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Reconstruction of Pathways Associated with Amino Acid Metabolism in Human Mitochondria

We have used a bioinformatics approach for the identification and reconstruction of metabolic pathways associated with amino acid metabolism in human mitochondria. Human mitochondrial proteins determined by experimental and computational methods have been superposed on the reference pathways from th...

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Detalles Bibliográficos
Autores principales: Guda, Purnima, Guda, Chittibabu, Subramaniam, Shankar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5054205/
https://www.ncbi.nlm.nih.gov/pubmed/18267298
http://dx.doi.org/10.1016/S1672-0229(08)60004-2
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author Guda, Purnima
Guda, Chittibabu
Subramaniam, Shankar
author_facet Guda, Purnima
Guda, Chittibabu
Subramaniam, Shankar
author_sort Guda, Purnima
collection PubMed
description We have used a bioinformatics approach for the identification and reconstruction of metabolic pathways associated with amino acid metabolism in human mitochondria. Human mitochondrial proteins determined by experimental and computational methods have been superposed on the reference pathways from the KEGG database to identify mitochondrial pathways. Enzymes at the entry and exit points for each reconstructed pathway were identified, and mitochondrial solute carrier proteins were determined where applicable. Intermediate enzymes in the mitochondrial pathways were identified based on the annotations available from public databases, evidence in current literature, or our MITOPRED program, which predicts the mitochondrial localization of proteins. Through integration of the data derived from experimental, bibliographical, and computational sources, we reconstructed the amino acid metabolic pathways in human mitochondria, which could help better understand the mitochondrial metabolism and its role in human health.
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spelling pubmed-50542052016-10-14 Reconstruction of Pathways Associated with Amino Acid Metabolism in Human Mitochondria Guda, Purnima Guda, Chittibabu Subramaniam, Shankar Genomics Proteomics Bioinformatics Article We have used a bioinformatics approach for the identification and reconstruction of metabolic pathways associated with amino acid metabolism in human mitochondria. Human mitochondrial proteins determined by experimental and computational methods have been superposed on the reference pathways from the KEGG database to identify mitochondrial pathways. Enzymes at the entry and exit points for each reconstructed pathway were identified, and mitochondrial solute carrier proteins were determined where applicable. Intermediate enzymes in the mitochondrial pathways were identified based on the annotations available from public databases, evidence in current literature, or our MITOPRED program, which predicts the mitochondrial localization of proteins. Through integration of the data derived from experimental, bibliographical, and computational sources, we reconstructed the amino acid metabolic pathways in human mitochondria, which could help better understand the mitochondrial metabolism and its role in human health. Elsevier 2007 2008-02-08 /pmc/articles/PMC5054205/ /pubmed/18267298 http://dx.doi.org/10.1016/S1672-0229(08)60004-2 Text en © 2007 Beijing Institute of Genomics http://creativecommons.org/licenses/by-nc-sa/3.0/ This is an open access article under the CC BY-NC-SA license (http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Article
Guda, Purnima
Guda, Chittibabu
Subramaniam, Shankar
Reconstruction of Pathways Associated with Amino Acid Metabolism in Human Mitochondria
title Reconstruction of Pathways Associated with Amino Acid Metabolism in Human Mitochondria
title_full Reconstruction of Pathways Associated with Amino Acid Metabolism in Human Mitochondria
title_fullStr Reconstruction of Pathways Associated with Amino Acid Metabolism in Human Mitochondria
title_full_unstemmed Reconstruction of Pathways Associated with Amino Acid Metabolism in Human Mitochondria
title_short Reconstruction of Pathways Associated with Amino Acid Metabolism in Human Mitochondria
title_sort reconstruction of pathways associated with amino acid metabolism in human mitochondria
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5054205/
https://www.ncbi.nlm.nih.gov/pubmed/18267298
http://dx.doi.org/10.1016/S1672-0229(08)60004-2
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