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Functional Properties of Mouse Chitotriosidase Expressed in the Periplasmic Space of Escherichia coli
Chitotriosidase (Chit1) is an enzyme associated with various diseases, including Gaucher disease, chronic obstructive pulmonary disease, Alzheimer disease and cystic fibrosis. In this study, we first expressed mouse mature Chit1 fused with V5 and (His)(6) tags at the C-terminus (Chit1-V5-His) in the...
Autores principales: | Kimura, Masahiro, Wakita, Satoshi, Ishikawa, Kotarou, Sekine, Kazutaka, Yoshikawa, Satoshi, Sato, Akira, Okawa, Kazuaki, Kashimura, Akinori, Sakaguchi, Masayoshi, Sugahara, Yasusato, Yamanaka, Daisuke, Ohno, Naohito, Bauer, Peter O, Oyama, Fumitaka |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5055312/ https://www.ncbi.nlm.nih.gov/pubmed/27716783 http://dx.doi.org/10.1371/journal.pone.0164367 |
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