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Cadherin-2 Is Required Cell Autonomously for Collective Migration of Facial Branchiomotor Neurons

Collective migration depends on cell-cell interactions between neighbors that contribute to their overall directionality, yet the mechanisms that control the coordinated migration of neurons remains to be elucidated. During hindbrain development, facial branchiomotor neurons (FBMNs) undergo a stereo...

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Autores principales: Rebman, Jane K., Kirchoff, Kathryn E., Walsh, Gregory S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5055392/
https://www.ncbi.nlm.nih.gov/pubmed/27716840
http://dx.doi.org/10.1371/journal.pone.0164433
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author Rebman, Jane K.
Kirchoff, Kathryn E.
Walsh, Gregory S.
author_facet Rebman, Jane K.
Kirchoff, Kathryn E.
Walsh, Gregory S.
author_sort Rebman, Jane K.
collection PubMed
description Collective migration depends on cell-cell interactions between neighbors that contribute to their overall directionality, yet the mechanisms that control the coordinated migration of neurons remains to be elucidated. During hindbrain development, facial branchiomotor neurons (FBMNs) undergo a stereotypic tangential caudal migration from their place of birth in rhombomere (r)4 to their final location in r6/7. FBMNs engage in collective cell migration that depends on neuron-to-neuron interactions to facilitate caudal directionality. Here, we demonstrate that Cadherin-2-mediated neuron-to-neuron adhesion is necessary for directional and collective migration of FBMNs. We generated stable transgenic zebrafish expressing dominant-negative Cadherin-2 (Cdh2ΔEC) driven by the islet1 promoter. Cell-autonomous inactivation of Cadherin-2 function led to non-directional migration of FBMNs and a defect in caudal tangential migration. Additionally, mosaic analysis revealed that Cdh2ΔEC-expressing FBMNs are not influenced to migrate caudally by neighboring wild-type FBMNs due to a defect in collective cell migration. Taken together, our data suggest that Cadherin-2 plays an essential cell-autonomous role in mediating the collective migration of FBMNs.
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spelling pubmed-50553922016-10-27 Cadherin-2 Is Required Cell Autonomously for Collective Migration of Facial Branchiomotor Neurons Rebman, Jane K. Kirchoff, Kathryn E. Walsh, Gregory S. PLoS One Research Article Collective migration depends on cell-cell interactions between neighbors that contribute to their overall directionality, yet the mechanisms that control the coordinated migration of neurons remains to be elucidated. During hindbrain development, facial branchiomotor neurons (FBMNs) undergo a stereotypic tangential caudal migration from their place of birth in rhombomere (r)4 to their final location in r6/7. FBMNs engage in collective cell migration that depends on neuron-to-neuron interactions to facilitate caudal directionality. Here, we demonstrate that Cadherin-2-mediated neuron-to-neuron adhesion is necessary for directional and collective migration of FBMNs. We generated stable transgenic zebrafish expressing dominant-negative Cadherin-2 (Cdh2ΔEC) driven by the islet1 promoter. Cell-autonomous inactivation of Cadherin-2 function led to non-directional migration of FBMNs and a defect in caudal tangential migration. Additionally, mosaic analysis revealed that Cdh2ΔEC-expressing FBMNs are not influenced to migrate caudally by neighboring wild-type FBMNs due to a defect in collective cell migration. Taken together, our data suggest that Cadherin-2 plays an essential cell-autonomous role in mediating the collective migration of FBMNs. Public Library of Science 2016-10-07 /pmc/articles/PMC5055392/ /pubmed/27716840 http://dx.doi.org/10.1371/journal.pone.0164433 Text en © 2016 Rebman et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Rebman, Jane K.
Kirchoff, Kathryn E.
Walsh, Gregory S.
Cadherin-2 Is Required Cell Autonomously for Collective Migration of Facial Branchiomotor Neurons
title Cadherin-2 Is Required Cell Autonomously for Collective Migration of Facial Branchiomotor Neurons
title_full Cadherin-2 Is Required Cell Autonomously for Collective Migration of Facial Branchiomotor Neurons
title_fullStr Cadherin-2 Is Required Cell Autonomously for Collective Migration of Facial Branchiomotor Neurons
title_full_unstemmed Cadherin-2 Is Required Cell Autonomously for Collective Migration of Facial Branchiomotor Neurons
title_short Cadherin-2 Is Required Cell Autonomously for Collective Migration of Facial Branchiomotor Neurons
title_sort cadherin-2 is required cell autonomously for collective migration of facial branchiomotor neurons
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5055392/
https://www.ncbi.nlm.nih.gov/pubmed/27716840
http://dx.doi.org/10.1371/journal.pone.0164433
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