Bovine annulus fibrosus cell lines isolated from intervertebral discs

The adult bovine (Bos taurus) intervertebral disc is primarily comprised of two major tissue types: The outer annulus fibrosus (AF) and the central nucleus pulposus (NP). We isolated several primary cell lineages of passage (P) 0 cells from the AF tissue omitting typically used enzymatic tissue dige...

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Detalles Bibliográficos
Autores principales: Kraus, Petra, Lufkin, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Lab Resource
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5061063/
https://www.ncbi.nlm.nih.gov/pubmed/27752468
http://dx.doi.org/10.1016/j.gdata.2016.09.012
Descripción
Sumario:The adult bovine (Bos taurus) intervertebral disc is primarily comprised of two major tissue types: The outer annulus fibrosus (AF) and the central nucleus pulposus (NP). We isolated several primary cell lineages of passage (P) 0 cells from the AF tissue omitting typically used enzymatic tissue digestion protocols. The cells grow past p10 without signs of senescence in DMEM + 10% FCS on 0.1% gelatin coated/uncoated surfaces of standard cell culture plates and survive freeze-thawing. Preliminary analysis of the AF derived cells for expression of the two structural genes Col1a1 and Col2a1 was performed by PISH recapitulating the expression observed in vivo.

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