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Formation of gold nanoparticles by glycolipids of Lactobacillus casei

Gold nanoparticles have particular properties distinct from those of bulk gold crystals, and such nanoparticles are used in various applications in optics, catalysis, and drug delivery. Many reports on microbial synthesis of gold nanoparticles have appeared. However, the molecular details (reduction...

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Autores principales: Kikuchi, Fumiya, Kato, Yugo, Furihata, Kazuo, Kogure, Toshihiro, Imura, Yuki, Yoshimura, Etsuro, Suzuki, Michio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5057074/
https://www.ncbi.nlm.nih.gov/pubmed/27725710
http://dx.doi.org/10.1038/srep34626
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author Kikuchi, Fumiya
Kato, Yugo
Furihata, Kazuo
Kogure, Toshihiro
Imura, Yuki
Yoshimura, Etsuro
Suzuki, Michio
author_facet Kikuchi, Fumiya
Kato, Yugo
Furihata, Kazuo
Kogure, Toshihiro
Imura, Yuki
Yoshimura, Etsuro
Suzuki, Michio
author_sort Kikuchi, Fumiya
collection PubMed
description Gold nanoparticles have particular properties distinct from those of bulk gold crystals, and such nanoparticles are used in various applications in optics, catalysis, and drug delivery. Many reports on microbial synthesis of gold nanoparticles have appeared. However, the molecular details (reduction and dispersion) of such synthesis remain unclear. In the present study, we studied gold nanoparticle synthesis by Lactobacillus casei. A comparison of L. casei components before and after addition of an auric acid solution showed that the level of unsaturated lipids decreased significantly after addition. NMR and mass spectrum analysis showed that the levels of diglycosyldiacylglycerol (DGDG) and triglycosyldiacylglycerol (TGDG) bearing unsaturated fatty acids were much reduced after formation of gold nanoparticles. DGDG purified from L. casei induced the synthesis of gold nanoparticles in vitro. These results suggested that glycolipids, such as DGDG, play important roles in reducing Au(III) to Au(0) and in ensuring that the nanoparticles synthesized remain small in size. Our work will lead to the development of novel, efficient methods by which gold nanoparticles may be produced by, and accumulated within, microorganisms.
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spelling pubmed-50570742016-10-24 Formation of gold nanoparticles by glycolipids of Lactobacillus casei Kikuchi, Fumiya Kato, Yugo Furihata, Kazuo Kogure, Toshihiro Imura, Yuki Yoshimura, Etsuro Suzuki, Michio Sci Rep Article Gold nanoparticles have particular properties distinct from those of bulk gold crystals, and such nanoparticles are used in various applications in optics, catalysis, and drug delivery. Many reports on microbial synthesis of gold nanoparticles have appeared. However, the molecular details (reduction and dispersion) of such synthesis remain unclear. In the present study, we studied gold nanoparticle synthesis by Lactobacillus casei. A comparison of L. casei components before and after addition of an auric acid solution showed that the level of unsaturated lipids decreased significantly after addition. NMR and mass spectrum analysis showed that the levels of diglycosyldiacylglycerol (DGDG) and triglycosyldiacylglycerol (TGDG) bearing unsaturated fatty acids were much reduced after formation of gold nanoparticles. DGDG purified from L. casei induced the synthesis of gold nanoparticles in vitro. These results suggested that glycolipids, such as DGDG, play important roles in reducing Au(III) to Au(0) and in ensuring that the nanoparticles synthesized remain small in size. Our work will lead to the development of novel, efficient methods by which gold nanoparticles may be produced by, and accumulated within, microorganisms. Nature Publishing Group 2016-10-11 /pmc/articles/PMC5057074/ /pubmed/27725710 http://dx.doi.org/10.1038/srep34626 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Kikuchi, Fumiya
Kato, Yugo
Furihata, Kazuo
Kogure, Toshihiro
Imura, Yuki
Yoshimura, Etsuro
Suzuki, Michio
Formation of gold nanoparticles by glycolipids of Lactobacillus casei
title Formation of gold nanoparticles by glycolipids of Lactobacillus casei
title_full Formation of gold nanoparticles by glycolipids of Lactobacillus casei
title_fullStr Formation of gold nanoparticles by glycolipids of Lactobacillus casei
title_full_unstemmed Formation of gold nanoparticles by glycolipids of Lactobacillus casei
title_short Formation of gold nanoparticles by glycolipids of Lactobacillus casei
title_sort formation of gold nanoparticles by glycolipids of lactobacillus casei
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5057074/
https://www.ncbi.nlm.nih.gov/pubmed/27725710
http://dx.doi.org/10.1038/srep34626
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