A capillary-based multiplexed isothermal nucleic acid-based test for sexually transmitted diseases in patients

We demonstrate a multiplexed loop mediated isothermal amplification (LAMP) assay for infectious disease diagnostics, where the analytical process flow of target pathogens genomic DNA is performed manually by moving magnetic beads through a series of plugs in a capillary. Heat is provided by a water...

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Detalles Bibliográficos
Autores principales: Xu, Gaolian, Zhao, Hang, Cooper, Jonathan M., Reboud, Julien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2016
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5058349/
https://www.ncbi.nlm.nih.gov/pubmed/27722490
http://dx.doi.org/10.1039/c6cc05679b
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author Xu, Gaolian
Zhao, Hang
Cooper, Jonathan M.
Reboud, Julien
author_facet Xu, Gaolian
Zhao, Hang
Cooper, Jonathan M.
Reboud, Julien
author_sort Xu, Gaolian
collection PubMed
description We demonstrate a multiplexed loop mediated isothermal amplification (LAMP) assay for infectious disease diagnostics, where the analytical process flow of target pathogens genomic DNA is performed manually by moving magnetic beads through a series of plugs in a capillary. Heat is provided by a water bath and the results are read by the naked eye, enabling applications in low resource settings.
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spelling pubmed-50583492016-10-12 A capillary-based multiplexed isothermal nucleic acid-based test for sexually transmitted diseases in patients Xu, Gaolian Zhao, Hang Cooper, Jonathan M. Reboud, Julien Chem Commun (Camb) Chemistry We demonstrate a multiplexed loop mediated isothermal amplification (LAMP) assay for infectious disease diagnostics, where the analytical process flow of target pathogens genomic DNA is performed manually by moving magnetic beads through a series of plugs in a capillary. Heat is provided by a water bath and the results are read by the naked eye, enabling applications in low resource settings. Royal Society of Chemistry 2016-10-21 2016-09-08 /pmc/articles/PMC5058349/ /pubmed/27722490 http://dx.doi.org/10.1039/c6cc05679b Text en This journal is © The Royal Society of Chemistry 2016 http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution 3.0 Unported License (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Chemistry
Xu, Gaolian
Zhao, Hang
Cooper, Jonathan M.
Reboud, Julien
A capillary-based multiplexed isothermal nucleic acid-based test for sexually transmitted diseases in patients
title A capillary-based multiplexed isothermal nucleic acid-based test for sexually transmitted diseases in patients
title_full A capillary-based multiplexed isothermal nucleic acid-based test for sexually transmitted diseases in patients
title_fullStr A capillary-based multiplexed isothermal nucleic acid-based test for sexually transmitted diseases in patients
title_full_unstemmed A capillary-based multiplexed isothermal nucleic acid-based test for sexually transmitted diseases in patients
title_short A capillary-based multiplexed isothermal nucleic acid-based test for sexually transmitted diseases in patients
title_sort capillary-based multiplexed isothermal nucleic acid-based test for sexually transmitted diseases in patients
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5058349/
https://www.ncbi.nlm.nih.gov/pubmed/27722490
http://dx.doi.org/10.1039/c6cc05679b
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