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Electroacupuncture Treatment Alleviates Central Poststroke Pain by Inhibiting Brain Neuronal Apoptosis and Aberrant Astrocyte Activation

Electroacupuncture (EA) is reported to effectively relieve the central poststroke pain (CPSP). However, the underlying mechanism remains unclear. The present study investigated the detailed mechanisms of action of EA treatment at different frequencies for CPSP. A CPSP model was established with a si...

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Autores principales: Tian, Gui-Hua, Tao, Shan-Shan, Chen, Man-Tang, Li, Yu-Sang, Li, You-Ping, Shang, Hong-Cai, Tang, Xiao-Yi, Chen, Jian-Xin, Tang, He-Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5059615/
https://www.ncbi.nlm.nih.gov/pubmed/27774321
http://dx.doi.org/10.1155/2016/1437148
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author Tian, Gui-Hua
Tao, Shan-Shan
Chen, Man-Tang
Li, Yu-Sang
Li, You-Ping
Shang, Hong-Cai
Tang, Xiao-Yi
Chen, Jian-Xin
Tang, He-Bin
author_facet Tian, Gui-Hua
Tao, Shan-Shan
Chen, Man-Tang
Li, Yu-Sang
Li, You-Ping
Shang, Hong-Cai
Tang, Xiao-Yi
Chen, Jian-Xin
Tang, He-Bin
author_sort Tian, Gui-Hua
collection PubMed
description Electroacupuncture (EA) is reported to effectively relieve the central poststroke pain (CPSP). However, the underlying mechanism remains unclear. The present study investigated the detailed mechanisms of action of EA treatment at different frequencies for CPSP. A CPSP model was established with a single collagenase injection to the left ventral posterolateral nucleus of the thalamus. The EA-treated groups then received EA treatment at frequency of 2, 2/15, or 15 Hz for 30 min daily for five days. The pain-related behavioral responses, neuronal apoptosis, glial activation, and the expression of pain signal transmission-related factors (β-catenin, COX-2, and NK-1R) were assessed using behavioral tests, Nissl staining, TUNEL staining, and immunohistochemical staining, respectively. The low-frequency EA treatment significantly (1) reduced brain tissue damage and hematoma sizes and (2) inhibited neuronal apoptosis, thereby exerting abirritative effects. Meanwhile, the high-frequency EA treatment induced a greater inhibition of the aberrant astrocyte activation, accompanied by the downregulation of the expressions of COX-2, β-catenin, and subsequently NK-1R, thereby alleviating inflammation and producing strong analgesic effects. Together, these findings suggest that CPSP is closely related to pathological changes of the neocortex and hippocampus. EA treatments at different frequencies may exert abirritative effects by inhibiting brain neuronal apoptosis and aberrant astrocyte activation in the brain.
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spelling pubmed-50596152016-10-23 Electroacupuncture Treatment Alleviates Central Poststroke Pain by Inhibiting Brain Neuronal Apoptosis and Aberrant Astrocyte Activation Tian, Gui-Hua Tao, Shan-Shan Chen, Man-Tang Li, Yu-Sang Li, You-Ping Shang, Hong-Cai Tang, Xiao-Yi Chen, Jian-Xin Tang, He-Bin Neural Plast Research Article Electroacupuncture (EA) is reported to effectively relieve the central poststroke pain (CPSP). However, the underlying mechanism remains unclear. The present study investigated the detailed mechanisms of action of EA treatment at different frequencies for CPSP. A CPSP model was established with a single collagenase injection to the left ventral posterolateral nucleus of the thalamus. The EA-treated groups then received EA treatment at frequency of 2, 2/15, or 15 Hz for 30 min daily for five days. The pain-related behavioral responses, neuronal apoptosis, glial activation, and the expression of pain signal transmission-related factors (β-catenin, COX-2, and NK-1R) were assessed using behavioral tests, Nissl staining, TUNEL staining, and immunohistochemical staining, respectively. The low-frequency EA treatment significantly (1) reduced brain tissue damage and hematoma sizes and (2) inhibited neuronal apoptosis, thereby exerting abirritative effects. Meanwhile, the high-frequency EA treatment induced a greater inhibition of the aberrant astrocyte activation, accompanied by the downregulation of the expressions of COX-2, β-catenin, and subsequently NK-1R, thereby alleviating inflammation and producing strong analgesic effects. Together, these findings suggest that CPSP is closely related to pathological changes of the neocortex and hippocampus. EA treatments at different frequencies may exert abirritative effects by inhibiting brain neuronal apoptosis and aberrant astrocyte activation in the brain. Hindawi Publishing Corporation 2016 2016-09-27 /pmc/articles/PMC5059615/ /pubmed/27774321 http://dx.doi.org/10.1155/2016/1437148 Text en Copyright © 2016 Gui-Hua Tian et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Tian, Gui-Hua
Tao, Shan-Shan
Chen, Man-Tang
Li, Yu-Sang
Li, You-Ping
Shang, Hong-Cai
Tang, Xiao-Yi
Chen, Jian-Xin
Tang, He-Bin
Electroacupuncture Treatment Alleviates Central Poststroke Pain by Inhibiting Brain Neuronal Apoptosis and Aberrant Astrocyte Activation
title Electroacupuncture Treatment Alleviates Central Poststroke Pain by Inhibiting Brain Neuronal Apoptosis and Aberrant Astrocyte Activation
title_full Electroacupuncture Treatment Alleviates Central Poststroke Pain by Inhibiting Brain Neuronal Apoptosis and Aberrant Astrocyte Activation
title_fullStr Electroacupuncture Treatment Alleviates Central Poststroke Pain by Inhibiting Brain Neuronal Apoptosis and Aberrant Astrocyte Activation
title_full_unstemmed Electroacupuncture Treatment Alleviates Central Poststroke Pain by Inhibiting Brain Neuronal Apoptosis and Aberrant Astrocyte Activation
title_short Electroacupuncture Treatment Alleviates Central Poststroke Pain by Inhibiting Brain Neuronal Apoptosis and Aberrant Astrocyte Activation
title_sort electroacupuncture treatment alleviates central poststroke pain by inhibiting brain neuronal apoptosis and aberrant astrocyte activation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5059615/
https://www.ncbi.nlm.nih.gov/pubmed/27774321
http://dx.doi.org/10.1155/2016/1437148
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