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HIC1 controls cellular- and HIV-1- gene transcription via interactions with CTIP2 and HMGA1

Among many cellular transcriptional regulators, Bcl11b/CTIP2 and HGMA1 have been described to control the establishment and the persistence of HIV-1 latency in microglial cells, the main viral reservoir in the brain. In this present work, we identify and characterize a transcription factor i.e. HIC1...

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Autores principales: Le Douce, Valentin, Forouzanfar, Faezeh, Eilebrecht, Sebastian, Van Driessche, Benoit, Ait-Ammar, Amina, Verdikt, Roxane, Kurashige, Yoshihito, Marban, Céline, Gautier, Virginie, Candolfi, Ermanno, Benecke, Arndt G., Van Lint, Carine, Rohr, Olivier, Schwartz, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5057145/
https://www.ncbi.nlm.nih.gov/pubmed/27725726
http://dx.doi.org/10.1038/srep34920
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author Le Douce, Valentin
Forouzanfar, Faezeh
Eilebrecht, Sebastian
Van Driessche, Benoit
Ait-Ammar, Amina
Verdikt, Roxane
Kurashige, Yoshihito
Marban, Céline
Gautier, Virginie
Candolfi, Ermanno
Benecke, Arndt G.
Van Lint, Carine
Rohr, Olivier
Schwartz, Christian
author_facet Le Douce, Valentin
Forouzanfar, Faezeh
Eilebrecht, Sebastian
Van Driessche, Benoit
Ait-Ammar, Amina
Verdikt, Roxane
Kurashige, Yoshihito
Marban, Céline
Gautier, Virginie
Candolfi, Ermanno
Benecke, Arndt G.
Van Lint, Carine
Rohr, Olivier
Schwartz, Christian
author_sort Le Douce, Valentin
collection PubMed
description Among many cellular transcriptional regulators, Bcl11b/CTIP2 and HGMA1 have been described to control the establishment and the persistence of HIV-1 latency in microglial cells, the main viral reservoir in the brain. In this present work, we identify and characterize a transcription factor i.e. HIC1, which physically interacts with both Bcl11b/CTIP2 and HMGA1 to co-regulate specific subsets of cellular genes and the viral HIV-1 gene. Our results suggest that HIC1 represses Tat dependent HIV-1 transcription. Interestingly, this repression of Tat function is linked to HIC1 K314 acetylation status and to SIRT1 deacetylase activity. Finally, we show that HIC1 interacts and cooperates with HGMA1 to regulate Tat dependent HIV-1 transcription. Our results also suggest that HIC1 repression of Tat function happens in a TAR dependent manner and that this TAR element may serve as HIC1 reservoir at the viral promoter to facilitate HIC1/TAT interaction.
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spelling pubmed-50571452016-10-24 HIC1 controls cellular- and HIV-1- gene transcription via interactions with CTIP2 and HMGA1 Le Douce, Valentin Forouzanfar, Faezeh Eilebrecht, Sebastian Van Driessche, Benoit Ait-Ammar, Amina Verdikt, Roxane Kurashige, Yoshihito Marban, Céline Gautier, Virginie Candolfi, Ermanno Benecke, Arndt G. Van Lint, Carine Rohr, Olivier Schwartz, Christian Sci Rep Article Among many cellular transcriptional regulators, Bcl11b/CTIP2 and HGMA1 have been described to control the establishment and the persistence of HIV-1 latency in microglial cells, the main viral reservoir in the brain. In this present work, we identify and characterize a transcription factor i.e. HIC1, which physically interacts with both Bcl11b/CTIP2 and HMGA1 to co-regulate specific subsets of cellular genes and the viral HIV-1 gene. Our results suggest that HIC1 represses Tat dependent HIV-1 transcription. Interestingly, this repression of Tat function is linked to HIC1 K314 acetylation status and to SIRT1 deacetylase activity. Finally, we show that HIC1 interacts and cooperates with HGMA1 to regulate Tat dependent HIV-1 transcription. Our results also suggest that HIC1 repression of Tat function happens in a TAR dependent manner and that this TAR element may serve as HIC1 reservoir at the viral promoter to facilitate HIC1/TAT interaction. Nature Publishing Group 2016-10-11 /pmc/articles/PMC5057145/ /pubmed/27725726 http://dx.doi.org/10.1038/srep34920 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Le Douce, Valentin
Forouzanfar, Faezeh
Eilebrecht, Sebastian
Van Driessche, Benoit
Ait-Ammar, Amina
Verdikt, Roxane
Kurashige, Yoshihito
Marban, Céline
Gautier, Virginie
Candolfi, Ermanno
Benecke, Arndt G.
Van Lint, Carine
Rohr, Olivier
Schwartz, Christian
HIC1 controls cellular- and HIV-1- gene transcription via interactions with CTIP2 and HMGA1
title HIC1 controls cellular- and HIV-1- gene transcription via interactions with CTIP2 and HMGA1
title_full HIC1 controls cellular- and HIV-1- gene transcription via interactions with CTIP2 and HMGA1
title_fullStr HIC1 controls cellular- and HIV-1- gene transcription via interactions with CTIP2 and HMGA1
title_full_unstemmed HIC1 controls cellular- and HIV-1- gene transcription via interactions with CTIP2 and HMGA1
title_short HIC1 controls cellular- and HIV-1- gene transcription via interactions with CTIP2 and HMGA1
title_sort hic1 controls cellular- and hiv-1- gene transcription via interactions with ctip2 and hmga1
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5057145/
https://www.ncbi.nlm.nih.gov/pubmed/27725726
http://dx.doi.org/10.1038/srep34920
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