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A plasma membrane microdomain compartmentalizes ephrin-generated cAMP signals to prune developing retinal axon arbors
The development of neuronal circuits is controlled by guidance molecules that are hypothesized to interact with the cholesterol-enriched domains of the plasma membrane termed lipid rafts. Whether such domains enable local intracellular signalling at the submicrometre scale in developing neurons and...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5059439/ https://www.ncbi.nlm.nih.gov/pubmed/27694812 http://dx.doi.org/10.1038/ncomms12896 |
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author | Averaimo, Stefania Assali, Ahlem Ros, Oriol Couvet, Sandrine Zagar, Yvrick Genescu, Ioana Rebsam, Alexandra Nicol, Xavier |
author_facet | Averaimo, Stefania Assali, Ahlem Ros, Oriol Couvet, Sandrine Zagar, Yvrick Genescu, Ioana Rebsam, Alexandra Nicol, Xavier |
author_sort | Averaimo, Stefania |
collection | PubMed |
description | The development of neuronal circuits is controlled by guidance molecules that are hypothesized to interact with the cholesterol-enriched domains of the plasma membrane termed lipid rafts. Whether such domains enable local intracellular signalling at the submicrometre scale in developing neurons and are required for shaping the nervous system connectivity in vivo remains controversial. Here, we report a role for lipid rafts in generating domains of local cAMP signalling in axonal growth cones downstream of ephrin-A repulsive guidance cues. Ephrin-A-dependent retraction of retinal ganglion cell axons involves cAMP signalling restricted to the vicinity of lipid rafts and is independent of cAMP modulation outside of this microdomain. cAMP modulation near lipid rafts controls the pruning of ectopic axonal branches of retinal ganglion cells in vivo, a process requiring intact ephrin-A signalling. Together, our findings indicate that lipid rafts structure the subcellular organization of intracellular cAMP signalling shaping axonal arbors during the nervous system development. |
format | Online Article Text |
id | pubmed-5059439 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-50594392016-10-26 A plasma membrane microdomain compartmentalizes ephrin-generated cAMP signals to prune developing retinal axon arbors Averaimo, Stefania Assali, Ahlem Ros, Oriol Couvet, Sandrine Zagar, Yvrick Genescu, Ioana Rebsam, Alexandra Nicol, Xavier Nat Commun Article The development of neuronal circuits is controlled by guidance molecules that are hypothesized to interact with the cholesterol-enriched domains of the plasma membrane termed lipid rafts. Whether such domains enable local intracellular signalling at the submicrometre scale in developing neurons and are required for shaping the nervous system connectivity in vivo remains controversial. Here, we report a role for lipid rafts in generating domains of local cAMP signalling in axonal growth cones downstream of ephrin-A repulsive guidance cues. Ephrin-A-dependent retraction of retinal ganglion cell axons involves cAMP signalling restricted to the vicinity of lipid rafts and is independent of cAMP modulation outside of this microdomain. cAMP modulation near lipid rafts controls the pruning of ectopic axonal branches of retinal ganglion cells in vivo, a process requiring intact ephrin-A signalling. Together, our findings indicate that lipid rafts structure the subcellular organization of intracellular cAMP signalling shaping axonal arbors during the nervous system development. Nature Publishing Group 2016-10-03 /pmc/articles/PMC5059439/ /pubmed/27694812 http://dx.doi.org/10.1038/ncomms12896 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Averaimo, Stefania Assali, Ahlem Ros, Oriol Couvet, Sandrine Zagar, Yvrick Genescu, Ioana Rebsam, Alexandra Nicol, Xavier A plasma membrane microdomain compartmentalizes ephrin-generated cAMP signals to prune developing retinal axon arbors |
title | A plasma membrane microdomain compartmentalizes ephrin-generated cAMP signals to prune developing retinal axon arbors |
title_full | A plasma membrane microdomain compartmentalizes ephrin-generated cAMP signals to prune developing retinal axon arbors |
title_fullStr | A plasma membrane microdomain compartmentalizes ephrin-generated cAMP signals to prune developing retinal axon arbors |
title_full_unstemmed | A plasma membrane microdomain compartmentalizes ephrin-generated cAMP signals to prune developing retinal axon arbors |
title_short | A plasma membrane microdomain compartmentalizes ephrin-generated cAMP signals to prune developing retinal axon arbors |
title_sort | plasma membrane microdomain compartmentalizes ephrin-generated camp signals to prune developing retinal axon arbors |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5059439/ https://www.ncbi.nlm.nih.gov/pubmed/27694812 http://dx.doi.org/10.1038/ncomms12896 |
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