A Multifunctional Lentiviral-Based Gene Knockdown with Concurrent Rescue that Controls for Off-Target Effects of RNAi

The efficient, stable delivery of siRNA into cells, and the appropriate controls for non-specific off-target effects of siRNA are major limitations to functional studies using siRNA technology. To overcome these drawbacks, we have developed a single lentiviral vector that can concurrently deplete en...

Descripción completa

Detalles Bibliográficos
Autores principales: Feng, Yunfeng, Nie, Linghu, Thakur, Meghna Das, Su, Qin, Chi, Zhenfen, Zhao, Yongliang, Longmore, Gregory D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5054148/
https://www.ncbi.nlm.nih.gov/pubmed/21382592
http://dx.doi.org/10.1016/S1672-0229(10)60025-3

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5054148/
https://www.ncbi.nlm.nih.gov/pubmed/21382592
http://dx.doi.org/10.1016/S1672-0229(10)60025-3

Ejemplares similares