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Molecular cytogenetic analysis of early spontaneous abortions conceived from varying assisted reproductive technology procedures

BACKGROUND: Spontaneous abortion (SA) is the most common complication of pregnancy, and chromosome aberrations are the principal cause of the first trimester abortuses in natural conception (NC) The increasing use of assisted reproductive technology (ART) has raised concern about chromosome abnormal...

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Autores principales: Wu, Tonghua, Yin, Biao, Zhu, Yuanchang, Li, Guangui, Ye, Lijun, Chen, Chunmei, Zeng, Yong, Liang, Desheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5059961/
https://www.ncbi.nlm.nih.gov/pubmed/27752285
http://dx.doi.org/10.1186/s13039-016-0284-2
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author Wu, Tonghua
Yin, Biao
Zhu, Yuanchang
Li, Guangui
Ye, Lijun
Chen, Chunmei
Zeng, Yong
Liang, Desheng
author_facet Wu, Tonghua
Yin, Biao
Zhu, Yuanchang
Li, Guangui
Ye, Lijun
Chen, Chunmei
Zeng, Yong
Liang, Desheng
author_sort Wu, Tonghua
collection PubMed
description BACKGROUND: Spontaneous abortion (SA) is the most common complication of pregnancy, and chromosome aberrations are the principal cause of the first trimester abortuses in natural conception (NC) The increasing use of assisted reproductive technology (ART) has raised concern about chromosome abnormalities in ART-initiated pregnancies. Up to date, the literature on the risk of aneuploidy in failed pregnancies among various ART factors remain limited and inconclusive. This study aimed to explore the genetic etiology of pregnancy loss conceived from varying ART procedures. RESULTS: A total of 560 cases of villus that were successfully collected and performed molecular karyotype analysis were enrolled in present research, including 92 cases of NC, 340 cases of in-vitro fertilization (IVF) and 128 cases of intracytoplasmic sperm injection (ICSI). There was no statistical difference in the distribution of karyotyping results and the aneuploidy rate of each individual chromosome among NC, IVF and ICSI group. Both the total chromosomal abnormality rate and the one chromosome aneuploidy rate were increased with maternal age. Compared with fresh ET abortion group, frozen-thawed embryo transfer (FET) abortion group had elder maternal age (34.68 ± 4.73 years vs. 33.41 ± 4.48 years, P = 0.003) but lower chromosomal aberration rate of abortus (58.33 % vs. 67.50 %, P = 0.040). A slightly higher incidence of chromosome segmental abnormalities was found in FET than in fresh ET abortion (5.26 % vs. 2.08 %, P = 0.066). CONCLUSIONS: Chromosomal abnormality of fetus is the main cause of SA in the first trimester, no matter pregnancies are conceived through NC, IVF or ICSI. ART is a relatively safety treatment, and it does not enhance aneuploidy rate of abortus. The FET is bad for ongonging pregnancy and the aneuploidy rate were increased with maternal age.
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spelling pubmed-50599612016-10-17 Molecular cytogenetic analysis of early spontaneous abortions conceived from varying assisted reproductive technology procedures Wu, Tonghua Yin, Biao Zhu, Yuanchang Li, Guangui Ye, Lijun Chen, Chunmei Zeng, Yong Liang, Desheng Mol Cytogenet Research BACKGROUND: Spontaneous abortion (SA) is the most common complication of pregnancy, and chromosome aberrations are the principal cause of the first trimester abortuses in natural conception (NC) The increasing use of assisted reproductive technology (ART) has raised concern about chromosome abnormalities in ART-initiated pregnancies. Up to date, the literature on the risk of aneuploidy in failed pregnancies among various ART factors remain limited and inconclusive. This study aimed to explore the genetic etiology of pregnancy loss conceived from varying ART procedures. RESULTS: A total of 560 cases of villus that were successfully collected and performed molecular karyotype analysis were enrolled in present research, including 92 cases of NC, 340 cases of in-vitro fertilization (IVF) and 128 cases of intracytoplasmic sperm injection (ICSI). There was no statistical difference in the distribution of karyotyping results and the aneuploidy rate of each individual chromosome among NC, IVF and ICSI group. Both the total chromosomal abnormality rate and the one chromosome aneuploidy rate were increased with maternal age. Compared with fresh ET abortion group, frozen-thawed embryo transfer (FET) abortion group had elder maternal age (34.68 ± 4.73 years vs. 33.41 ± 4.48 years, P = 0.003) but lower chromosomal aberration rate of abortus (58.33 % vs. 67.50 %, P = 0.040). A slightly higher incidence of chromosome segmental abnormalities was found in FET than in fresh ET abortion (5.26 % vs. 2.08 %, P = 0.066). CONCLUSIONS: Chromosomal abnormality of fetus is the main cause of SA in the first trimester, no matter pregnancies are conceived through NC, IVF or ICSI. ART is a relatively safety treatment, and it does not enhance aneuploidy rate of abortus. The FET is bad for ongonging pregnancy and the aneuploidy rate were increased with maternal age. BioMed Central 2016-10-12 /pmc/articles/PMC5059961/ /pubmed/27752285 http://dx.doi.org/10.1186/s13039-016-0284-2 Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Wu, Tonghua
Yin, Biao
Zhu, Yuanchang
Li, Guangui
Ye, Lijun
Chen, Chunmei
Zeng, Yong
Liang, Desheng
Molecular cytogenetic analysis of early spontaneous abortions conceived from varying assisted reproductive technology procedures
title Molecular cytogenetic analysis of early spontaneous abortions conceived from varying assisted reproductive technology procedures
title_full Molecular cytogenetic analysis of early spontaneous abortions conceived from varying assisted reproductive technology procedures
title_fullStr Molecular cytogenetic analysis of early spontaneous abortions conceived from varying assisted reproductive technology procedures
title_full_unstemmed Molecular cytogenetic analysis of early spontaneous abortions conceived from varying assisted reproductive technology procedures
title_short Molecular cytogenetic analysis of early spontaneous abortions conceived from varying assisted reproductive technology procedures
title_sort molecular cytogenetic analysis of early spontaneous abortions conceived from varying assisted reproductive technology procedures
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5059961/
https://www.ncbi.nlm.nih.gov/pubmed/27752285
http://dx.doi.org/10.1186/s13039-016-0284-2
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