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SpyB, a Small Heme-Binding Protein, Affects the Composition of the Cell Wall in Streptococcus pyogenes
Streptococcus pyogenes (Group A Streptococcus or GAS) is a hemolytic human pathogen associated with a wide variety of infections ranging from minor skin and throat infections to life-threatening invasive diseases. The cell wall of GAS consists of peptidoglycan sacculus decorated with a carbohydrate...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5061733/ https://www.ncbi.nlm.nih.gov/pubmed/27790410 http://dx.doi.org/10.3389/fcimb.2016.00126 |
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author | Edgar, Rebecca J. Chen, Jing Kant, Sashi Rechkina, Elena Rush, Jeffrey S. Forsberg, Lennart S. Jaehrig, Bernhard Azadi, Parastoo Tchesnokova, Veronika Sokurenko, Evgeni V. Zhu, Haining Korotkov, Konstantin V. Pancholi, Vijay Korotkova, Natalia |
author_facet | Edgar, Rebecca J. Chen, Jing Kant, Sashi Rechkina, Elena Rush, Jeffrey S. Forsberg, Lennart S. Jaehrig, Bernhard Azadi, Parastoo Tchesnokova, Veronika Sokurenko, Evgeni V. Zhu, Haining Korotkov, Konstantin V. Pancholi, Vijay Korotkova, Natalia |
author_sort | Edgar, Rebecca J. |
collection | PubMed |
description | Streptococcus pyogenes (Group A Streptococcus or GAS) is a hemolytic human pathogen associated with a wide variety of infections ranging from minor skin and throat infections to life-threatening invasive diseases. The cell wall of GAS consists of peptidoglycan sacculus decorated with a carbohydrate comprising a polyrhamnose backbone with immunodominant N-acetylglucosamine side-chains. All GAS genomes contain the spyBA operon, which encodes a 35-amino-acid membrane protein SpyB, and a membrane-bound C3-like ADP-ribosyltransferase SpyA. In this study, we addressed the function of SpyB in GAS. Phenotypic analysis of a spyB deletion mutant revealed increased bacterial aggregation, and reduced sensitivity to β-lactams of the cephalosporin class and peptidoglycan hydrolase PlyC. Glycosyl composition analysis of cell wall isolated from the spyB mutant suggested an altered carbohydrate structure compared with the wild-type strain. Furthermore, we found that SpyB associates with heme and protoporphyrin IX. Heme binding induces SpyB dimerization, which involves disulfide bond formation between the subunits. Thus, our data suggest the possibility that SpyB activity is regulated by heme. |
format | Online Article Text |
id | pubmed-5061733 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-50617332016-10-27 SpyB, a Small Heme-Binding Protein, Affects the Composition of the Cell Wall in Streptococcus pyogenes Edgar, Rebecca J. Chen, Jing Kant, Sashi Rechkina, Elena Rush, Jeffrey S. Forsberg, Lennart S. Jaehrig, Bernhard Azadi, Parastoo Tchesnokova, Veronika Sokurenko, Evgeni V. Zhu, Haining Korotkov, Konstantin V. Pancholi, Vijay Korotkova, Natalia Front Cell Infect Microbiol Microbiology Streptococcus pyogenes (Group A Streptococcus or GAS) is a hemolytic human pathogen associated with a wide variety of infections ranging from minor skin and throat infections to life-threatening invasive diseases. The cell wall of GAS consists of peptidoglycan sacculus decorated with a carbohydrate comprising a polyrhamnose backbone with immunodominant N-acetylglucosamine side-chains. All GAS genomes contain the spyBA operon, which encodes a 35-amino-acid membrane protein SpyB, and a membrane-bound C3-like ADP-ribosyltransferase SpyA. In this study, we addressed the function of SpyB in GAS. Phenotypic analysis of a spyB deletion mutant revealed increased bacterial aggregation, and reduced sensitivity to β-lactams of the cephalosporin class and peptidoglycan hydrolase PlyC. Glycosyl composition analysis of cell wall isolated from the spyB mutant suggested an altered carbohydrate structure compared with the wild-type strain. Furthermore, we found that SpyB associates with heme and protoporphyrin IX. Heme binding induces SpyB dimerization, which involves disulfide bond formation between the subunits. Thus, our data suggest the possibility that SpyB activity is regulated by heme. Frontiers Media S.A. 2016-10-13 /pmc/articles/PMC5061733/ /pubmed/27790410 http://dx.doi.org/10.3389/fcimb.2016.00126 Text en Copyright © 2016 Edgar, Chen, Kant, Rechkina, Rush, Forsberg, Jaehrig, Azadi, Tchesnokova, Sokurenko, Zhu, Korotkov, Pancholi and Korotkova. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Edgar, Rebecca J. Chen, Jing Kant, Sashi Rechkina, Elena Rush, Jeffrey S. Forsberg, Lennart S. Jaehrig, Bernhard Azadi, Parastoo Tchesnokova, Veronika Sokurenko, Evgeni V. Zhu, Haining Korotkov, Konstantin V. Pancholi, Vijay Korotkova, Natalia SpyB, a Small Heme-Binding Protein, Affects the Composition of the Cell Wall in Streptococcus pyogenes |
title | SpyB, a Small Heme-Binding Protein, Affects the Composition of the Cell Wall in Streptococcus pyogenes |
title_full | SpyB, a Small Heme-Binding Protein, Affects the Composition of the Cell Wall in Streptococcus pyogenes |
title_fullStr | SpyB, a Small Heme-Binding Protein, Affects the Composition of the Cell Wall in Streptococcus pyogenes |
title_full_unstemmed | SpyB, a Small Heme-Binding Protein, Affects the Composition of the Cell Wall in Streptococcus pyogenes |
title_short | SpyB, a Small Heme-Binding Protein, Affects the Composition of the Cell Wall in Streptococcus pyogenes |
title_sort | spyb, a small heme-binding protein, affects the composition of the cell wall in streptococcus pyogenes |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5061733/ https://www.ncbi.nlm.nih.gov/pubmed/27790410 http://dx.doi.org/10.3389/fcimb.2016.00126 |
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