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Effects of herbal medicine on human uterine tumor-bearing nude mice

AIM: Uterine leiomyomas are the most common benign uterine neoplasms associated with significant morbidity. Herbal formulas capable of restoring yin-yang balance by dispersing blood stasis may be useful for managing fibroid symptoms. MATERIALS AND METHODS: In this study, the antitumor properties of...

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Autores principales: Ohh, Mi Hyang, Kim, Seong Jin, Han, Jong Kwon, Pak, Sok Cheon, Chee, Kew-mahn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGEYA 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5061487/
https://www.ncbi.nlm.nih.gov/pubmed/27757274
http://dx.doi.org/10.5455/jice.20160923100120
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author Ohh, Mi Hyang
Kim, Seong Jin
Han, Jong Kwon
Pak, Sok Cheon
Chee, Kew-mahn
author_facet Ohh, Mi Hyang
Kim, Seong Jin
Han, Jong Kwon
Pak, Sok Cheon
Chee, Kew-mahn
author_sort Ohh, Mi Hyang
collection PubMed
description AIM: Uterine leiomyomas are the most common benign uterine neoplasms associated with significant morbidity. Herbal formulas capable of restoring yin-yang balance by dispersing blood stasis may be useful for managing fibroid symptoms. MATERIALS AND METHODS: In this study, the antitumor properties of three herbs viz., Trogopterus xanthipes Milen-Edwards, Paeonia lactiflora Pallas, and Ulmus davidiana Planch were evaluated in nude mice injected intravenously with human malignant myomas. Tumor fragments were xenografted subcutaneously through a flank incision in female mice. The mice entered the study for 8 weeks when their tumors reached the threshold volume (260 mm(3)). The mice were randomly allocated to receive subcutaneous injections of normal saline (Group 1; negative control), P. lactiflora Pallas (Group 2), U. davidiana Planch (Group 3), T. xanthipes Milen-Edwards (Group 4), and intravenous injections of paclitaxel (Group 5; positive control). The weight and tumor volume were measured, followed by histopathology. RESULTS: A few cases of abdominal distention and death were observed in the negative control group. Furthermore, a considerable enlargement of the liver and spleen was observed in the negative control group at autopsy with a gradual increase in body weight during the experiment. The mean tumor volume which increased in negative control mice reduced in mice treated with herbal remedies or paclitaxel from day 14 onwards (P < 0.05). The degree of necrosis and apoptosis induction from herbal treatments was similar to that of paclitaxel. CONCLUSION: Collectively, three herbs viz., T. xanthipes Milen-Edwards, P. lactiflora Pallas, and U. davidiana Planch were able to induce necrosis and apoptosis of uterine leiomyoma cells, proving antitumor properties against uterine fibroids.
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spelling pubmed-50614872016-10-18 Effects of herbal medicine on human uterine tumor-bearing nude mice Ohh, Mi Hyang Kim, Seong Jin Han, Jong Kwon Pak, Sok Cheon Chee, Kew-mahn J Intercult Ethnopharmacol Original Research AIM: Uterine leiomyomas are the most common benign uterine neoplasms associated with significant morbidity. Herbal formulas capable of restoring yin-yang balance by dispersing blood stasis may be useful for managing fibroid symptoms. MATERIALS AND METHODS: In this study, the antitumor properties of three herbs viz., Trogopterus xanthipes Milen-Edwards, Paeonia lactiflora Pallas, and Ulmus davidiana Planch were evaluated in nude mice injected intravenously with human malignant myomas. Tumor fragments were xenografted subcutaneously through a flank incision in female mice. The mice entered the study for 8 weeks when their tumors reached the threshold volume (260 mm(3)). The mice were randomly allocated to receive subcutaneous injections of normal saline (Group 1; negative control), P. lactiflora Pallas (Group 2), U. davidiana Planch (Group 3), T. xanthipes Milen-Edwards (Group 4), and intravenous injections of paclitaxel (Group 5; positive control). The weight and tumor volume were measured, followed by histopathology. RESULTS: A few cases of abdominal distention and death were observed in the negative control group. Furthermore, a considerable enlargement of the liver and spleen was observed in the negative control group at autopsy with a gradual increase in body weight during the experiment. The mean tumor volume which increased in negative control mice reduced in mice treated with herbal remedies or paclitaxel from day 14 onwards (P < 0.05). The degree of necrosis and apoptosis induction from herbal treatments was similar to that of paclitaxel. CONCLUSION: Collectively, three herbs viz., T. xanthipes Milen-Edwards, P. lactiflora Pallas, and U. davidiana Planch were able to induce necrosis and apoptosis of uterine leiomyoma cells, proving antitumor properties against uterine fibroids. SAGEYA 2016-09-25 /pmc/articles/PMC5061487/ /pubmed/27757274 http://dx.doi.org/10.5455/jice.20160923100120 Text en Copyright: © SAGEYA http://creativecommons.org/licenses/by-nc/3.0/ This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, noncommercial use, distribution and reproduction in any medium, provided the work is properly cited.
spellingShingle Original Research
Ohh, Mi Hyang
Kim, Seong Jin
Han, Jong Kwon
Pak, Sok Cheon
Chee, Kew-mahn
Effects of herbal medicine on human uterine tumor-bearing nude mice
title Effects of herbal medicine on human uterine tumor-bearing nude mice
title_full Effects of herbal medicine on human uterine tumor-bearing nude mice
title_fullStr Effects of herbal medicine on human uterine tumor-bearing nude mice
title_full_unstemmed Effects of herbal medicine on human uterine tumor-bearing nude mice
title_short Effects of herbal medicine on human uterine tumor-bearing nude mice
title_sort effects of herbal medicine on human uterine tumor-bearing nude mice
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5061487/
https://www.ncbi.nlm.nih.gov/pubmed/27757274
http://dx.doi.org/10.5455/jice.20160923100120
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