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Assessment of collagen antibody-induced arthritis in BALB/c mice using bioimaging analysis and histopathological examination

The aim of this study was to examine the therapeutic potential of sulfasalazine and prednisolone in a mouse collagen antibody-induced arthritis (CAIA) model. Twenty-five male BALB/c mice were randomly divided into five groups: group 1 (G1): control, group 2 (G2): probe control, group 3 (G3): CAIA, g...

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Autores principales: Sim, Joo Hye, Lee, Won Kil, Lee, Yun Seok, Kang, Jin Seok
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Association for Laboratory Animal Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5057001/
https://www.ncbi.nlm.nih.gov/pubmed/27729929
http://dx.doi.org/10.5625/lar.2016.32.3.135
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author Sim, Joo Hye
Lee, Won Kil
Lee, Yun Seok
Kang, Jin Seok
author_facet Sim, Joo Hye
Lee, Won Kil
Lee, Yun Seok
Kang, Jin Seok
author_sort Sim, Joo Hye
collection PubMed
description The aim of this study was to examine the therapeutic potential of sulfasalazine and prednisolone in a mouse collagen antibody-induced arthritis (CAIA) model. Twenty-five male BALB/c mice were randomly divided into five groups: group 1 (G1): control, group 2 (G2): probe control, group 3 (G3): CAIA, group 4 (G4): CAIA+sulfasalazine (10 mg/kg, oral), and group 5 (G5): CAIA+prednisolone (100 mg/kg, oral). Fluorescence bioimaging was performed in vivo 24 and 48 h after treatment with a fluorescence probe (OsteoSense® 680 EX), and all mice were sacrificed. The hind knee joints were fixed in 10% neutral phosphate-buffered formalin, and micro-computed tomography (micro-CT) and histopathological analyses were performed. The paw thickness increased in a time-dependent manner in G3 mice, but trended toward a decrease in both G4 and G5 mice. Fluorescence intensity increased in G3 mice at 24 and 48 h after fluorescence probe treatment, but the fluorescence intensity in G4 and G5 mice was lower than that in G3. Micro-CT analyses showed that the joint surfaces of G3 mice had a rough and irregular articular appearance, but the occurrence of these irregularities was lower in G4 and G5. Hematoxylin and eosin and Safranin O-fast green staining confirmed that destruction of the cartilage and bony structures, synovial hyperplasia, and inflammatory cell infiltration all occurred in G3, and that the occurrence of these phenomena was lower in G4 and G5 than in G3. Taken together, these results suggest that sulfasalazine and prednisolone can reduce acute rheumatoid arthritis in mice.
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spelling pubmed-50570012016-10-11 Assessment of collagen antibody-induced arthritis in BALB/c mice using bioimaging analysis and histopathological examination Sim, Joo Hye Lee, Won Kil Lee, Yun Seok Kang, Jin Seok Lab Anim Res Original Article The aim of this study was to examine the therapeutic potential of sulfasalazine and prednisolone in a mouse collagen antibody-induced arthritis (CAIA) model. Twenty-five male BALB/c mice were randomly divided into five groups: group 1 (G1): control, group 2 (G2): probe control, group 3 (G3): CAIA, group 4 (G4): CAIA+sulfasalazine (10 mg/kg, oral), and group 5 (G5): CAIA+prednisolone (100 mg/kg, oral). Fluorescence bioimaging was performed in vivo 24 and 48 h after treatment with a fluorescence probe (OsteoSense® 680 EX), and all mice were sacrificed. The hind knee joints were fixed in 10% neutral phosphate-buffered formalin, and micro-computed tomography (micro-CT) and histopathological analyses were performed. The paw thickness increased in a time-dependent manner in G3 mice, but trended toward a decrease in both G4 and G5 mice. Fluorescence intensity increased in G3 mice at 24 and 48 h after fluorescence probe treatment, but the fluorescence intensity in G4 and G5 mice was lower than that in G3. Micro-CT analyses showed that the joint surfaces of G3 mice had a rough and irregular articular appearance, but the occurrence of these irregularities was lower in G4 and G5. Hematoxylin and eosin and Safranin O-fast green staining confirmed that destruction of the cartilage and bony structures, synovial hyperplasia, and inflammatory cell infiltration all occurred in G3, and that the occurrence of these phenomena was lower in G4 and G5 than in G3. Taken together, these results suggest that sulfasalazine and prednisolone can reduce acute rheumatoid arthritis in mice. Korean Association for Laboratory Animal Science 2016-09 2016-09-30 /pmc/articles/PMC5057001/ /pubmed/27729929 http://dx.doi.org/10.5625/lar.2016.32.3.135 Text en Copyright © 2016 Korean Association for Laboratory Animal Science http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Sim, Joo Hye
Lee, Won Kil
Lee, Yun Seok
Kang, Jin Seok
Assessment of collagen antibody-induced arthritis in BALB/c mice using bioimaging analysis and histopathological examination
title Assessment of collagen antibody-induced arthritis in BALB/c mice using bioimaging analysis and histopathological examination
title_full Assessment of collagen antibody-induced arthritis in BALB/c mice using bioimaging analysis and histopathological examination
title_fullStr Assessment of collagen antibody-induced arthritis in BALB/c mice using bioimaging analysis and histopathological examination
title_full_unstemmed Assessment of collagen antibody-induced arthritis in BALB/c mice using bioimaging analysis and histopathological examination
title_short Assessment of collagen antibody-induced arthritis in BALB/c mice using bioimaging analysis and histopathological examination
title_sort assessment of collagen antibody-induced arthritis in balb/c mice using bioimaging analysis and histopathological examination
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5057001/
https://www.ncbi.nlm.nih.gov/pubmed/27729929
http://dx.doi.org/10.5625/lar.2016.32.3.135
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