A Tale of Tails: Dissecting the Enhancing Effect of Tailed Primers in Real-Time PCR

Non-specific tail sequences are often added to the 5’-terminus of primers to improve the robustness and overall performance of diagnostic assays. Despite the widespread use of tailed primers, the underlying working mechanism is not well understood. To address this problem, we conducted a detailed in...

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Detalles Bibliográficos
Autores principales: Vandenbussche, Frank, Mathijs, Elisabeth, Lefebvre, David, De Clercq, Kris, Van Borm, Steven
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5056738/
https://www.ncbi.nlm.nih.gov/pubmed/27723800
http://dx.doi.org/10.1371/journal.pone.0164463

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5056738/
https://www.ncbi.nlm.nih.gov/pubmed/27723800
http://dx.doi.org/10.1371/journal.pone.0164463

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