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Exercise Training Alleviates Hypoxia-induced Mitochondrial Dysfunction in the Lymphocytes of Sedentary Males
This study elucidates how interval and continuous exercise regimens affect the mitochondrial functionality of lymphocytes under hypoxic stress. Sixty healthy sedentary males were randomly assigned to engage in either high-intensity interval training (HIIT, 3 min intervals at 80% and 40% VO(2max), n ...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5059637/ https://www.ncbi.nlm.nih.gov/pubmed/27731374 http://dx.doi.org/10.1038/srep35170 |
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author | Tsai, Hsing-Hua Chang, Shao-Chiang Chou, Cheng-Hsien Weng, Tzu-Pin Hsu, Chih-Chin Wang, Jong-Shyan |
author_facet | Tsai, Hsing-Hua Chang, Shao-Chiang Chou, Cheng-Hsien Weng, Tzu-Pin Hsu, Chih-Chin Wang, Jong-Shyan |
author_sort | Tsai, Hsing-Hua |
collection | PubMed |
description | This study elucidates how interval and continuous exercise regimens affect the mitochondrial functionality of lymphocytes under hypoxic stress. Sixty healthy sedentary males were randomly assigned to engage in either high-intensity interval training (HIIT, 3 min intervals at 80% and 40% VO(2max), n = 20) or moderate-intensity continuous training (MICT, sustained 60% VO(2max), n = 20) for 30 min/day, 5 days/week for 6 weeks or were assigned to a control group that did not receive exercise intervention (n = 20). Lymphocyte phenotypes/mitochondrial functionality under hypoxic exercise (HE, 100 W under 12% O(2)) were determined before and after the various interventions. Before the intervention, HE (i) increased the mobilization of senescent (CD57(+)/CD28(−)) lymphocytes into the blood, (ii) decreased the ATP-linked O(2) consumption rate (OCR), the reserve capacity of OCR, and the citrate synthase activity in the mitochondria, and (iii) lowered the mitochondrial membrane potential (MP) and elevated the matrix oxidant burden (MOB) of lymphocytes. However, both HIIT and MICT significantly (i) decreased blood senescent lymphocyte counts, (ii) enhanced the mitochondrial OCR with increased citrate synthase and succinate dehydrogenase activities, (iii) increased mitochondrial MP and decreased MOB and (iv) increased the ratio of mitofusin to DRP-1 in lymphocytes after HE. Thus, we concluded that either HIIT or MICT effectively improves lymphocyte mitochondrial functionality by enhancing oxidative phosphorylation and suppressing oxidative damage under hypoxic conditions. |
format | Online Article Text |
id | pubmed-5059637 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-50596372016-10-24 Exercise Training Alleviates Hypoxia-induced Mitochondrial Dysfunction in the Lymphocytes of Sedentary Males Tsai, Hsing-Hua Chang, Shao-Chiang Chou, Cheng-Hsien Weng, Tzu-Pin Hsu, Chih-Chin Wang, Jong-Shyan Sci Rep Article This study elucidates how interval and continuous exercise regimens affect the mitochondrial functionality of lymphocytes under hypoxic stress. Sixty healthy sedentary males were randomly assigned to engage in either high-intensity interval training (HIIT, 3 min intervals at 80% and 40% VO(2max), n = 20) or moderate-intensity continuous training (MICT, sustained 60% VO(2max), n = 20) for 30 min/day, 5 days/week for 6 weeks or were assigned to a control group that did not receive exercise intervention (n = 20). Lymphocyte phenotypes/mitochondrial functionality under hypoxic exercise (HE, 100 W under 12% O(2)) were determined before and after the various interventions. Before the intervention, HE (i) increased the mobilization of senescent (CD57(+)/CD28(−)) lymphocytes into the blood, (ii) decreased the ATP-linked O(2) consumption rate (OCR), the reserve capacity of OCR, and the citrate synthase activity in the mitochondria, and (iii) lowered the mitochondrial membrane potential (MP) and elevated the matrix oxidant burden (MOB) of lymphocytes. However, both HIIT and MICT significantly (i) decreased blood senescent lymphocyte counts, (ii) enhanced the mitochondrial OCR with increased citrate synthase and succinate dehydrogenase activities, (iii) increased mitochondrial MP and decreased MOB and (iv) increased the ratio of mitofusin to DRP-1 in lymphocytes after HE. Thus, we concluded that either HIIT or MICT effectively improves lymphocyte mitochondrial functionality by enhancing oxidative phosphorylation and suppressing oxidative damage under hypoxic conditions. Nature Publishing Group 2016-10-12 /pmc/articles/PMC5059637/ /pubmed/27731374 http://dx.doi.org/10.1038/srep35170 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Tsai, Hsing-Hua Chang, Shao-Chiang Chou, Cheng-Hsien Weng, Tzu-Pin Hsu, Chih-Chin Wang, Jong-Shyan Exercise Training Alleviates Hypoxia-induced Mitochondrial Dysfunction in the Lymphocytes of Sedentary Males |
title | Exercise Training Alleviates Hypoxia-induced Mitochondrial Dysfunction in the Lymphocytes of Sedentary Males |
title_full | Exercise Training Alleviates Hypoxia-induced Mitochondrial Dysfunction in the Lymphocytes of Sedentary Males |
title_fullStr | Exercise Training Alleviates Hypoxia-induced Mitochondrial Dysfunction in the Lymphocytes of Sedentary Males |
title_full_unstemmed | Exercise Training Alleviates Hypoxia-induced Mitochondrial Dysfunction in the Lymphocytes of Sedentary Males |
title_short | Exercise Training Alleviates Hypoxia-induced Mitochondrial Dysfunction in the Lymphocytes of Sedentary Males |
title_sort | exercise training alleviates hypoxia-induced mitochondrial dysfunction in the lymphocytes of sedentary males |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5059637/ https://www.ncbi.nlm.nih.gov/pubmed/27731374 http://dx.doi.org/10.1038/srep35170 |
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