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Functional visualization and disruption of targeted genes using CRISPR/Cas9-mediated eGFP reporter integration in zebrafish

The CRISPR/Cas9 complex, which is composed of a guide RNA (gRNA) and the Cas9 nuclease, is useful for carrying out genome modifications in various organisms. Recently, the CRISPR/Cas9-mediated locus-specific integration of a reporter, which contains the Mbait sequence targeted using Mbait-gRNA, the...

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Detalles Bibliográficos
Autores principales:	Ota, Satoshi, Taimatsu, Kiyohito, Yanagi, Kanoko, Namiki, Tomohiro, Ohga, Rie, Higashijima, Shin-ichi, Kawahara, Atsuo
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2016
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5057081/ https://www.ncbi.nlm.nih.gov/pubmed/27725766 http://dx.doi.org/10.1038/srep34991

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