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Modern morphological engineering techniques for improving productivity of filamentous fungi in submerged cultures

Morphological engineering techniques have recently gained popularity as they are used for increasing the productivity of a variety of metabolites and enzymes in fungi growing in submerged cultures. Their action is mainly associated with the changes they evoke in fungal morphology. Traditional morpho...

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Autores principales: Antecka, Anna, Bizukojc, Marcin, Ledakowicz, Stanislaw
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5055562/
https://www.ncbi.nlm.nih.gov/pubmed/27718148
http://dx.doi.org/10.1007/s11274-016-2148-7
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author Antecka, Anna
Bizukojc, Marcin
Ledakowicz, Stanislaw
author_facet Antecka, Anna
Bizukojc, Marcin
Ledakowicz, Stanislaw
author_sort Antecka, Anna
collection PubMed
description Morphological engineering techniques have recently gained popularity as they are used for increasing the productivity of a variety of metabolites and enzymes in fungi growing in submerged cultures. Their action is mainly associated with the changes they evoke in fungal morphology. Traditional morphological engineering approaches include manipulation of spore concentration, pH-shifting and mechanical stress exerted by stirring and aeration. As the traditional methods proved to be insufficient, modern techniques such as changes of medium osmolality or addition of mineral microparticles to the media (microparticle-enhanced cultivation, MPEC) were proposed. Despite the fact that this area of knowledge is still being developed, there are a fair amount of scientific articles concerning the cultivations of filamentous fungi with the use of these techniques. It was described that in Ascomycetes fungi both MPEC or change of osmolality successfully led to the change of mycelial morphology, which appeared to be favorable for increased productivity of secondary metabolites and enzymes. There are also limited but very promising reports involving the successful application of MPEC with Basidiomycetes species. Despite the fact that the mineral microparticles behave differently for various microorganisms, being strain and particle specific, the low cost of its application is a great benefit. This paper reviews the application of the modern morphology engineering techniques. The authors critically assess the advantages, shortcomings, and future prospects of their application in the cultivation of fungi.
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spelling pubmed-50555622016-10-26 Modern morphological engineering techniques for improving productivity of filamentous fungi in submerged cultures Antecka, Anna Bizukojc, Marcin Ledakowicz, Stanislaw World J Microbiol Biotechnol Review Morphological engineering techniques have recently gained popularity as they are used for increasing the productivity of a variety of metabolites and enzymes in fungi growing in submerged cultures. Their action is mainly associated with the changes they evoke in fungal morphology. Traditional morphological engineering approaches include manipulation of spore concentration, pH-shifting and mechanical stress exerted by stirring and aeration. As the traditional methods proved to be insufficient, modern techniques such as changes of medium osmolality or addition of mineral microparticles to the media (microparticle-enhanced cultivation, MPEC) were proposed. Despite the fact that this area of knowledge is still being developed, there are a fair amount of scientific articles concerning the cultivations of filamentous fungi with the use of these techniques. It was described that in Ascomycetes fungi both MPEC or change of osmolality successfully led to the change of mycelial morphology, which appeared to be favorable for increased productivity of secondary metabolites and enzymes. There are also limited but very promising reports involving the successful application of MPEC with Basidiomycetes species. Despite the fact that the mineral microparticles behave differently for various microorganisms, being strain and particle specific, the low cost of its application is a great benefit. This paper reviews the application of the modern morphology engineering techniques. The authors critically assess the advantages, shortcomings, and future prospects of their application in the cultivation of fungi. Springer Netherlands 2016-10-07 2016 /pmc/articles/PMC5055562/ /pubmed/27718148 http://dx.doi.org/10.1007/s11274-016-2148-7 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Review
Antecka, Anna
Bizukojc, Marcin
Ledakowicz, Stanislaw
Modern morphological engineering techniques for improving productivity of filamentous fungi in submerged cultures
title Modern morphological engineering techniques for improving productivity of filamentous fungi in submerged cultures
title_full Modern morphological engineering techniques for improving productivity of filamentous fungi in submerged cultures
title_fullStr Modern morphological engineering techniques for improving productivity of filamentous fungi in submerged cultures
title_full_unstemmed Modern morphological engineering techniques for improving productivity of filamentous fungi in submerged cultures
title_short Modern morphological engineering techniques for improving productivity of filamentous fungi in submerged cultures
title_sort modern morphological engineering techniques for improving productivity of filamentous fungi in submerged cultures
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5055562/
https://www.ncbi.nlm.nih.gov/pubmed/27718148
http://dx.doi.org/10.1007/s11274-016-2148-7
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