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Whole‐genome analysis of influenza A(H1N1)pdm09 viruses isolated in Uganda from 2009 to 2011
We report a whole‐genome analysis of 19 influenza A(H1N1)pdm09 isolates from four Ugandan hospitals between 2009 and 2011. The isolates differed from the vaccine strain A/California/07/2009 by three amino acid substitutions P100S, S220T, and I338V in the hemagglutinin and by two amino acid substitut...
| Autores principales: | , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
John Wiley and Sons Inc.
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5059949/ https://www.ncbi.nlm.nih.gov/pubmed/27339410 http://dx.doi.org/10.1111/irv.12401 |
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| author | Byarugaba, Denis K. Erima, Bernard Millard, Monica Kibuuka, Hannah Lkwago, Luswa Bwogi, Josephine Mimbe, Derrick Kiconco, Jocelyn B. Tugume, Titus Mworozi, Edison A. Turner, Jasmine Mckenzie, Pamela P. Webby, Richard R. J. Webster, Robert G. Foret, Charlotte Ducatez, Mariette F. Coldren, Rodney Wabwire‐Mangen, Fred Krauss, Scott |
| author_facet | Byarugaba, Denis K. Erima, Bernard Millard, Monica Kibuuka, Hannah Lkwago, Luswa Bwogi, Josephine Mimbe, Derrick Kiconco, Jocelyn B. Tugume, Titus Mworozi, Edison A. Turner, Jasmine Mckenzie, Pamela P. Webby, Richard R. J. Webster, Robert G. Foret, Charlotte Ducatez, Mariette F. Coldren, Rodney Wabwire‐Mangen, Fred Krauss, Scott |
| author_sort | Byarugaba, Denis K. |
| collection | PubMed |
| description | We report a whole‐genome analysis of 19 influenza A(H1N1)pdm09 isolates from four Ugandan hospitals between 2009 and 2011. The isolates differed from the vaccine strain A/California/07/2009 by three amino acid substitutions P100S, S220T, and I338V in the hemagglutinin and by two amino acid substitutions V106I and N248D in the neuraminidase proteins with consistent mutations in all gene segments distinguishing isolates from the 2009/2010 to 2010/2011 seasons. Phylogenetic analysis showed low genetic evolution, with genetic distances of 0%–1.3% and 0.1%–1.6% for HA and NA genes, respectively. The amino acid substitutions did not lead to antigenic differences from the reference strains. |
| format | Online Article Text |
| id | pubmed-5059949 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2016 |
| publisher | John Wiley and Sons Inc. |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-50599492016-11-01 Whole‐genome analysis of influenza A(H1N1)pdm09 viruses isolated in Uganda from 2009 to 2011 Byarugaba, Denis K. Erima, Bernard Millard, Monica Kibuuka, Hannah Lkwago, Luswa Bwogi, Josephine Mimbe, Derrick Kiconco, Jocelyn B. Tugume, Titus Mworozi, Edison A. Turner, Jasmine Mckenzie, Pamela P. Webby, Richard R. J. Webster, Robert G. Foret, Charlotte Ducatez, Mariette F. Coldren, Rodney Wabwire‐Mangen, Fred Krauss, Scott Influenza Other Respir Viruses Short Article We report a whole‐genome analysis of 19 influenza A(H1N1)pdm09 isolates from four Ugandan hospitals between 2009 and 2011. The isolates differed from the vaccine strain A/California/07/2009 by three amino acid substitutions P100S, S220T, and I338V in the hemagglutinin and by two amino acid substitutions V106I and N248D in the neuraminidase proteins with consistent mutations in all gene segments distinguishing isolates from the 2009/2010 to 2010/2011 seasons. Phylogenetic analysis showed low genetic evolution, with genetic distances of 0%–1.3% and 0.1%–1.6% for HA and NA genes, respectively. The amino acid substitutions did not lead to antigenic differences from the reference strains. John Wiley and Sons Inc. 2016-07-19 2016-11 /pmc/articles/PMC5059949/ /pubmed/27339410 http://dx.doi.org/10.1111/irv.12401 Text en © 2016 The Authors. Influenza and Other Respiratory Viruses Published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
| spellingShingle | Short Article Byarugaba, Denis K. Erima, Bernard Millard, Monica Kibuuka, Hannah Lkwago, Luswa Bwogi, Josephine Mimbe, Derrick Kiconco, Jocelyn B. Tugume, Titus Mworozi, Edison A. Turner, Jasmine Mckenzie, Pamela P. Webby, Richard R. J. Webster, Robert G. Foret, Charlotte Ducatez, Mariette F. Coldren, Rodney Wabwire‐Mangen, Fred Krauss, Scott Whole‐genome analysis of influenza A(H1N1)pdm09 viruses isolated in Uganda from 2009 to 2011 |
| title | Whole‐genome analysis of influenza A(H1N1)pdm09 viruses isolated in Uganda from 2009 to 2011 |
| title_full | Whole‐genome analysis of influenza A(H1N1)pdm09 viruses isolated in Uganda from 2009 to 2011 |
| title_fullStr | Whole‐genome analysis of influenza A(H1N1)pdm09 viruses isolated in Uganda from 2009 to 2011 |
| title_full_unstemmed | Whole‐genome analysis of influenza A(H1N1)pdm09 viruses isolated in Uganda from 2009 to 2011 |
| title_short | Whole‐genome analysis of influenza A(H1N1)pdm09 viruses isolated in Uganda from 2009 to 2011 |
| title_sort | whole‐genome analysis of influenza a(h1n1)pdm09 viruses isolated in uganda from 2009 to 2011 |
| topic | Short Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5059949/ https://www.ncbi.nlm.nih.gov/pubmed/27339410 http://dx.doi.org/10.1111/irv.12401 |
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