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16S rDNA-Based Phylogeny of Non-Symbiotic Bacteria of Entomopathogenic Nematodes from Infected Insect Cadavers

Using 16S rDNA gene sequencing technique, three different species of non-symbiotic bacteria of entomopathogenic nematodes (EPNs) (Steinernema sp. and Heterorhabditis sp.) were isolated and identified from infected insect cadavers (Galleria mellonella larvae) after 48-hour post infections. Sequence s...

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Autores principales: Razia, M., Karthik Raja, R., Padmanaban, K., Chellapandi, P., Sivaramakrishnan, S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5054454/
https://www.ncbi.nlm.nih.gov/pubmed/21802047
http://dx.doi.org/10.1016/S1672-0229(11)60013-2
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author Razia, M.
Karthik Raja, R.
Padmanaban, K.
Chellapandi, P.
Sivaramakrishnan, S.
author_facet Razia, M.
Karthik Raja, R.
Padmanaban, K.
Chellapandi, P.
Sivaramakrishnan, S.
author_sort Razia, M.
collection PubMed
description Using 16S rDNA gene sequencing technique, three different species of non-symbiotic bacteria of entomopathogenic nematodes (EPNs) (Steinernema sp. and Heterorhabditis sp.) were isolated and identified from infected insect cadavers (Galleria mellonella larvae) after 48-hour post infections. Sequence similarity analysis revealed that the strains SRK3, SRK4 and SRK5 belong to Ochrobactrum cytisi, Schineria larvae and Ochrobactrum anthropi, respectively. The isolates O. anthropi and S. larvae were found to be associated with Heterorhabditis indica strains BDU-17 and Yer-136, respectively, whereas O. cytisi was associated with Steinernema siamkayai strain BDU-87. Phenotypically, temporal EPN bacteria were fairly related to symbiotic EPN bacteria (Photorhabdus and Xenorhabdus genera). The strains SRK3 and SRK5 were phylogeographically similar to several non-symbionts and contaminated EPN bacteria isolated in Germany (LMG3311T) and China (X-14), while the strain SRK4 was identical to the isolates of S. larvae (L1/57, L1/58, L1/68 and L2/11) from Wohlfahrtia magnifica in Hungary. The result was further confirmed by RNA secondary structure and minimum energy calculations of aligned sequences. This study suggested that the non-symbionts of these nematodes are phylogeographically diverged in some extent due to phase variation. Therefore, these strains are not host-dependent, but environment-specific isolates.
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spelling pubmed-50544542016-10-14 16S rDNA-Based Phylogeny of Non-Symbiotic Bacteria of Entomopathogenic Nematodes from Infected Insect Cadavers Razia, M. Karthik Raja, R. Padmanaban, K. Chellapandi, P. Sivaramakrishnan, S. Genomics Proteomics Bioinformatics Article Using 16S rDNA gene sequencing technique, three different species of non-symbiotic bacteria of entomopathogenic nematodes (EPNs) (Steinernema sp. and Heterorhabditis sp.) were isolated and identified from infected insect cadavers (Galleria mellonella larvae) after 48-hour post infections. Sequence similarity analysis revealed that the strains SRK3, SRK4 and SRK5 belong to Ochrobactrum cytisi, Schineria larvae and Ochrobactrum anthropi, respectively. The isolates O. anthropi and S. larvae were found to be associated with Heterorhabditis indica strains BDU-17 and Yer-136, respectively, whereas O. cytisi was associated with Steinernema siamkayai strain BDU-87. Phenotypically, temporal EPN bacteria were fairly related to symbiotic EPN bacteria (Photorhabdus and Xenorhabdus genera). The strains SRK3 and SRK5 were phylogeographically similar to several non-symbionts and contaminated EPN bacteria isolated in Germany (LMG3311T) and China (X-14), while the strain SRK4 was identical to the isolates of S. larvae (L1/57, L1/58, L1/68 and L2/11) from Wohlfahrtia magnifica in Hungary. The result was further confirmed by RNA secondary structure and minimum energy calculations of aligned sequences. This study suggested that the non-symbionts of these nematodes are phylogeographically diverged in some extent due to phase variation. Therefore, these strains are not host-dependent, but environment-specific isolates. Elsevier 2011-06 2011-07-27 /pmc/articles/PMC5054454/ /pubmed/21802047 http://dx.doi.org/10.1016/S1672-0229(11)60013-2 Text en © 2011 Beijing Institute of Genomics http://creativecommons.org/licenses/by-nc-sa/3.0/ This is an open access article under the CC BY-NC-SA license (http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Article
Razia, M.
Karthik Raja, R.
Padmanaban, K.
Chellapandi, P.
Sivaramakrishnan, S.
16S rDNA-Based Phylogeny of Non-Symbiotic Bacteria of Entomopathogenic Nematodes from Infected Insect Cadavers
title 16S rDNA-Based Phylogeny of Non-Symbiotic Bacteria of Entomopathogenic Nematodes from Infected Insect Cadavers
title_full 16S rDNA-Based Phylogeny of Non-Symbiotic Bacteria of Entomopathogenic Nematodes from Infected Insect Cadavers
title_fullStr 16S rDNA-Based Phylogeny of Non-Symbiotic Bacteria of Entomopathogenic Nematodes from Infected Insect Cadavers
title_full_unstemmed 16S rDNA-Based Phylogeny of Non-Symbiotic Bacteria of Entomopathogenic Nematodes from Infected Insect Cadavers
title_short 16S rDNA-Based Phylogeny of Non-Symbiotic Bacteria of Entomopathogenic Nematodes from Infected Insect Cadavers
title_sort 16s rdna-based phylogeny of non-symbiotic bacteria of entomopathogenic nematodes from infected insect cadavers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5054454/
https://www.ncbi.nlm.nih.gov/pubmed/21802047
http://dx.doi.org/10.1016/S1672-0229(11)60013-2
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