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Potential differentiation ability of gingiva originated human mesenchymal stem cell in the presence of tacrolimus
The aim of the present study is to evaluate the potential differentiation ability of gingiva originated human mesenchymal stem cell in the presence of tacrolimus. Tacrolimus-loaded poly(lactic-co-glycolic acid) microspheres were prepared using electrospraying technique. In vitro release study of tac...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5056516/ https://www.ncbi.nlm.nih.gov/pubmed/27721434 http://dx.doi.org/10.1038/srep34910 |
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author | Ha, Dong-Ho Pathak, Shiva Yong, Chul Soon Kim, Jong Oh Jeong, Jee-Heon Park, Jun-Beom |
author_facet | Ha, Dong-Ho Pathak, Shiva Yong, Chul Soon Kim, Jong Oh Jeong, Jee-Heon Park, Jun-Beom |
author_sort | Ha, Dong-Ho |
collection | PubMed |
description | The aim of the present study is to evaluate the potential differentiation ability of gingiva originated human mesenchymal stem cell in the presence of tacrolimus. Tacrolimus-loaded poly(lactic-co-glycolic acid) microspheres were prepared using electrospraying technique. In vitro release study of tacrolimus-loaded poly(lactic-co-glycolic acid) microspheres was performed in phosphate-buffered saline (pH 7.4). Gingiva-derived stem cells were isolated and incubated with tacrolimus or tacrolimus-loaded microspheres. Release study of the microspheres revealed prolonged release profiles of tacrolimus without any significant initial burst release. The microsphere itself did not affect the morphology of the mesenchymal stem cells, and cell morphology was retained after incubation with microspheres loaded with tacrolimus at 1 μg/mL to 10 μg/mL. Cultures grown in the presence of microspheres loaded with tacrolimus at 1 μg/mL showed the highest mineralization. Alkaline phosphatase activity increased with an increase in incubation time. The highest expression of pSmad1/5 was achieved in the group receiving tacrolimus 0.1 μg/mL every third day, and the highest expression of osteocalcin was achieved in the group receiving 1 μg/mL every third day. Biodegradable poly(lactic-co-glycolic acid)-based microspheres loaded with tacrolimus promoted mineralization. Microspheres loaded with tacrolimus may be applied for increased osteoblastic differentiation. |
format | Online Article Text |
id | pubmed-5056516 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-50565162016-10-19 Potential differentiation ability of gingiva originated human mesenchymal stem cell in the presence of tacrolimus Ha, Dong-Ho Pathak, Shiva Yong, Chul Soon Kim, Jong Oh Jeong, Jee-Heon Park, Jun-Beom Sci Rep Article The aim of the present study is to evaluate the potential differentiation ability of gingiva originated human mesenchymal stem cell in the presence of tacrolimus. Tacrolimus-loaded poly(lactic-co-glycolic acid) microspheres were prepared using electrospraying technique. In vitro release study of tacrolimus-loaded poly(lactic-co-glycolic acid) microspheres was performed in phosphate-buffered saline (pH 7.4). Gingiva-derived stem cells were isolated and incubated with tacrolimus or tacrolimus-loaded microspheres. Release study of the microspheres revealed prolonged release profiles of tacrolimus without any significant initial burst release. The microsphere itself did not affect the morphology of the mesenchymal stem cells, and cell morphology was retained after incubation with microspheres loaded with tacrolimus at 1 μg/mL to 10 μg/mL. Cultures grown in the presence of microspheres loaded with tacrolimus at 1 μg/mL showed the highest mineralization. Alkaline phosphatase activity increased with an increase in incubation time. The highest expression of pSmad1/5 was achieved in the group receiving tacrolimus 0.1 μg/mL every third day, and the highest expression of osteocalcin was achieved in the group receiving 1 μg/mL every third day. Biodegradable poly(lactic-co-glycolic acid)-based microspheres loaded with tacrolimus promoted mineralization. Microspheres loaded with tacrolimus may be applied for increased osteoblastic differentiation. Nature Publishing Group 2016-10-10 /pmc/articles/PMC5056516/ /pubmed/27721434 http://dx.doi.org/10.1038/srep34910 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Ha, Dong-Ho Pathak, Shiva Yong, Chul Soon Kim, Jong Oh Jeong, Jee-Heon Park, Jun-Beom Potential differentiation ability of gingiva originated human mesenchymal stem cell in the presence of tacrolimus |
title | Potential differentiation ability of gingiva originated human mesenchymal stem cell in the presence of tacrolimus |
title_full | Potential differentiation ability of gingiva originated human mesenchymal stem cell in the presence of tacrolimus |
title_fullStr | Potential differentiation ability of gingiva originated human mesenchymal stem cell in the presence of tacrolimus |
title_full_unstemmed | Potential differentiation ability of gingiva originated human mesenchymal stem cell in the presence of tacrolimus |
title_short | Potential differentiation ability of gingiva originated human mesenchymal stem cell in the presence of tacrolimus |
title_sort | potential differentiation ability of gingiva originated human mesenchymal stem cell in the presence of tacrolimus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5056516/ https://www.ncbi.nlm.nih.gov/pubmed/27721434 http://dx.doi.org/10.1038/srep34910 |
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