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miR-675-5p enhances tumorigenesis and metastasis of esophageal squamous cell carcinoma by targeting REPS2

Recently H19 has been demonstrated to be up-regulated in esophageal squamous cell carcinoma (ESCC) and shown to be the precursor of miR-675 that encodes miR-675-5p conservatively. miR-675 is overexpressed in many human cancers; however, the function of miR-675-5p is largely unknown in ESCC. In this...

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Autores principales: Zhou, Yan-Wu, Zhang, Hang, Duan, Chao-Jun, Gao, Yang, Cheng, Yuan-Da, He, Dan, Li, Rong, Zhang, Chun-Fang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5058713/
https://www.ncbi.nlm.nih.gov/pubmed/27120794
http://dx.doi.org/10.18632/oncotarget.8950
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author Zhou, Yan-Wu
Zhang, Hang
Duan, Chao-Jun
Gao, Yang
Cheng, Yuan-Da
He, Dan
Li, Rong
Zhang, Chun-Fang
author_facet Zhou, Yan-Wu
Zhang, Hang
Duan, Chao-Jun
Gao, Yang
Cheng, Yuan-Da
He, Dan
Li, Rong
Zhang, Chun-Fang
author_sort Zhou, Yan-Wu
collection PubMed
description Recently H19 has been demonstrated to be up-regulated in esophageal squamous cell carcinoma (ESCC) and shown to be the precursor of miR-675 that encodes miR-675-5p conservatively. miR-675 is overexpressed in many human cancers; however, the function of miR-675-5p is largely unknown in ESCC. In this study, we found that miR-675-5p expression was significantly increased in ESCC tissues and cell lines and related with ESCC progression and poor prognosis. We also showed here that down-regulation of miR-675-5p in ESCC cells dramatically induced cell G1 arrest and reduced cell proliferation, colony formation, migration and invasion in vitro as well as tumorigenesis and tumor metastasis in vivo. We subsequently identified that REPS2 was a target gene of miR-675-5p. We found that inhibition of miR-675-5p up-regulated the expression of REPS2, inhibited RalBP1/RAC1/CDC42 signaling pathway. Inversely, interference of REPS2 abrogated the effect induced by miR-675-5p inhibition, which resembled the function of miR-675-5p up-regulation. Taken together, our findings suggested that miR-675-5p might play an oncogenic role in ESCC through RalBP1/RAC1/CDC42 signaling pathway by inhibiting REPS2 and might serve as a valuable prognostic biomarker and therapeutic target for ESCC patients.
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spelling pubmed-50587132016-10-15 miR-675-5p enhances tumorigenesis and metastasis of esophageal squamous cell carcinoma by targeting REPS2 Zhou, Yan-Wu Zhang, Hang Duan, Chao-Jun Gao, Yang Cheng, Yuan-Da He, Dan Li, Rong Zhang, Chun-Fang Oncotarget Research Paper Recently H19 has been demonstrated to be up-regulated in esophageal squamous cell carcinoma (ESCC) and shown to be the precursor of miR-675 that encodes miR-675-5p conservatively. miR-675 is overexpressed in many human cancers; however, the function of miR-675-5p is largely unknown in ESCC. In this study, we found that miR-675-5p expression was significantly increased in ESCC tissues and cell lines and related with ESCC progression and poor prognosis. We also showed here that down-regulation of miR-675-5p in ESCC cells dramatically induced cell G1 arrest and reduced cell proliferation, colony formation, migration and invasion in vitro as well as tumorigenesis and tumor metastasis in vivo. We subsequently identified that REPS2 was a target gene of miR-675-5p. We found that inhibition of miR-675-5p up-regulated the expression of REPS2, inhibited RalBP1/RAC1/CDC42 signaling pathway. Inversely, interference of REPS2 abrogated the effect induced by miR-675-5p inhibition, which resembled the function of miR-675-5p up-regulation. Taken together, our findings suggested that miR-675-5p might play an oncogenic role in ESCC through RalBP1/RAC1/CDC42 signaling pathway by inhibiting REPS2 and might serve as a valuable prognostic biomarker and therapeutic target for ESCC patients. Impact Journals LLC 2016-04-23 /pmc/articles/PMC5058713/ /pubmed/27120794 http://dx.doi.org/10.18632/oncotarget.8950 Text en Copyright: © 2016 Zhou et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Zhou, Yan-Wu
Zhang, Hang
Duan, Chao-Jun
Gao, Yang
Cheng, Yuan-Da
He, Dan
Li, Rong
Zhang, Chun-Fang
miR-675-5p enhances tumorigenesis and metastasis of esophageal squamous cell carcinoma by targeting REPS2
title miR-675-5p enhances tumorigenesis and metastasis of esophageal squamous cell carcinoma by targeting REPS2
title_full miR-675-5p enhances tumorigenesis and metastasis of esophageal squamous cell carcinoma by targeting REPS2
title_fullStr miR-675-5p enhances tumorigenesis and metastasis of esophageal squamous cell carcinoma by targeting REPS2
title_full_unstemmed miR-675-5p enhances tumorigenesis and metastasis of esophageal squamous cell carcinoma by targeting REPS2
title_short miR-675-5p enhances tumorigenesis and metastasis of esophageal squamous cell carcinoma by targeting REPS2
title_sort mir-675-5p enhances tumorigenesis and metastasis of esophageal squamous cell carcinoma by targeting reps2
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5058713/
https://www.ncbi.nlm.nih.gov/pubmed/27120794
http://dx.doi.org/10.18632/oncotarget.8950
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