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DNA methylation landscape of fat deposits and fatty acid composition in obese and lean pigs
Obese and lean type pig breeds exhibit differences in their fat deposits and fatty acid composition. Here, we compared the effect of genome-wide DNA methylation on fatty acid metabolism between Landrace pigs (LP, leaner) and Rongchang pigs (RP, fatty). We found that LP backfat (LBF) had a higher pol...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5056348/ https://www.ncbi.nlm.nih.gov/pubmed/27721392 http://dx.doi.org/10.1038/srep35063 |
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author | Zhang, Shunhua Shen, Linyuan Xia, Yudong Yang, Qiong Li, Xuewei Tang, Guoqing Jiang, Yanzhi Wang, Jinyong Li, Mingzhou Zhu, Li |
author_facet | Zhang, Shunhua Shen, Linyuan Xia, Yudong Yang, Qiong Li, Xuewei Tang, Guoqing Jiang, Yanzhi Wang, Jinyong Li, Mingzhou Zhu, Li |
author_sort | Zhang, Shunhua |
collection | PubMed |
description | Obese and lean type pig breeds exhibit differences in their fat deposits and fatty acid composition. Here, we compared the effect of genome-wide DNA methylation on fatty acid metabolism between Landrace pigs (LP, leaner) and Rongchang pigs (RP, fatty). We found that LP backfat (LBF) had a higher polyunsaturated fatty acid content but a lower adipocyte volume than RP backfat (RBF). LBF exhibited higher global DNA methylation levels at the genome level than RBF. A total of 483 differentially methylated regions (DMRs) were located in promoter regions, mainly affecting olfactory and sensory activity and lipid metabolism. In LBF, the promoters of genes related to ATPase activity had significantly stronger methylation. This fact may suggest lower energy metabolism levels, which may result in less efficient lipid synthesis in LBF. Furthermore, we identified a DMR in the miR-4335 and miR-378 promoters and validated their methylation status by bisulfite sequencing PCR. The hypermethylation of the promoters of miR-4335 and miR-378 in LBF and the resulting silencing of the target genes may result in LBF’s low content in saturated fatty acids and fat deposition capacity. This study provides a solid basis for exploring the epigenetic mechanisms affecting fat deposition and fatty acid composition. |
format | Online Article Text |
id | pubmed-5056348 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-50563482016-10-19 DNA methylation landscape of fat deposits and fatty acid composition in obese and lean pigs Zhang, Shunhua Shen, Linyuan Xia, Yudong Yang, Qiong Li, Xuewei Tang, Guoqing Jiang, Yanzhi Wang, Jinyong Li, Mingzhou Zhu, Li Sci Rep Article Obese and lean type pig breeds exhibit differences in their fat deposits and fatty acid composition. Here, we compared the effect of genome-wide DNA methylation on fatty acid metabolism between Landrace pigs (LP, leaner) and Rongchang pigs (RP, fatty). We found that LP backfat (LBF) had a higher polyunsaturated fatty acid content but a lower adipocyte volume than RP backfat (RBF). LBF exhibited higher global DNA methylation levels at the genome level than RBF. A total of 483 differentially methylated regions (DMRs) were located in promoter regions, mainly affecting olfactory and sensory activity and lipid metabolism. In LBF, the promoters of genes related to ATPase activity had significantly stronger methylation. This fact may suggest lower energy metabolism levels, which may result in less efficient lipid synthesis in LBF. Furthermore, we identified a DMR in the miR-4335 and miR-378 promoters and validated their methylation status by bisulfite sequencing PCR. The hypermethylation of the promoters of miR-4335 and miR-378 in LBF and the resulting silencing of the target genes may result in LBF’s low content in saturated fatty acids and fat deposition capacity. This study provides a solid basis for exploring the epigenetic mechanisms affecting fat deposition and fatty acid composition. Nature Publishing Group 2016-10-10 /pmc/articles/PMC5056348/ /pubmed/27721392 http://dx.doi.org/10.1038/srep35063 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Zhang, Shunhua Shen, Linyuan Xia, Yudong Yang, Qiong Li, Xuewei Tang, Guoqing Jiang, Yanzhi Wang, Jinyong Li, Mingzhou Zhu, Li DNA methylation landscape of fat deposits and fatty acid composition in obese and lean pigs |
title | DNA methylation landscape of fat deposits and fatty acid composition in obese and lean pigs |
title_full | DNA methylation landscape of fat deposits and fatty acid composition in obese and lean pigs |
title_fullStr | DNA methylation landscape of fat deposits and fatty acid composition in obese and lean pigs |
title_full_unstemmed | DNA methylation landscape of fat deposits and fatty acid composition in obese and lean pigs |
title_short | DNA methylation landscape of fat deposits and fatty acid composition in obese and lean pigs |
title_sort | dna methylation landscape of fat deposits and fatty acid composition in obese and lean pigs |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5056348/ https://www.ncbi.nlm.nih.gov/pubmed/27721392 http://dx.doi.org/10.1038/srep35063 |
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