Cargando…

Absence of convincing evidence of Coxiella burnetii infection in Chile: a cross-sectional serosurvey among healthy adults in four different regions

BACKGROUND: Coxiella burnetii is an important zoonotic pathogen of global distribution. Still, in most parts of South America including Chile, systematic epidemiological data are lacking. The presented study aims to determine the seroprevalence of Coxiella burnetii antibodies in healthy adults of fo...

Descripción completa

Detalles Bibliográficos
Autores principales: Weitzel, Thomas, López, Javier, Acosta-Jamett, Gerardo, Edouard, Sophie, Parola, Philippe, Abarca, Katia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5054538/
https://www.ncbi.nlm.nih.gov/pubmed/27716089
http://dx.doi.org/10.1186/s12879-016-1880-9
_version_ 1782458620436283392
author Weitzel, Thomas
López, Javier
Acosta-Jamett, Gerardo
Edouard, Sophie
Parola, Philippe
Abarca, Katia
author_facet Weitzel, Thomas
López, Javier
Acosta-Jamett, Gerardo
Edouard, Sophie
Parola, Philippe
Abarca, Katia
author_sort Weitzel, Thomas
collection PubMed
description BACKGROUND: Coxiella burnetii is an important zoonotic pathogen of global distribution. Still, in most parts of South America including Chile, systematic epidemiological data are lacking. The presented study aims to determine the seroprevalence of Coxiella burnetii antibodies in healthy adults of four different regions in Chile. METHODS: A cross-sectional study was performed, which included healthy adults living in rural and urban areas of four cities located in different regions in northern, central, and southern Chile. In urban sectors, households were chosen by double stratified random sampling, while in rural areas convenience sampling was performed. Serum specimens were taken and screened for the presence of IgG antibodies against C. burnetii phase II antigen using a commercial ELISA kit. Positive and indeterminate results were confirmed by a reference laboratory using indirect immunofluorescence assay (IFA). RESULTS: A total of 1112 individuals were included. Of those, 8 were positive by ELISA, but only one sample was confirmed using IFA. Statistical analysis for population freedom from disease revealed a high probability that C. burnetii was absent in our study population. CONCLUSION: Our work provides the first epidemiological data on human Q fever in Chile indicating either a very low endemicity or the absence of this pathogen in the studied areas. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12879-016-1880-9) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-5054538
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-50545382016-10-19 Absence of convincing evidence of Coxiella burnetii infection in Chile: a cross-sectional serosurvey among healthy adults in four different regions Weitzel, Thomas López, Javier Acosta-Jamett, Gerardo Edouard, Sophie Parola, Philippe Abarca, Katia BMC Infect Dis Research Article BACKGROUND: Coxiella burnetii is an important zoonotic pathogen of global distribution. Still, in most parts of South America including Chile, systematic epidemiological data are lacking. The presented study aims to determine the seroprevalence of Coxiella burnetii antibodies in healthy adults of four different regions in Chile. METHODS: A cross-sectional study was performed, which included healthy adults living in rural and urban areas of four cities located in different regions in northern, central, and southern Chile. In urban sectors, households were chosen by double stratified random sampling, while in rural areas convenience sampling was performed. Serum specimens were taken and screened for the presence of IgG antibodies against C. burnetii phase II antigen using a commercial ELISA kit. Positive and indeterminate results were confirmed by a reference laboratory using indirect immunofluorescence assay (IFA). RESULTS: A total of 1112 individuals were included. Of those, 8 were positive by ELISA, but only one sample was confirmed using IFA. Statistical analysis for population freedom from disease revealed a high probability that C. burnetii was absent in our study population. CONCLUSION: Our work provides the first epidemiological data on human Q fever in Chile indicating either a very low endemicity or the absence of this pathogen in the studied areas. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12879-016-1880-9) contains supplementary material, which is available to authorized users. BioMed Central 2016-10-06 /pmc/articles/PMC5054538/ /pubmed/27716089 http://dx.doi.org/10.1186/s12879-016-1880-9 Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Weitzel, Thomas
López, Javier
Acosta-Jamett, Gerardo
Edouard, Sophie
Parola, Philippe
Abarca, Katia
Absence of convincing evidence of Coxiella burnetii infection in Chile: a cross-sectional serosurvey among healthy adults in four different regions
title Absence of convincing evidence of Coxiella burnetii infection in Chile: a cross-sectional serosurvey among healthy adults in four different regions
title_full Absence of convincing evidence of Coxiella burnetii infection in Chile: a cross-sectional serosurvey among healthy adults in four different regions
title_fullStr Absence of convincing evidence of Coxiella burnetii infection in Chile: a cross-sectional serosurvey among healthy adults in four different regions
title_full_unstemmed Absence of convincing evidence of Coxiella burnetii infection in Chile: a cross-sectional serosurvey among healthy adults in four different regions
title_short Absence of convincing evidence of Coxiella burnetii infection in Chile: a cross-sectional serosurvey among healthy adults in four different regions
title_sort absence of convincing evidence of coxiella burnetii infection in chile: a cross-sectional serosurvey among healthy adults in four different regions
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5054538/
https://www.ncbi.nlm.nih.gov/pubmed/27716089
http://dx.doi.org/10.1186/s12879-016-1880-9
work_keys_str_mv AT weitzelthomas absenceofconvincingevidenceofcoxiellaburnetiiinfectioninchileacrosssectionalserosurveyamonghealthyadultsinfourdifferentregions
AT lopezjavier absenceofconvincingevidenceofcoxiellaburnetiiinfectioninchileacrosssectionalserosurveyamonghealthyadultsinfourdifferentregions
AT acostajamettgerardo absenceofconvincingevidenceofcoxiellaburnetiiinfectioninchileacrosssectionalserosurveyamonghealthyadultsinfourdifferentregions
AT edouardsophie absenceofconvincingevidenceofcoxiellaburnetiiinfectioninchileacrosssectionalserosurveyamonghealthyadultsinfourdifferentregions
AT parolaphilippe absenceofconvincingevidenceofcoxiellaburnetiiinfectioninchileacrosssectionalserosurveyamonghealthyadultsinfourdifferentregions
AT abarcakatia absenceofconvincingevidenceofcoxiellaburnetiiinfectioninchileacrosssectionalserosurveyamonghealthyadultsinfourdifferentregions