. The maps of peptide mass fingerprinting were obtained by MALDI-TOF-MS analysis, and were submitted for NCBI database searches by Mascot tool. There were twelve spots changed remarkably during the differentiation induced by HMBA, nine of which were identified. The roles of the regulated proteins during the MG-63 differentiation were analyzed. This study suggests that the induced differentiation of cancer cells is accompanied by the changes of NMPs, and confirms the presence of some specific NMPs related to the cancer cell proliferation and differentiation. The changed NMPs are potential markers for cancer diagnosis or targets for cancer therapy. |
format | Online Article Text |
id | pubmed-5053975 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-50539752016-10-14 Changes of Nuclear Matrix Proteins Following the Differentiation of Human Osteosarcoma MG-63 Cells Zhao, Chun-Hong Li, Qi-Fu Zhao, Yan Niu, Jing-Wen Li, Zhi-Xing Chen, Jin-An Genomics Proteomics Bioinformatics Article Human osteosarcoma MG-63 cells were induced into differentiation by 5 mmol/L hexamethylene bisacetamide (HMBA). Their nuclear matrix proteins (NMPs) were selectively extracted and subjected to two-dimensional gel electrophoresis analysis. The results of protein patterns were analyzed by Melanie software. The spots of differentially expressed NMPs were excised and subjected to in situ digestion with trypsin. The maps of peptide mass fingerprinting were obtained by MALDI-TOF-MS analysis, and were submitted for NCBI database searches by Mascot tool. There were twelve spots changed remarkably during the differentiation induced by HMBA, nine of which were identified. The roles of the regulated proteins during the MG-63 differentiation were analyzed. This study suggests that the induced differentiation of cancer cells is accompanied by the changes of NMPs, and confirms the presence of some specific NMPs related to the cancer cell proliferation and differentiation. The changed NMPs are potential markers for cancer diagnosis or targets for cancer therapy. Elsevier 2006 2006-04-18 /pmc/articles/PMC5053975/ /pubmed/16689697 http://dx.doi.org/10.1016/S1672-0229(06)60011-9 Text en © 2006 Beijing Institute of Genomics http://creativecommons.org/licenses/by-nc-sa/3.0/ This is an open access article under the CC BY-NC-SA license (http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Article Zhao, Chun-Hong Li, Qi-Fu Zhao, Yan Niu, Jing-Wen Li, Zhi-Xing Chen, Jin-An Changes of Nuclear Matrix Proteins Following the Differentiation of Human Osteosarcoma MG-63 Cells |
title | Changes of Nuclear Matrix Proteins Following the Differentiation of Human Osteosarcoma MG-63 Cells |
title_full | Changes of Nuclear Matrix Proteins Following the Differentiation of Human Osteosarcoma MG-63 Cells |
title_fullStr | Changes of Nuclear Matrix Proteins Following the Differentiation of Human Osteosarcoma MG-63 Cells |
title_full_unstemmed | Changes of Nuclear Matrix Proteins Following the Differentiation of Human Osteosarcoma MG-63 Cells |
title_short | Changes of Nuclear Matrix Proteins Following the Differentiation of Human Osteosarcoma MG-63 Cells |
title_sort | changes of nuclear matrix proteins following the differentiation of human osteosarcoma mg-63 cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5053975/ https://www.ncbi.nlm.nih.gov/pubmed/16689697 http://dx.doi.org/10.1016/S1672-0229(06)60011-9 |
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