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Functional Properties of Mouse Chitotriosidase Expressed in the Periplasmic Space of Escherichia coli

Chitotriosidase (Chit1) is an enzyme associated with various diseases, including Gaucher disease, chronic obstructive pulmonary disease, Alzheimer disease and cystic fibrosis. In this study, we first expressed mouse mature Chit1 fused with V5 and (His)(6) tags at the C-terminus (Chit1-V5-His) in the...

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Detalles Bibliográficos
Autores principales: Kimura, Masahiro, Wakita, Satoshi, Ishikawa, Kotarou, Sekine, Kazutaka, Yoshikawa, Satoshi, Sato, Akira, Okawa, Kazuaki, Kashimura, Akinori, Sakaguchi, Masayoshi, Sugahara, Yasusato, Yamanaka, Daisuke, Ohno, Naohito, Bauer, Peter O, Oyama, Fumitaka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5055312/
https://www.ncbi.nlm.nih.gov/pubmed/27716783
http://dx.doi.org/10.1371/journal.pone.0164367