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Mechanisms underlying heterologous skin scaffold-mediated tissue remodeling

Biocompatibility of two newly developed porcine skin scaffolds was assessed after 3, 14, 21 and 90 days of implantation in rats. Both scaffolds showed absence of cells, preservation of ECM and mechanical properties comparable to non-decellularised skin before implantation. Host cell infiltration was...

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Autores principales: Mimura, Kallyne K. O., Moraes, Andréia R., Miranda, Aline C., Greco, Rebecca, Ansari, Tahera, Sibbons, Paul, Greco, Karin V., Oliani, Sonia M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5057165/
https://www.ncbi.nlm.nih.gov/pubmed/27725772
http://dx.doi.org/10.1038/srep35074
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author Mimura, Kallyne K. O.
Moraes, Andréia R.
Miranda, Aline C.
Greco, Rebecca
Ansari, Tahera
Sibbons, Paul
Greco, Karin V.
Oliani, Sonia M.
author_facet Mimura, Kallyne K. O.
Moraes, Andréia R.
Miranda, Aline C.
Greco, Rebecca
Ansari, Tahera
Sibbons, Paul
Greco, Karin V.
Oliani, Sonia M.
author_sort Mimura, Kallyne K. O.
collection PubMed
description Biocompatibility of two newly developed porcine skin scaffolds was assessed after 3, 14, 21 and 90 days of implantation in rats. Both scaffolds showed absence of cells, preservation of ECM and mechanical properties comparable to non-decellularised skin before implantation. Host cell infiltration was much prominent on both scaffolds when compared to Permacol (surgical control). At day 3, the grafts were surrounded by polymorphonuclear cells, which were replaced by a notable number of IL-6-positive cells at day 14. Simultaneously, the number of pro-inflammatory M1-macrophage was enhanced. Interestingly, a predominant pro-remodeling M2 response, with newly formed vessels, myofibroblasts activation and a shift on the type of collagen expression was sequentially delayed (around 21 days). The gene expression of some trophic factors involved in tissue remodeling was congruent with the cellular events. Our findings suggested that the responsiveness of macrophages after non-crosslinked skin scaffolds implantation seemed to intimately affect various cell responses and molecular events; and this range of mutually reinforcing actions was predictive of a positive tissue remodeling that was essential for the long-standing success of the implants. Furthermore, our study indicates that non-crosslinked biologic scaffold implantation is biocompatible to the host tissue and somehow underlying molecular events involved in tissue repair.
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spelling pubmed-50571652016-10-24 Mechanisms underlying heterologous skin scaffold-mediated tissue remodeling Mimura, Kallyne K. O. Moraes, Andréia R. Miranda, Aline C. Greco, Rebecca Ansari, Tahera Sibbons, Paul Greco, Karin V. Oliani, Sonia M. Sci Rep Article Biocompatibility of two newly developed porcine skin scaffolds was assessed after 3, 14, 21 and 90 days of implantation in rats. Both scaffolds showed absence of cells, preservation of ECM and mechanical properties comparable to non-decellularised skin before implantation. Host cell infiltration was much prominent on both scaffolds when compared to Permacol (surgical control). At day 3, the grafts were surrounded by polymorphonuclear cells, which were replaced by a notable number of IL-6-positive cells at day 14. Simultaneously, the number of pro-inflammatory M1-macrophage was enhanced. Interestingly, a predominant pro-remodeling M2 response, with newly formed vessels, myofibroblasts activation and a shift on the type of collagen expression was sequentially delayed (around 21 days). The gene expression of some trophic factors involved in tissue remodeling was congruent with the cellular events. Our findings suggested that the responsiveness of macrophages after non-crosslinked skin scaffolds implantation seemed to intimately affect various cell responses and molecular events; and this range of mutually reinforcing actions was predictive of a positive tissue remodeling that was essential for the long-standing success of the implants. Furthermore, our study indicates that non-crosslinked biologic scaffold implantation is biocompatible to the host tissue and somehow underlying molecular events involved in tissue repair. Nature Publishing Group 2016-10-11 /pmc/articles/PMC5057165/ /pubmed/27725772 http://dx.doi.org/10.1038/srep35074 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Mimura, Kallyne K. O.
Moraes, Andréia R.
Miranda, Aline C.
Greco, Rebecca
Ansari, Tahera
Sibbons, Paul
Greco, Karin V.
Oliani, Sonia M.
Mechanisms underlying heterologous skin scaffold-mediated tissue remodeling
title Mechanisms underlying heterologous skin scaffold-mediated tissue remodeling
title_full Mechanisms underlying heterologous skin scaffold-mediated tissue remodeling
title_fullStr Mechanisms underlying heterologous skin scaffold-mediated tissue remodeling
title_full_unstemmed Mechanisms underlying heterologous skin scaffold-mediated tissue remodeling
title_short Mechanisms underlying heterologous skin scaffold-mediated tissue remodeling
title_sort mechanisms underlying heterologous skin scaffold-mediated tissue remodeling
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5057165/
https://www.ncbi.nlm.nih.gov/pubmed/27725772
http://dx.doi.org/10.1038/srep35074
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