Cargando…

Members of WRKY Group III transcription factors are important in TYLCV defense signaling pathway in tomato (Solanum lycopersicum)

BACKGROUND: Transmitted by the whitefly Bemisia tabaci, tomato yellow leaf curly virus (TYLCV) has posed serious threats to plant growth and development. Plant innate immune systems against various threats involve WRKY Group III transcription factors (TFs). This group participates as a major compone...

Descripción completa

Detalles Bibliográficos
Autores principales: Huang, Ying, Li, Meng-Yao, Wu, Peng, Xu, Zhi-Sheng, Que, Feng, Wang, Feng, Xiong, Ai-Sheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5055730/
https://www.ncbi.nlm.nih.gov/pubmed/27717312
http://dx.doi.org/10.1186/s12864-016-3123-2
_version_ 1782458805495267328
author Huang, Ying
Li, Meng-Yao
Wu, Peng
Xu, Zhi-Sheng
Que, Feng
Wang, Feng
Xiong, Ai-Sheng
author_facet Huang, Ying
Li, Meng-Yao
Wu, Peng
Xu, Zhi-Sheng
Que, Feng
Wang, Feng
Xiong, Ai-Sheng
author_sort Huang, Ying
collection PubMed
description BACKGROUND: Transmitted by the whitefly Bemisia tabaci, tomato yellow leaf curly virus (TYLCV) has posed serious threats to plant growth and development. Plant innate immune systems against various threats involve WRKY Group III transcription factors (TFs). This group participates as a major component of biological processes in plants. RESULTS: In this study, 6 WRKY Group III TFs (SolyWRKY41, SolyWRKY42, SolyWRKY53, SolyWRKY54, SolyWRKY80, and SolyWRKY81) were identified, and these TFs responded to TYLCV infection. Subcellular localization analysis indicated that SolyWRKY41 and SolyWRKY54 were nuclear proteins in vivo. Many elements, including W-box, were found in the promoter region of Group III TFs. Interaction network analysis revealed that Group III TFs could interact with other proteins, such as mitogen-activated protein kinase 5 (MAPK) and isochorismate synthase (ICS), to respond to biotic and abiotic stresses. Positive and negative expression patterns showed that WRKY Group III genes could also respond to TYLCV infection in tomato. The DNA content of TYLCV resistant lines after SolyWRKY41 and SolyWRKY54 were subjected to virus-induced gene silencing (VIGS) was lower than that of the control lines. CONCLUSIONS: In the present study, 6 WRKY Group III TFs in tomato were identified to respond to TYLCV infection. Quantitative real-time–polymerase chain reaction (RT-qPCR) and VIGS analyses demonstrated that Group III genes served as positive and negative regulators in tomato–TYLCV interaction. WRKY Group III TFs could interact with other proteins by binding to cis elements existing in the promoter regions of other genes to regulate pathogen-related gene expression. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12864-016-3123-2) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-5055730
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-50557302016-10-19 Members of WRKY Group III transcription factors are important in TYLCV defense signaling pathway in tomato (Solanum lycopersicum) Huang, Ying Li, Meng-Yao Wu, Peng Xu, Zhi-Sheng Que, Feng Wang, Feng Xiong, Ai-Sheng BMC Genomics Research Article BACKGROUND: Transmitted by the whitefly Bemisia tabaci, tomato yellow leaf curly virus (TYLCV) has posed serious threats to plant growth and development. Plant innate immune systems against various threats involve WRKY Group III transcription factors (TFs). This group participates as a major component of biological processes in plants. RESULTS: In this study, 6 WRKY Group III TFs (SolyWRKY41, SolyWRKY42, SolyWRKY53, SolyWRKY54, SolyWRKY80, and SolyWRKY81) were identified, and these TFs responded to TYLCV infection. Subcellular localization analysis indicated that SolyWRKY41 and SolyWRKY54 were nuclear proteins in vivo. Many elements, including W-box, were found in the promoter region of Group III TFs. Interaction network analysis revealed that Group III TFs could interact with other proteins, such as mitogen-activated protein kinase 5 (MAPK) and isochorismate synthase (ICS), to respond to biotic and abiotic stresses. Positive and negative expression patterns showed that WRKY Group III genes could also respond to TYLCV infection in tomato. The DNA content of TYLCV resistant lines after SolyWRKY41 and SolyWRKY54 were subjected to virus-induced gene silencing (VIGS) was lower than that of the control lines. CONCLUSIONS: In the present study, 6 WRKY Group III TFs in tomato were identified to respond to TYLCV infection. Quantitative real-time–polymerase chain reaction (RT-qPCR) and VIGS analyses demonstrated that Group III genes served as positive and negative regulators in tomato–TYLCV interaction. WRKY Group III TFs could interact with other proteins by binding to cis elements existing in the promoter regions of other genes to regulate pathogen-related gene expression. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12864-016-3123-2) contains supplementary material, which is available to authorized users. BioMed Central 2016-10-07 /pmc/articles/PMC5055730/ /pubmed/27717312 http://dx.doi.org/10.1186/s12864-016-3123-2 Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Huang, Ying
Li, Meng-Yao
Wu, Peng
Xu, Zhi-Sheng
Que, Feng
Wang, Feng
Xiong, Ai-Sheng
Members of WRKY Group III transcription factors are important in TYLCV defense signaling pathway in tomato (Solanum lycopersicum)
title Members of WRKY Group III transcription factors are important in TYLCV defense signaling pathway in tomato (Solanum lycopersicum)
title_full Members of WRKY Group III transcription factors are important in TYLCV defense signaling pathway in tomato (Solanum lycopersicum)
title_fullStr Members of WRKY Group III transcription factors are important in TYLCV defense signaling pathway in tomato (Solanum lycopersicum)
title_full_unstemmed Members of WRKY Group III transcription factors are important in TYLCV defense signaling pathway in tomato (Solanum lycopersicum)
title_short Members of WRKY Group III transcription factors are important in TYLCV defense signaling pathway in tomato (Solanum lycopersicum)
title_sort members of wrky group iii transcription factors are important in tylcv defense signaling pathway in tomato (solanum lycopersicum)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5055730/
https://www.ncbi.nlm.nih.gov/pubmed/27717312
http://dx.doi.org/10.1186/s12864-016-3123-2
work_keys_str_mv AT huangying membersofwrkygroupiiitranscriptionfactorsareimportantintylcvdefensesignalingpathwayintomatosolanumlycopersicum
AT limengyao membersofwrkygroupiiitranscriptionfactorsareimportantintylcvdefensesignalingpathwayintomatosolanumlycopersicum
AT wupeng membersofwrkygroupiiitranscriptionfactorsareimportantintylcvdefensesignalingpathwayintomatosolanumlycopersicum
AT xuzhisheng membersofwrkygroupiiitranscriptionfactorsareimportantintylcvdefensesignalingpathwayintomatosolanumlycopersicum
AT quefeng membersofwrkygroupiiitranscriptionfactorsareimportantintylcvdefensesignalingpathwayintomatosolanumlycopersicum
AT wangfeng membersofwrkygroupiiitranscriptionfactorsareimportantintylcvdefensesignalingpathwayintomatosolanumlycopersicum
AT xiongaisheng membersofwrkygroupiiitranscriptionfactorsareimportantintylcvdefensesignalingpathwayintomatosolanumlycopersicum