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Novel Adaptors of Amyloid Precursor Protein Intracellular Domain and Their Functional Implications

Amyloid precursor protein intracellular domain (AICD) is one of the potential candidates in deciphering the complexity of Alzheimer’s disease. It plays important roles in determining cell fate and neurodegeneration through its interactions with several adaptors. The presence or absence of phosphoryl...

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Detalles Bibliográficos
Autores principales: Chakrabarti, Arunabha, Mukhopadhyay, Debashis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5054717/
https://www.ncbi.nlm.nih.gov/pubmed/23084776
http://dx.doi.org/10.1016/j.gpb.2012.07.002
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author Chakrabarti, Arunabha
Mukhopadhyay, Debashis
author_facet Chakrabarti, Arunabha
Mukhopadhyay, Debashis
author_sort Chakrabarti, Arunabha
collection PubMed
description Amyloid precursor protein intracellular domain (AICD) is one of the potential candidates in deciphering the complexity of Alzheimer’s disease. It plays important roles in determining cell fate and neurodegeneration through its interactions with several adaptors. The presence or absence of phosphorylation at specific sites determines the choice of partners. In this study, we identified 20 novel AICD-interacting proteins by in vitro pull down experiments followed by 2D gel electrophoresis and MALDI-MS analysis. The identified proteins can be grouped into different functional classes including molecular chaperones, structural proteins, signaling and transport molecules, adaptors, motor proteins and apoptosis determinants. Interactions of nine proteins were further validated either by colocalization using confocal imaging or by co-immunoprecipitation followed by immunoblotting. The cellular functions of most of the proteins can be correlated with AD. Hence, illustration of their interactions with AICD may shed some light on the disease pathophysiology.
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spelling pubmed-50547172016-10-14 Novel Adaptors of Amyloid Precursor Protein Intracellular Domain and Their Functional Implications Chakrabarti, Arunabha Mukhopadhyay, Debashis Genomics Proteomics Bioinformatics Original Research Amyloid precursor protein intracellular domain (AICD) is one of the potential candidates in deciphering the complexity of Alzheimer’s disease. It plays important roles in determining cell fate and neurodegeneration through its interactions with several adaptors. The presence or absence of phosphorylation at specific sites determines the choice of partners. In this study, we identified 20 novel AICD-interacting proteins by in vitro pull down experiments followed by 2D gel electrophoresis and MALDI-MS analysis. The identified proteins can be grouped into different functional classes including molecular chaperones, structural proteins, signaling and transport molecules, adaptors, motor proteins and apoptosis determinants. Interactions of nine proteins were further validated either by colocalization using confocal imaging or by co-immunoprecipitation followed by immunoblotting. The cellular functions of most of the proteins can be correlated with AD. Hence, illustration of their interactions with AICD may shed some light on the disease pathophysiology. Elsevier 2012-08 2012-07-25 /pmc/articles/PMC5054717/ /pubmed/23084776 http://dx.doi.org/10.1016/j.gpb.2012.07.002 Text en © 2012 Beijing Institute of Genomics, Chinese Academy of Sciences and Genetics Society of China. Published by Elsevier Ltd and Science Press. All rights reserved. http://creativecommons.org/licenses/by-nc-sa/3.0/ This is an open access article under the CC BY-NC-SA license (http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Original Research
Chakrabarti, Arunabha
Mukhopadhyay, Debashis
Novel Adaptors of Amyloid Precursor Protein Intracellular Domain and Their Functional Implications
title Novel Adaptors of Amyloid Precursor Protein Intracellular Domain and Their Functional Implications
title_full Novel Adaptors of Amyloid Precursor Protein Intracellular Domain and Their Functional Implications
title_fullStr Novel Adaptors of Amyloid Precursor Protein Intracellular Domain and Their Functional Implications
title_full_unstemmed Novel Adaptors of Amyloid Precursor Protein Intracellular Domain and Their Functional Implications
title_short Novel Adaptors of Amyloid Precursor Protein Intracellular Domain and Their Functional Implications
title_sort novel adaptors of amyloid precursor protein intracellular domain and their functional implications
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5054717/
https://www.ncbi.nlm.nih.gov/pubmed/23084776
http://dx.doi.org/10.1016/j.gpb.2012.07.002
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