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Molecular evolution of Odorant-binding proteins gene family in two closely related Anastrepha fruit flies

BACKGROUND: Odorant-binding proteins (OBPs) are of great importance for survival and reproduction since they participate in initial steps of the olfactory signal transduction cascade, solubilizing and transporting chemical signals to the olfactory receptors. A comparative analysis of OBPs between cl...

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Autores principales: Campanini, Emeline Boni, de Brito, Reinaldo Alves
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5054612/
https://www.ncbi.nlm.nih.gov/pubmed/27716035
http://dx.doi.org/10.1186/s12862-016-0775-0
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author Campanini, Emeline Boni
de Brito, Reinaldo Alves
author_facet Campanini, Emeline Boni
de Brito, Reinaldo Alves
author_sort Campanini, Emeline Boni
collection PubMed
description BACKGROUND: Odorant-binding proteins (OBPs) are of great importance for survival and reproduction since they participate in initial steps of the olfactory signal transduction cascade, solubilizing and transporting chemical signals to the olfactory receptors. A comparative analysis of OBPs between closely related species may help explain how these genes evolve and are maintained under natural selection and how differences in these proteins can affect olfactory responses. We studied OBP genes in the closely related species Anastrepha fraterculus and A. obliqua, which have different host preferences, using data from RNA-seq cDNA libraries of head and reproductive tissues from male and female adults, aiming to understand the speciation process occurred between them. RESULTS: We identified 23 different OBP sequences from Anastrepha fraterculus and 24 from A. obliqua, which correspond to 20 Drosophila melanogaster OBP genes. Phylogenetic analysis separated Anastrepha OBPs sequences in four branches that represent four subfamilies: classic, minus-C, plus-C and dimer. Both species showed five plus-C members, which is the biggest number found in tephritids until now. We found evidence of positive selection in four genes and at least one duplication event that preceded the speciation of these two species. Inferences on tertiary structures of putative proteins from these genes revealed that at least one positively selected change involves the binding cavity (the odorant binding region) in the plus-C OBP50a. CONCLUSIONS: A. fraterculus and A. obliqua have a bigger OBP repertoire than the other tephritids studied, though the total number of Anastrepha OBPs may be larger, since we studied only a limited number of tissues. The contrast of these closely related species reveals that there are several amino acid changes between the homologous genes, which might be related to their host preferences. The plus-C OBP that has one amino acid under positive selection located in the binding cavity may be under a selection pressure to recognize and bind a new odorant. The other positively selected sites found may be involved in important structural and functional changes, especially ones in which site-specific changes would radically change amino acid properties. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12862-016-0775-0) contains supplementary material, which is available to authorized users.
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spelling pubmed-50546122016-10-19 Molecular evolution of Odorant-binding proteins gene family in two closely related Anastrepha fruit flies Campanini, Emeline Boni de Brito, Reinaldo Alves BMC Evol Biol Research Article BACKGROUND: Odorant-binding proteins (OBPs) are of great importance for survival and reproduction since they participate in initial steps of the olfactory signal transduction cascade, solubilizing and transporting chemical signals to the olfactory receptors. A comparative analysis of OBPs between closely related species may help explain how these genes evolve and are maintained under natural selection and how differences in these proteins can affect olfactory responses. We studied OBP genes in the closely related species Anastrepha fraterculus and A. obliqua, which have different host preferences, using data from RNA-seq cDNA libraries of head and reproductive tissues from male and female adults, aiming to understand the speciation process occurred between them. RESULTS: We identified 23 different OBP sequences from Anastrepha fraterculus and 24 from A. obliqua, which correspond to 20 Drosophila melanogaster OBP genes. Phylogenetic analysis separated Anastrepha OBPs sequences in four branches that represent four subfamilies: classic, minus-C, plus-C and dimer. Both species showed five plus-C members, which is the biggest number found in tephritids until now. We found evidence of positive selection in four genes and at least one duplication event that preceded the speciation of these two species. Inferences on tertiary structures of putative proteins from these genes revealed that at least one positively selected change involves the binding cavity (the odorant binding region) in the plus-C OBP50a. CONCLUSIONS: A. fraterculus and A. obliqua have a bigger OBP repertoire than the other tephritids studied, though the total number of Anastrepha OBPs may be larger, since we studied only a limited number of tissues. The contrast of these closely related species reveals that there are several amino acid changes between the homologous genes, which might be related to their host preferences. The plus-C OBP that has one amino acid under positive selection located in the binding cavity may be under a selection pressure to recognize and bind a new odorant. The other positively selected sites found may be involved in important structural and functional changes, especially ones in which site-specific changes would radically change amino acid properties. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12862-016-0775-0) contains supplementary material, which is available to authorized users. BioMed Central 2016-10-07 /pmc/articles/PMC5054612/ /pubmed/27716035 http://dx.doi.org/10.1186/s12862-016-0775-0 Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Campanini, Emeline Boni
de Brito, Reinaldo Alves
Molecular evolution of Odorant-binding proteins gene family in two closely related Anastrepha fruit flies
title Molecular evolution of Odorant-binding proteins gene family in two closely related Anastrepha fruit flies
title_full Molecular evolution of Odorant-binding proteins gene family in two closely related Anastrepha fruit flies
title_fullStr Molecular evolution of Odorant-binding proteins gene family in two closely related Anastrepha fruit flies
title_full_unstemmed Molecular evolution of Odorant-binding proteins gene family in two closely related Anastrepha fruit flies
title_short Molecular evolution of Odorant-binding proteins gene family in two closely related Anastrepha fruit flies
title_sort molecular evolution of odorant-binding proteins gene family in two closely related anastrepha fruit flies
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5054612/
https://www.ncbi.nlm.nih.gov/pubmed/27716035
http://dx.doi.org/10.1186/s12862-016-0775-0
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