The long noncoding RNA CASC9 regulates migration and invasion in esophageal cancer

The objective of the study was to investigate the expression and functions of CASC9 in esophageal squamous cell carcinoma (ESCC). Long noncoding RNAs (lncRNAs) upregulated in ESCC tissues were detected by RNA sequencing. Expression of CASC9 was determined from clinical samples and cell lines by qRT‐...

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Autores principales: Pan, Zhiwen, Mao, Weimin, Bao, Yejiang, Zhang, Min, Su, Xinhua, Xu, Xiaohong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Cancer Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5055159/
https://www.ncbi.nlm.nih.gov/pubmed/27431358
http://dx.doi.org/10.1002/cam4.770
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author Pan, Zhiwen
Mao, Weimin
Bao, Yejiang
Zhang, Min
Su, Xinhua
Xu, Xiaohong
author_facet Pan, Zhiwen
Mao, Weimin
Bao, Yejiang
Zhang, Min
Su, Xinhua
Xu, Xiaohong
author_sort Pan, Zhiwen
collection PubMed
description The objective of the study was to investigate the expression and functions of CASC9 in esophageal squamous cell carcinoma (ESCC). Long noncoding RNAs (lncRNAs) upregulated in ESCC tissues were detected by RNA sequencing. Expression of CASC9 was determined from clinical samples and cell lines by qRT‐PCR. The effects of CASC9 knockdown on migration and invasion were evaluated by wound healing assay, cell migration and invasion assays in vitro. We found that the lncRNA, CASC9, was markedly upregulated in ESCC tissues. Furthermore, knockdown of CASC9 significantly suppressed cell migration and invasion in vitro. Furthermore, enhanced CASC9 expression level was correlated with differentiation. The results indicated that CASC9 is significantly upregulated in ESCC tissues and may represent a new marker of poor prognosis and a potential therapeutic target for esophageal cancer intervention.
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spelling pubmed-50551592016-12-12 The long noncoding RNA CASC9 regulates migration and invasion in esophageal cancer Pan, Zhiwen Mao, Weimin Bao, Yejiang Zhang, Min Su, Xinhua Xu, Xiaohong Cancer Med Cancer Biology The objective of the study was to investigate the expression and functions of CASC9 in esophageal squamous cell carcinoma (ESCC). Long noncoding RNAs (lncRNAs) upregulated in ESCC tissues were detected by RNA sequencing. Expression of CASC9 was determined from clinical samples and cell lines by qRT‐PCR. The effects of CASC9 knockdown on migration and invasion were evaluated by wound healing assay, cell migration and invasion assays in vitro. We found that the lncRNA, CASC9, was markedly upregulated in ESCC tissues. Furthermore, knockdown of CASC9 significantly suppressed cell migration and invasion in vitro. Furthermore, enhanced CASC9 expression level was correlated with differentiation. The results indicated that CASC9 is significantly upregulated in ESCC tissues and may represent a new marker of poor prognosis and a potential therapeutic target for esophageal cancer intervention. John Wiley and Sons Inc. 2016-07-19 /pmc/articles/PMC5055159/ /pubmed/27431358 http://dx.doi.org/10.1002/cam4.770 Text en © 2016 The Authors. Cancer Medicine published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Cancer Biology
Pan, Zhiwen
Mao, Weimin
Bao, Yejiang
Zhang, Min
Su, Xinhua
Xu, Xiaohong
The long noncoding RNA CASC9 regulates migration and invasion in esophageal cancer
title The long noncoding RNA CASC9 regulates migration and invasion in esophageal cancer
title_full The long noncoding RNA CASC9 regulates migration and invasion in esophageal cancer
title_fullStr The long noncoding RNA CASC9 regulates migration and invasion in esophageal cancer
title_full_unstemmed The long noncoding RNA CASC9 regulates migration and invasion in esophageal cancer
title_short The long noncoding RNA CASC9 regulates migration and invasion in esophageal cancer
title_sort long noncoding rna casc9 regulates migration and invasion in esophageal cancer
topic Cancer Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5055159/
https://www.ncbi.nlm.nih.gov/pubmed/27431358
http://dx.doi.org/10.1002/cam4.770
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