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Seroprevalence of contagious ecthyma in goats of Assam: An analysis by indirect enzyme-linked immunosorbent assay

AIM: The objective of this study was to screen the prevalence of contagious ecthyma (CE) among the goat population of Assam owing to its high prevalence rate. MATERIALS AND METHODS: In this study, a total of 231 serum samples were collected from 12 districts of Assam during September 2013 to July 20...

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Autores principales: Bora, Mousumi, Bora, Durlav Prasad, Barman, Nagendra Nath, Borah, Biswajyoti, Das, Sutopa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Veterinary World 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5057025/
https://www.ncbi.nlm.nih.gov/pubmed/27733808
http://dx.doi.org/10.14202/vetworld.2016.1028-1033
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author Bora, Mousumi
Bora, Durlav Prasad
Barman, Nagendra Nath
Borah, Biswajyoti
Das, Sutopa
author_facet Bora, Mousumi
Bora, Durlav Prasad
Barman, Nagendra Nath
Borah, Biswajyoti
Das, Sutopa
author_sort Bora, Mousumi
collection PubMed
description AIM: The objective of this study was to screen the prevalence of contagious ecthyma (CE) among the goat population of Assam owing to its high prevalence rate. MATERIALS AND METHODS: In this study, a total of 231 serum samples were collected from 12 districts of Assam during September 2013 to July 2014. The serum samples were tested for the presence of antibodies against Orf virus (ORFV) by indirect enzyme-linked immunosorbent assay (ELISA). Indirect ELISA was standardized using purified Orf reference virus produced in bulk in primary lamb testes cells. RESULTS: Studies on seroprevalence showed 76.62% of goats were seropositive. The total number of animals were divided into different age groups starting from 0-2 months, 2-4 months, 4-6 months, and above 8 months and accordingly highest prevalence of antibodies against ORFV was recorded in the age-group above 8 months of age. Significantly, lower rates of infection were observed in goats of age group 2-4 months. This study recorded that seropositivity from naturally infected animals and in contact apparently healthy animals to be 53.67% and 46.32%, respectively. CONCLUSION: The results indicated that CE is a prevalent infection in goats of Assam, and the healthy population is at increased risk of infection.
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spelling pubmed-50570252016-10-12 Seroprevalence of contagious ecthyma in goats of Assam: An analysis by indirect enzyme-linked immunosorbent assay Bora, Mousumi Bora, Durlav Prasad Barman, Nagendra Nath Borah, Biswajyoti Das, Sutopa Vet World Research Article AIM: The objective of this study was to screen the prevalence of contagious ecthyma (CE) among the goat population of Assam owing to its high prevalence rate. MATERIALS AND METHODS: In this study, a total of 231 serum samples were collected from 12 districts of Assam during September 2013 to July 2014. The serum samples were tested for the presence of antibodies against Orf virus (ORFV) by indirect enzyme-linked immunosorbent assay (ELISA). Indirect ELISA was standardized using purified Orf reference virus produced in bulk in primary lamb testes cells. RESULTS: Studies on seroprevalence showed 76.62% of goats were seropositive. The total number of animals were divided into different age groups starting from 0-2 months, 2-4 months, 4-6 months, and above 8 months and accordingly highest prevalence of antibodies against ORFV was recorded in the age-group above 8 months of age. Significantly, lower rates of infection were observed in goats of age group 2-4 months. This study recorded that seropositivity from naturally infected animals and in contact apparently healthy animals to be 53.67% and 46.32%, respectively. CONCLUSION: The results indicated that CE is a prevalent infection in goats of Assam, and the healthy population is at increased risk of infection. Veterinary World 2016-09 2016-09-28 /pmc/articles/PMC5057025/ /pubmed/27733808 http://dx.doi.org/10.14202/vetworld.2016.1028-1033 Text en Copyright: © Bora, et al. http://creativecommons.org/licenses/by/4.0 Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Bora, Mousumi
Bora, Durlav Prasad
Barman, Nagendra Nath
Borah, Biswajyoti
Das, Sutopa
Seroprevalence of contagious ecthyma in goats of Assam: An analysis by indirect enzyme-linked immunosorbent assay
title Seroprevalence of contagious ecthyma in goats of Assam: An analysis by indirect enzyme-linked immunosorbent assay
title_full Seroprevalence of contagious ecthyma in goats of Assam: An analysis by indirect enzyme-linked immunosorbent assay
title_fullStr Seroprevalence of contagious ecthyma in goats of Assam: An analysis by indirect enzyme-linked immunosorbent assay
title_full_unstemmed Seroprevalence of contagious ecthyma in goats of Assam: An analysis by indirect enzyme-linked immunosorbent assay
title_short Seroprevalence of contagious ecthyma in goats of Assam: An analysis by indirect enzyme-linked immunosorbent assay
title_sort seroprevalence of contagious ecthyma in goats of assam: an analysis by indirect enzyme-linked immunosorbent assay
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5057025/
https://www.ncbi.nlm.nih.gov/pubmed/27733808
http://dx.doi.org/10.14202/vetworld.2016.1028-1033
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