Bovine annulus fibrosus cell lines isolated from intervertebral discs

The adult bovine (Bos taurus) intervertebral disc is primarily comprised of two major tissue types: The outer annulus fibrosus (AF) and the central nucleus pulposus (NP). We isolated several primary cell lineages of passage (P) 0 cells from the AF tissue omitting typically used enzymatic tissue dige...

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Detalles Bibliográficos
Autores principales: Kraus, Petra, Lufkin, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Lab Resource
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5061063/
https://www.ncbi.nlm.nih.gov/pubmed/27752468
http://dx.doi.org/10.1016/j.gdata.2016.09.012
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author Kraus, Petra
Lufkin, Thomas
author_facet Kraus, Petra
Lufkin, Thomas
author_sort Kraus, Petra
collection PubMed
description The adult bovine (Bos taurus) intervertebral disc is primarily comprised of two major tissue types: The outer annulus fibrosus (AF) and the central nucleus pulposus (NP). We isolated several primary cell lineages of passage (P) 0 cells from the AF tissue omitting typically used enzymatic tissue digestion protocols. The cells grow past p10 without signs of senescence in DMEM + 10% FCS on 0.1% gelatin coated/uncoated surfaces of standard cell culture plates and survive freeze-thawing. Preliminary analysis of the AF derived cells for expression of the two structural genes Col1a1 and Col2a1 was performed by PISH recapitulating the expression observed in vivo.
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spelling pubmed-50610632016-10-17 Bovine annulus fibrosus cell lines isolated from intervertebral discs Kraus, Petra Lufkin, Thomas Genom Data Lab Resource The adult bovine (Bos taurus) intervertebral disc is primarily comprised of two major tissue types: The outer annulus fibrosus (AF) and the central nucleus pulposus (NP). We isolated several primary cell lineages of passage (P) 0 cells from the AF tissue omitting typically used enzymatic tissue digestion protocols. The cells grow past p10 without signs of senescence in DMEM + 10% FCS on 0.1% gelatin coated/uncoated surfaces of standard cell culture plates and survive freeze-thawing. Preliminary analysis of the AF derived cells for expression of the two structural genes Col1a1 and Col2a1 was performed by PISH recapitulating the expression observed in vivo. Elsevier 2016-10-03 /pmc/articles/PMC5061063/ /pubmed/27752468 http://dx.doi.org/10.1016/j.gdata.2016.09.012 Text en © 2016 Stellenbosch University http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Lab Resource
Kraus, Petra
Lufkin, Thomas
Bovine annulus fibrosus cell lines isolated from intervertebral discs
title Bovine annulus fibrosus cell lines isolated from intervertebral discs
title_full Bovine annulus fibrosus cell lines isolated from intervertebral discs
title_fullStr Bovine annulus fibrosus cell lines isolated from intervertebral discs
title_full_unstemmed Bovine annulus fibrosus cell lines isolated from intervertebral discs
title_short Bovine annulus fibrosus cell lines isolated from intervertebral discs
title_sort bovine annulus fibrosus cell lines isolated from intervertebral discs
topic Lab Resource
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5061063/
https://www.ncbi.nlm.nih.gov/pubmed/27752468
http://dx.doi.org/10.1016/j.gdata.2016.09.012
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